Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts

Mendes, Adriano A.; Castro, Heizir F. de; Rodrigues, D. de S.; Adriano, Wellington Sabino; Tardioli, Paulo Waldir; Mammarella, E; Giordano, Roberto de Campos; Giordano, Raquel de Lima Camargo

doi:10.1007/s10295-010-0880-9

Cited by 88 publications

(63 citation statements)

References 40 publications

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“…21 Os hidrogéis híbridos de quitosana--alginato contendo leveduras (S. cerevisiae) coimobilizadas na concentração de 10% m/m seguido de ruptura celular com sabão em pó foram preparados conforme metodologia descrita por Adriano et al. 19 Os hidrogéis obtidos foram lavados com água destilada, filtrados a vácuo e estocados sob refrigeração.…”

Section: Preparação Dos Hidrogéis Híbridos Quitosana-alginatounclassified

“…21 Hidrogel híbrido quitosana-alginato modificado quimicamente com laurinaldeído foi preparado empregando diferentes concentrações do agente (1, 3 e 5% m/m). A reação de modificação química foi realizada em tampão fosfato de sódio pH 7,0 (200 mM) por 1 h a 25 °C.…”

Section: Modificação Química Dos Hidrogéis Híbridos Quitosana-alginatunclassified

“…21 Os hidrogéis híbridos ativados foram lavados com água destilada, filtrados a vácuo e estocados sob refrigeração.…”

Section: Ativação De Hidrogéis Híbridos Quitosana-alginato-tnbs Com Gunclassified

“…A imobilização de enzimas em suportes com elevada concentração de grupos glioxil (grupos aldeído) permite a obtenção de derivados termoestáveis decorrentes da intensa interação dos resíduos lisina da enzima com os grupos glioxil do suporte, com a formação de bases de Schiff (-C=N-). 12,[17][18][19]21 Esta estabilização é devido à boa congruência geométrica de alguns suportes, como glioxil-agarose, que possibilita um correto alinhamento entre a enzima e o suporte, contribuindo para a formação de multi-interações que aumentam significativamente a estabilidade térmica dos derivados preparados. 12,17,18 Muitas enzimas têm sido estabilizadas em suportes glioxil, com atividade recuperada variando de 60 a 100% e elevada estabilização térmica, na maioria dos casos superiores a 10 3 e, em certos casos, estes valores são superiores a 10 5 .…”

Section: Introductionunclassified

“…Esta lipase tem sido amplamente empregada em reações em meio orgânico e aquoso para a obtenção de produtos de grande interesse industrial, como concentrados de ácidos graxos livres por hidrólise seletiva de diferentes óleos e gorduras, biodiesel, lipídeos estruturados, ésteres aromatizantes, formulação de detergentes, resolução de misturas racêmicas, degradação de polímeros e no pré-tratamento de efluentes com elevados teores de lipídeos. 5,[21][22][23] A lipase foi imobilizada por ligação covalente multipontual em bagaço de cana-de-açúcar, partículas de poli-hidróxibutirato (PHB) e hidrogéis híbridos de quitosana-alginato. Com o propósito de aumentar o tamanho de poros dos hidrogéis híbridos, foi adotada a técnica de coimobilização de células de leveduras (Saccharomyces cerevisiae), seguida de ruptura celular.…”

Section: Introductionunclassified

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Triagem de suportes orgânicos e protocolos de ativação na imobilização e estabilização de lipase de Thermomyces lanuginosus

Mendes¹,

Castro²,

Giordano³

2013

Quím. Nova

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Thermomyces lanuginosus. Lipase from Thermomyces lanuginosus was covalently immobilized on activated poly-hydroxybutyrate, sugarcane bagasse and the chemically modified hybrid hydrogel chitosan-alginate prepared by different strategies. Among the tested supports, chitosan-alginate chemically modified with 2,4,6-trinitrobenzenesulfonic acid rendered derivatives with the highest hydrolytic activity and thermal-stability, 45-fold more stable than soluble lipase and was then selected for further studies. The pH of maximum activity was similar for both immobilized and free lipase (pH 8.0) while optimum temperature was 5 -10 ºC higher for the immobilized lipase. Higher yields in the butyl butyrate synthesis were found for the derivatives prepared by activation with glycidol and epichlorohydrin.

