MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled‐coil protein of <i>Arabidopsis thaliana</i>, is required for meiotic spindle organization

Jiang, Hua; Wang, Feifei; Wu, Yuting; Zhou, Xi; Huang, Xueyong; Zhu, Jun; Gao, Jufang; Rui-bin, Dong; Chen, Kun; Yang, Zhong‐Nan

doi:10.1111/j.1365-313x.2009.03929.x

Cited by 42 publications

(36 citation statements)

References 38 publications

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“…Moreover, since observations in meiotic spindle-defective mutants (e.g. mps1 and double heterozygous AtKIN14a/atkin14a AtKIN14b/atkin14b) have demonstrated that defects in MI or MII spindle structure do not affect postmeiotic cell plate formation (Quan et al, 2008;Jiang et al, 2009), we can exclude the possibility that cold-induced defects in postmeiotic RMA formation originate from slight (or maybe undetected) aberrations in MII spindle morphology. On the other hand, we cannot exclude the possibility that some cold-induced meiotic restitution events on rare occasions may directly originate from aberrations in spindle positioning, as already observed in heat-stressed rose meiocytes (e.g.…”

Section: Cold-induced Cytokinetic Defects In Meiosis Are Not Regulatementioning

confidence: 99%

Production of Diploid Male Gametes in Arabidopsis by Cold-Induced Destabilization of Postmeiotic Radial Microtubule Arrays

2012

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Whole-genome duplication through the formation of diploid gametes is a major route for polyploidization, speciation, and diversification in plants. The prevalence of polyploids in adverse climates led us to hypothesize that abiotic stress conditions can induce or stimulate diploid gamete production. In this study, we show that short periods of cold stress induce the production of diploid and polyploid pollen in Arabidopsis (Arabidopsis thaliana). Using a combination of cytological and genetic analyses, we demonstrate that cold stress alters the formation of radial microtubule arrays at telophase II and consequently leads to defects in postmeiotic cytokinesis and cell wall formation. As a result, cold-stressed male meiosis generates triads, dyads, and monads that contain binuclear and polynuclear microspores. Fusion of nuclei in binuclear and polynuclear microspores occurs spontaneously before pollen mitosis I and eventually leads to the formation of diploid and polyploid pollen grains. Using segregation analyses, we also found that the majority of cold-induced dyads and triads are genetically equivalent to a second division restitution and produce diploid gametes that are highly homozygous. In a broader perspective, these findings offer insights into the fundamental mechanisms that regulate male gametogenesis in plants and demonstrate that their sensitivity to environmental stress has evolutionary significance and agronomic relevance in terms of polyploidization.

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Section: Cold-induced Cytokinetic Defects In Meiosis Are Not Regulatementioning

confidence: 99%

Production of Diploid Male Gametes in Arabidopsis by Cold-Induced Destabilization of Postmeiotic Radial Microtubule Arrays

2012

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“…During advanced cytokinesis, the structure of the phragmoplast microtubules maintains the microspore nuclear distance and positioning (Hogan, 1987). Extensive studies have characterized the mechanism of dyad formation and have found that parallel spindle orientation is the most common cause of dyads (Bretagnolle and Thompson, 1995;Genualdo et al, 1998;Consiglio et al, 2004;Jiang et al, 2009).…”

Section: Afh14 Plays An Important Role In Cell Divisionmentioning

confidence: 99%

“…Preparation of PMCs for immunofluorescence microscopy was performed by as previously described (Jiang et al, 2009). Briefly, inflorescences were fixed in methanol:acetone (4:1, v/v) for 3 h, waving at 100g, and washed two times with PEM buffer.…”

Section: Staining and Immunolabeling For Fluorescence Microscopymentioning

confidence: 99%

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

et al. 2010

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Formins have long been known to regulate microfilaments but have also recently been shown to associate with microtubules. In this study, Arabidopsis thaliana FORMIN14 (AFH14), a type II formin, was found to regulate both microtubule and microfilament arrays. AFH14 expressed in BY-2 cells was shown to decorate preprophase bands, spindles, and phragmoplasts and to induce coalignment of microtubules with microfilaments. These effects perturbed the process of cell division. Localization of AFH14 to microtubule-based structures was confirmed in Arabidopsis suspension cells. Knockdown of AFH14 in mitotic cells altered interactions between microtubules and microfilaments, resulting in the formation of an abnormal mitotic apparatus. In Arabidopsis afh14 T-DNA insertion mutants, microtubule arrays displayed abnormalities during the meiosis-associated process of microspore formation, which corresponded to altered phenotypes during tetrad formation. In vitro biochemical experiments showed that AFH14 bound directly to either microtubules or microfilaments and that the FH2 domain was essential for cytoskeleton binding and bundling. However, in the presence of both microtubules and microfilaments, AFH14 promoted interactions between microtubules and microfilaments. These results demonstrate that AFH14 is a unique plant formin that functions as a linking protein between microtubules and microfilaments and thus plays important roles in the process of plant cell division.

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“…By contrast, only a few SAC proteins have been found to be involved in plant meiosis, including the Arabidopsis MPS1 and Aurora kinases (Jiang et al, 2009;Demidov et al, 2014) and the rice (Oryza sativa) Bub1-Related Kinase (BRK1; M. . CDC20 is a cofactor for both SAC and APC/C and has multiple roles in meiotic chromosome segregation in mice, Drosophila melanogaster, and bovine oocytes (Chu et al, 2001;Jin et al, 2010;Sun and Kim, 2012;Yang et al, 2014); however, the role of CDC20 in plant meiosis is unclear.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis Cell Division Cycle 20.1 Is Required for Normal Meiotic Spindle Assembly and Chromosome Segregation

et al. 2015

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Cell division requires proper spindle assembly; a surveillance pathway, the spindle assembly checkpoint (SAC), monitors whether the spindle is normal and correctly attached to kinetochores. The SAC proteins regulate mitotic chromosome segregation by affecting CDC20 (Cell Division Cycle 20) function. However, it is unclear whether CDC20 regulates meiotic spindle assembly and proper homolog segregation. Here, we show that the Arabidopsis thaliana CDC20.1 gene is indispensable for meiosis and male fertility. We demonstrate that cdc20.1 meiotic chromosomes align asynchronously and segregate unequally and the metaphase I spindle has aberrant morphology. Comparison of the distribution of meiotic stages at different time points between the wild type and cdc20

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MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled‐coil protein of Arabidopsis thaliana, is required for meiotic spindle organization

Cited by 42 publications

References 38 publications

Production of Diploid Male Gametes in Arabidopsis by Cold-Induced Destabilization of Postmeiotic Radial Microtubule Arrays

Production of Diploid Male Gametes in Arabidopsis by Cold-Induced Destabilization of Postmeiotic Radial Microtubule Arrays

The Type IIArabidopsisFormin14 Interacts with Microtubules and Microfilaments to Regulate Cell Division

Arabidopsis Cell Division Cycle 20.1 Is Required for Normal Meiotic Spindle Assembly and Chromosome Segregation

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