Multiplication of Orally Administered<i>Clostridium butyricum</i>in Rats

Sato, Rumiko; Tanaka, Mamoru

doi:10.3109/08910609609166451

Cited by 10 publications

(14 citation statements)

References 28 publications

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“…High amylose maize starch (HI-MAIZE TM GELOSE91, 70% amylose), kindly supplied by PENFORD (Australia), was added to the normal diet in a ratio of 1:5 and was fed to the rats as the HAS diet. C. butyricum MIYAIRI588 (ϭCBM588) spores were purified by density gradient centrifugation (37). CBM588 colonies grown on Nutrient agar (Nissui Pharmaceutical Co., Ltd., Tokyo) containing 0.5% glucose and 0.3% CaCO 3 were suspended in sterile water.…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of intestinal microbiota was performed according to the method reported by Mitsuoka (28). Serial dilutions of colonic contents were plated on nonselective media (BL and TS agar) and selective media (BS, NBGT, DHL, TATAC and LBS agar) and on a C. butyricum-selective medium (37) in an anaerobic chamber (ANX-1, Hirasawa, Tokyo). The plates were incubated at 37 C for one to three days in aerobic or anaerobic conditions.…”

Section: Methodsmentioning

confidence: 99%

“…C. butyricum produces SCFA such as butyrate and acetate and has been used as a probiotic in humans and animals (19,23,42). C. butyricum spores orally administered to rats can germinate and grow in the intestinal tracts (37). C. butyricum can utilize amylose and produce large amounts of butyrate and acetate (unpublished data).…”

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confidence: 99%

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Effects of High Amylose Maize Starch and Clostridium butyricum on Metabolism in Colonic Microbiota and Formation of Azoxymethane‐Induced Aberrant Crypt Foci in the Rat Colon

Nakanishi

Kataoka

Kuwahara

et al. 2003

Microbiology and Immunology

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High amylose maize starch (HAS) is not digested in the small intestine and most of it reaches the large intestine. In the large intestine, HAS is fermented by intestinal bacteria, resulting in production of short‐chain fatty acids (SCFA), particularly butyrate. Clostridium butyricum can utilize HAS and produce butyrate and acetate. It has been proposed that butyrate inhibits carcinogenesis in the colon. In this study, we examined the inhibitory effects of HAS and C. butyricum strain MIYAIRI588 (CBM588) on azoxymethane‐induced aberrant crypt foci (ACF) formation in rats. In the group of rats administered only CBM588 spores, the concentration of butyrate in the cecum increased, but there was no decrease in the number of ACF. In the group of rats fed an HAS diet, a decrease in the number of ACF was observed, and in the group of rats administered HAS and CBM588, the number of ACF decreased significantly. In these two groups, the concentrations of acetate and propionate in intestinal contents significantly increased, but the concentration of butyrate did not change. It was found that the β‐glucuronidase activity level of colonic contents decreased significantly in the two groups of rats fed HAS. This study showed that HAS and CBM588 changed the metabolism of colonic microbiota and decreased the level of β‐glucuronidase activity, phenomena that may play a role in the inhibition of ACF formation in the rat colon.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of High Amylose Maize Starch and Clostridium butyricum on Metabolism in Colonic Microbiota and Formation of Azoxymethane‐Induced Aberrant Crypt Foci in the Rat Colon

Nakanishi

Kataoka

Kuwahara

et al. 2003

Microbiology and Immunology

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“…CBM588 is a Gram-positive rod-shaped obligate anaerobe and spore-forming bacterium [95]. Unlike pathogenic clostridia, such as Clostridium difficile, Clostridium perfringens, and Clostridium botulinum, CBM588 is nonpathogenic and has been used for treatment of human gastrointestinal disease in clinical settings [96,97].…”

Section: Clostridium Butyricum Induces Il-10-producing Macrophagesmentioning

confidence: 99%

A breakthrough in probiotics: Clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease

2015

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Intestinal immune homeostasis is regulated by gut microbiota, including beneficial and pathogenic microorganisms. Imbalance in gut bacterial constituents provokes host proinflammatory responses causing diseases such as inflammatory bowel disease (IBD). The development of next-generation sequencing technology allows the identification of microbiota alterations in IBD. Several studies have shown reduced diversity in the gut microbiota of patients with IBD. Advances in gnotobiotic technology have made possible analysis of the role of specific bacterial strains in immune cells in the intestine. Using these techniques, we have shown that Clostridium butyricum as a probiotic induces interleukin-10-producing macrophages in inflamed mucosa via the Toll-like receptor 2/myeloid differentiation primary response gene 88 pathway to prevent acute experimental colitis. In this review, we focus on the new approaches for the role of specific bacterial strains in immunological responses, as well as the potential of bacterial therapy for IBD treatments.

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“…In addition, our previous study demonstrated that butyric acid has the specific property of inhibiting the survival of H. pylori independent of low pH (Takahashi et al, 2000). Furthermore, the cell-free supernatant of Clostridium butyricum MIYAIRI 588, a butyric acid-producing probiotic bacterium commonly used for treating and preventing both non-antimicrobial-induced and antimicrobial-associated diarrhoea in humans and animals (Sato & Tanaka, 1997;Kamiya et al, 1997), also exhibited antibacterial properties independent of the decrease in pH (Takahashi et al, 2000). These observations suggest that the butyric acid produced by P. gingivalis could be an antibacterial agent against H. pylori in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

Destructive effects of butyrate on the cell envelope of Helicobacter pylori

Yonezawa

Osaki

Hanawa

et al. 2012

Journal of Medical Microbiology

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Helicobacter pylori can be found in the oral cavity and is mostly detected by the use of PCR techniques. Growth of H. pylori is influenced by various factors in the mouth, such as the oral microflora, saliva and other antimicrobial substances, all of which make colonization of the oral cavity by H. pylori difficult. In the present study, we analysed the effect of the cell supernatant of a representative periodontal bacterium Porphyromonas gingivalis on H. pylori and found that the cell supernatant destroyed the H. pylori cell envelope. As P. gingivalis produces butyric acid, we focused our research on the effects of butyrate and found that it significantly inhibited the growth of H. pylori. H. pylori cytoplasmic proteins and DNA were detected in the extracellular environment after treatment with butyrate, suggesting that the integrity of the cell envelope was compromised and indicating that butyrate has a bactericidal effect on H. pylori. In addition, levels of extracellular H. pylori DNA increased following treatment with the cell supernatant of butyric acid-producing bacteria, indicating that the cell supernatant also has a bactericidal effect and that this may be due to its butyric acid content. In conclusion, butyric acid-producing bacteria may play a role in affecting H. pylori colonization of the oral cavity.

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Multiplication of Orally AdministeredClostridium butyricumin Rats

Cited by 10 publications

References 28 publications

Effects of High Amylose Maize Starch and Clostridium butyricum on Metabolism in Colonic Microbiota and Formation of Azoxymethane‐Induced Aberrant Crypt Foci in the Rat Colon

Effects of High Amylose Maize Starch and Clostridium butyricum on Metabolism in Colonic Microbiota and Formation of Azoxymethane‐Induced Aberrant Crypt Foci in the Rat Colon

A breakthrough in probiotics: Clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease

Destructive effects of butyrate on the cell envelope of Helicobacter pylori

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