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Section: Preparação Dos Hidrogéis Híbridos Quitosana-alginatounclassified

Section: Modificação Química Dos Hidrogéis Híbridos Quitosana-alginatunclassified

“…21 Os hidrogéis híbridos ativados foram lavados com água destilada, filtrados a vácuo e estocados sob refrigeração.…”

Section: Ativação De Hidrogéis Híbridos Quitosana-alginato-tnbs Com Gunclassified

Section: Introductionunclassified

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Triagem de suportes orgânicos e protocolos de ativação na imobilização e estabilização de lipase de Thermomyces lanuginosus

Mendes¹,

Castro²,

Giordano³

2013

Quím. Nova

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Recent advances on sources and industrial applications of lipases

Sarmah

Revathi

Sheelu

et al. 2017

Biotechnology Progress

270

123

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Lipases are the industrially important biocatalysts, which are envisioned to have tremendous applications in the manufacture of a wide range of products. Their unique properties such as better stability, selectivity and substrate specificity position them as the most expansively used industrial enzymes. The research on production and applications of lipases is ever growing and there exists a need to have a latest review on the research findings of lipases. The present review aims at giving the latest and broadest overall picture of research and development on lipases by including the current studies and progressions not only in the diverse industrial application fields of lipases, but also with regard to its structure, classification and sources. Also, a special emphasis has been made on the aspects such as process optimization, modeling, and design that are very critical for further scale-up and industrial implementation. The detailed tabulations provided in each section, which are prepared by the exhaustive review of current literature covering the various aspects of lipase including its production and applications along with example case studies, will serve as the comprehensive source of current advancements in lipase research. This review will be very useful for the researchers from both industry as well as academia in promoting lipolysis as the most promising approaches to intensified, greener and sustainable processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:5-28, 2018.

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1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates

Ramos

Miranda

Giordano

et al. 2018

Biotechnology Progress

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The preparation of crosslinked aggregates of pancreatic porcine lipase (PPL-CLEA) was systematically studied, evaluating the influence of three precipitants and two crosslinking agents, as well as the use of soy protein as an alternative feeder protein on the catalytic properties and stability of the immobilized PPL. Standard CLEAs showed a global yield (CLEA' observed activity/offered total activity) of less than 4%, whereas with the addition of soy protein (PPL:soy protein mass ratio of 1:3) the global yield was approximately fivefold higher. The CLEA of PPL prepared with soy protein as feeder (PPL:soy protein mass ratio of 1:3) and glutaraldehyde as crosslinking reagent (10 μmol of aldehyde groups/mg of total protein) was more active mainly because of the reduced enzyme leaching in the washing step. This CLEA, named PPL-SOY-CLEA, had an immobilization yield around 60% and an expressed activity around 40%. In the ethanolysis of soybean oil, the PPL-SOY-CLEA yielded maximum fatty acid ethyl ester (FAEE) concentration around 12-fold higher than that achieved using soluble PPL (34 h reaction at 30°C, 300 rpm stirring, soybean oil/ethanol molar ratio of 1:5) with an enzyme load around 2-fold lower (very likely due to free enzyme inactivation). The operational stability of the PPL-SOY-CLEA in the ethanolysis of soybean oil in a vortex flow type reactor showed that FAEE yield was higher than 50% during ten reaction cycles of 24 h. This reactor configuration may be an attractive alternative to the conventional stirred reactors for biotransformations in industrial plants using carrier-free biocatalysts. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:910-920, 2018.

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Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts

Cited by 88 publications

References 40 publications

Triagem de suportes orgânicos e protocolos de ativação na imobilização e estabilização de lipase de Thermomyces lanuginosus

Triagem de suportes orgânicos e protocolos de ativação na imobilização e estabilização de lipase de Thermomyces lanuginosus

Recent advances on sources and industrial applications of lipases

1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates

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