Multipotent cells from the human third molar: feasibility of cell-based therapy for liver disease

Ikeda, Etsuko; Yagi, Kiyohito; Kojima, Masashi; Yagyuu, Takahiro; Ohshima, Akira; Sobajima, Satoshi; Tadokoro, Mika; Katsube, Yoshihiro; Isoda, Katsuhiro; Kondoh, Masuo; Kawase, Masaya; Go, Masahiro J.; Adachi, Hisashi; Yokota, Yukiharu; Kirita, Tadaaki; Ohgushi, Hajime

doi:10.1111/j.1432-0436.2007.00245.x

Cited by 184 publications

(170 citation statements)

References 26 publications

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“…Isolation of multipotent MSC-like cells from dental follicles, dental pulp, dental papilla and peri-odontal ligaments, all of which originate from the tooth germ, has been successfully achieved by various groups, including our laboratory. [17][18][19][20]23,24,[26][27][28] Unlike earlier reports, in this study we isolated stem cells for the first time from the entire tooth germ, which includes aforementioned tissues. Although we did not compare side-by-side, this approach yielded higher numbers of cells, showing a higher proliferation rate compared with the dental follicle cells we isolated earlier.…”

Section: Discussionmentioning

confidence: 99%

“…13 Figure 6 Real-time PCR analysis of oct4 messenger RNA (mRNA) expression levels in human tooth germ stem cells (hTGSCs) in response to increased number of passages (1,3,27) and osteogenic and neurogenic differentiation. The level of oct4 messenger RNA (mRNA) was over 10-times less in hTGSCs when compared with embryonic stem cells, and its expression further decreased significantly after the first passage.…”

Section: Figurementioning

confidence: 99%

“…13 Multipotent tooth germ progenitor cells derived from the dental papilla of developing third molars have been successfully differentiated into ecto-dermal and meso-dermal cells and have shown a therapeutic effect on damaged liver. 27 In a recent study, Takeda et al 28 reported that human dental pulp stem cells isolated from earlier stage (crown-completed) tooth development showed higher proliferation rates than those isolated at a later stage (rootcompleted). However, both cell types showed similar osteo/ odonto-blastic differentiation efficiency.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

et al. 2009

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A number of studies have reported in the last decade that human tooth germs contain multipotent cells that give rise to dental and peri-odontal structures. The dental pulp, third molars in particular, have been shown to be a significant stem cell source. In this study, we isolated and characterized human tooth germ stem cells (hTGSCs) from third molars and assessed the expression of developmentally important transcription factors, such as oct4, sox2, klf4, nanog and c-myc, to determine their pluri-potency. Flowcytometry analysis revealed that hTGSCs were positive for CD73, CD90, CD105 and CD166, but negative for CD34, CD45 and CD133, suggesting that these cells are mesenchymal-like stem cells. Under specific culture conditions, hTGSCs differentiated into osteogenic, adipogenic and neurogenic cells, as well as formed tube-like structures in Matrigel assay. hTGSCs showed significant levels of expression of sox2 and c-myc messenger RNA (mRNA), and a very high level of expression of klf4 mRNA when compared with human embryonic stem cells. This study reports for the first time that hTGSCs express developmentally important transcription factors that could render hTGSCs an attractive candidate for future somatic cell re-programming studies to differentiate germs into various tissue types, such as neurons and vascular structures. In addition, these multipotential hTGSCs could be important stem cell sources for autologous transplantation.

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Section: Discussionmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

et al. 2009

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“…In a previous paper, we reported the identification and isolation of an odontogenic progenitor population from the dental pulp tissue of adult rats using a culture system with a medium containing bFGF (Morito et al, 2005). Multipotent tooth germ progenitor cells might also be one of the candidates for regenerative cell therapy to treat liver diseases (Ikeda et al, 2008). Consequently, the dental pulp might hold promise as a better source of adult stem cells than bone marrow.…”

Section: Introductionmentioning

confidence: 99%

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Morito

Kida

Suzuki

et al. 2009

Archives of Histology and Cytology

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in the presence of bFGF into immunocompromised mice revealed the formation of bone, cartilage, and adipose tissue. The donor hDPC-derived cells were labeled in the bone tissues located near the PLGA in the subcutaneous tissues of recipient mice using a humanspecific Alu probe. When cultured with a serum-free medium containing bFGF, the hDPCs strongly expressed STRO-1 immunoreactive products and sustained selfrenewal, and thus were almost identical in differentiation potential and proliferation activity to hDPCs cultured with the medium containing serum and bFGF. The present results suggest that the hDPCs cultured in the presence of bFGF irrespective of the presence or absence of the bovine serum are rich in mesenchymal stem cells or progenitor cells and useful for cell-based therapies to treat dental diseases.

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“…Hence, mesenchymal stem cells (MSCs) obtained from almost all postnatal organs such as bone marrow, adipose tissue, synovial membrane, skin and dental pulp are of great interest (da Silva Meirelles et al 2006). Among them, human tooth germ stem cells (hTGSCs) isolated from tooth germs of young adults have been shown to exert remarkable mesenchymal characteristics and differentiate into cell lineages originated from three germ layers (Ikeda et al 2008). Multipotency and highly proliferative properties of these stem cells may be explained by quiescent and undifferentiated state of tooth germs until the age of six (Kerkis et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Myogenic and neurogenic differentiation of human tooth germ stem cells (hTGSCs) are regulated by pluronic block copolymers

et al. 2015

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Stem cells with high proliferation, selfrenewal and differentiation capacities are promising for tissue engineering approaches. Among stem cells, human tooth germ stem cells (hTGSCs) having mesenchymal stem cell characteristics are highly proliferative and able to differentiate into several cell lineages. Researchers have recently focused on transplanting stem cells with bioconductive and/or bioinductive materials that can provide cell commitment to the desired cell lineages. In the present study, effects of pluronic block copolymers (F68, F127 and P85) on in vitro myo-and neurogenic differentiation of human tooth germ stem cells (hTGSCs) were investigated. As P85 was found to exert considerable toxicity to hTGSCs even at low concentrations, it was not evaluated for further differentiation experiments. Immunocytochemical analysis, gene and protein expression studies revealed that while F68 treatment increased lineage-specific gene expression in both myo-and neuro-genically differentiated cells, F127 did not result in any remarkable difference compared to cells treated with differentiation medium. Subsequent studies are required to explore the exact mechanisms of how F68 increases the myogenic and neurogenic differentiation of hTGSCs. The present work indicates that pluronic F68 might be used in functional skeletal and neural tissue engineering applications.

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Multipotent cells from the human third molar: feasibility of cell-based therapy for liver disease

Cited by 184 publications

References 26 publications

Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

Effects of basic fibroblast growth factor on the development of the stem cell properties of human dental pulp cells

Myogenic and neurogenic differentiation of human tooth germ stem cells (hTGSCs) are regulated by pluronic block copolymers

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