Multipotent Cell Fate of Neural Crest-Like Cells Derived from Embryonic Stem Cells

Motohashi, Tsutomu; Aoki, Hitomi; Chiba, Kairi; Yoshimura, Naoko; Kunisada, Takahiro

doi:10.1634/stemcells.2006-0323

Cited by 70 publications

(59 citation statements)

References 40 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One hundred Sox10þ/KitÀ and Sox10þ/Kitþ cells were isolated from day-9, -10, and -12 cultures and re-cultured on ST2 monolayers. After 21 days, colonies containing pigmented M were generated from Sox10þ/Kitþ cells isolated at days 9, 10, and 12 of the culture period, as previously reported (Motohashi et al, 2007). Surprisingly, Sox10þ/KitÀ cells also formed colonies containing pigmented M. Sox10-/ Kit-cells and Sox10-/Kitþ cells from day-9, -10, and -12 cultures did not form colonies containing M (data not shown).…”

Section: Generation Of Sox10-iresvenus Es Cellssupporting

confidence: 82%

“…The cells were isolated as Kit-expressing cells and had characteristics of NC cells in vivo, i.e., high motility and multipotency (Motohashi et al, 2007). Furthermore, we have also previously shown that Kitpositive melanoblasts, even after they have already migrated throughout the skin, are multipotent and able to generate N, G, and smooth muscle cells in addition to M (Motohashi et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…When ES cells are co-cultured with ST2 stromal cells, NC-like cells can be discriminated as Kitexpressing cells in the ES cell cultures. These NC-like cells differentiate into autonomic N, G, smooth muscle cells, and M, just like NC cells in the trunk do in vivo (Motohashi et al, 2007). In this present study, we further analyzed the early developmental commitment of the NC-like cells by using mouse ES cells designed to express green fluorescent protein under the control of the Sox10 promoter.…”

Section: Introductionmentioning

confidence: 91%

“…Previously, we reported the generation of NC-like cells from mouse ES cells (Motohashi et al, 2007). NC-like cells were isolated as Kit-expressing cells by flow cytometry, and they differentiated into N, G, and M, suggesting that these Kit-expressing cells have multipotency.…”

Section: Generation Of Sox10-iresvenus Es Cellsmentioning

confidence: 97%

“…We previously reported that NC-like cells could be generated from mouse ES cells in in vitro culture (Motohashi et al, 2007). When ES cells are co-cultured with ST2 stromal cells, NC-like cells can be discriminated as Kitexpressing cells in the ES cell cultures.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

Motohashi

Yamanaka

Chiba

et al. 2011

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

Multipotency of neural crest cells (NC cells) is thought to be a transient phase at the early stage of their generation; after NC cells emerge from the neural tube, they are specified into the lineage‐restricted precursors. We analyzed the differentiation of early‐stage NC‐like cells derived from Sox10‐IRES‐Venus ES cells, where the expression of Sox10 can be visualized with a fluorescent protein. Unexpectedly, both the Sox10+/Kit− cells and the Sox10+/Kit+ cells, which were restricted in vivo to the neuron (N)‐glial cell (G) lineage and melanocyte (M) lineage, respectively, generated N, G, and M, showing that they retain multipotency. We generated mice from the Sox10‐IRES‐Venus ES cells and analyzed the differentiation of their NC cells. Both the Sox10+/Kit− cells and Sox10+/Kit+ cells isolated from these mice formed colonies containing N, G, and M, showing that they are also multipotent. These findings suggest that NC cells retain multipotency even after the initial lineage‐restricted stages. Developmental Dynamics 240:1681–1693, 2011. © 2011 Wiley‐Liss, Inc.

show abstract

Section: Generation Of Sox10-iresvenus Es Cellssupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Generation Of Sox10-iresvenus Es Cellsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

Motohashi

Yamanaka

Chiba

et al. 2011

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

show abstract

The stemness of neural crest cells and their derivatives

Kunisada

Tezulka

Aoki

et al. 2014

Birth Defects Research Pt C

Self Cite

View full text Add to dashboard Cite

Neural crest cells (NCCs) are unique to vertebrates and emerge from the border of the neural plate and subsequently migrate extensively throughout the embryo after which they differentiate into many types of cells. This multipotency is the main reason why NCCs are regarded as a versatile tool for stem cell biology and have been gathering attention for their potential use in stem cell based therapy. Multiple sets of networks comprised of signaling molecules and transcription factors regulate every developmental phase of NCCs, including maintenance of their multipotency. Pluripotent stem cell lines, such as embryonic stem cells and induced pluripotent stem (iPS) cells, facilitate the induction of NCCs in combination with sophisticated culture systems used for neural stem cells, although at present, clinical experiments for NCC-based cell therapy need to be improved. Unexpectedly, the multipotency of NCCs is maintained after they reach the target tissues as tissue neural crest stem cells (NCSCs) that may contribute to the establishment of NCC-derived multipotential stem cells. In addition, under specific culture conditions, fate-restricted unipotent descendants of NCCs, such as melanoblasts, show multipotency to differentiate into melanocytes, neurons, and glia cells. These properties contribute to the additional versatility of NCCs for therapeutic application and to better understand NCC development.

show abstract

The chick embryo: hatching a model for contemporary biomedical research

Rashidi

Sottile

2009

BioEssays

View full text Add to dashboard Cite

Animal models play a crucial role in fundamental and medical research. Progress in the fields of drug discovery, regenerative medicine and cancer research among others are heavily dependent on in vivo models to validate in vitro observations, and develop new therapeutic approaches. However, conventional rodent and large animal experiments face ethical, practical and technical issues that limit their usage. The chick embryo represents an accessible and economical in vivo model, which has long been used in developmental biology, gene expression analysis and loss/gain of function experiments. It is also an established model for tissue/cell transplantation, and because of its lack of immune system in early development, the chick embryo is increasingly recognised as a model of choice for mammalian biology with new applications for stem cell and cancer research. Here, we review novel applications of the chick embryo model, and discuss future developments of this in vivo model for biomedical research.

show abstract

Multipotent Cell Fate of Neural Crest-Like Cells Derived from Embryonic Stem Cells

Abstract: STEM CELLS 2007;25:402-410

Cited by 70 publications

References 40 publications

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

Neural crest cells retain their capability for multipotential differentiation even after lineage‐restricted stages

The stemness of neural crest cells and their derivatives

The chick embryo: hatching a model for contemporary biomedical research

Contact Info

Product

Resources

About