Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine

Aitken-Christie, J.; Singh, Adya P.; Davies, Helen

doi:10.1007/978-1-4613-1661-9_26

Cited by 103 publications

(51 citation statements)

References 21 publications

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“…Large-scale liquid culture of plants regenerating via organogenesis is rather limited and has been reported for ferns (Ziv & Hadar 1991), Philodendron (Ziv & Ariel 1991), Begonia, Lilium (Takayama & Misawa 1983;Takayama et al 1990) and Gladiolus (Ziv 1989(Ziv , 1990a(Ziv , b, 1992b. In poplar and Pinus, organ development in liquid or agar cultures was initiated from highly meristematic nodular cluster tissues (McCown et al 1988;Aitken-Christie et al 1988). A 500 1 capacity bioreactor was used by Takahashi et al (1992) to propagate Lilium bulblets.…”

Section: Introductionmentioning

confidence: 99%

Scaled-up proliferation and regeneration of Nerine in liquid cultures Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors

Ziv

Kahany

Lilien-Kipnis

1994

Plant Cell Tiss Organ Cult

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The natural propagation rate of bulb forming Amaryllidaceae including Nerine is low. Conventional micropropagation techniques are labor intensive and therefore expensive. Liquid cultures facilitate: scaling up, automation and cost reduction of micropropagation. Inflorescence-derived explants of Nerine were cultured on 2,4-dichlorophenoxyacetic acid (2,4-9) and 6-benzyladenine (BA) supplemented Murashige & Skoog (MS) medium. Callus-like tissue interspersed with nodular tissue, as well as direct organogenesis developed at the junction between flower pedicel and peduncle. Subculture of nodular tissue to 1-naphthalene acetic acid (NAA), BA and paclobutrazol (PAC) supplemented liquid medium in Erlenmeyer flasks or bubble bioreactors resulted in proliferation of rounded, compact, easily crumbled meristematic clusters. Growth and proliferation in bioreactors were higher than in shaken flasks and were affected differently by the inoculum to medium ratio in the two types of culture vessel. Nerine cultures showed low sensitivity to high aeration rates in bubble bioreactors despite the accumulation of debris. It was therefore possible to increase aeration rates without reducing the proliferation rate or damaging the quality of the meristematic aggregates. The conditions in semi-continuous culture in flasks and bioreactors were more favorable and increased the growth value by 100% and 140%, respectively. The total protein content increased by 180% in flasks and 90% in bioreactors. Although the presence of PAC throughout the culture period decreased growth and proliferation, it was a promotive bioregulator for meristernatic cluster formation. Proembryogenic clusters developed upon the removal of PAC. The use of meristematic clusters for micropropagation in scaled-up liquid cultures is discussed.

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Section: Introductionmentioning

confidence: 99%

Scaled-up proliferation and regeneration of Nerine in liquid cultures Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors

Ziv

Kahany

Lilien-Kipnis

1994

Plant Cell Tiss Organ Cult

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“…The extension of this approach to produce transformed plants depends upon the successful regeneration of plants from transformed cells or tissues. Several tissue culture systems have potential for this approach, including the regeneration of plants from cotyledons [21], from somatic embryos [11,12], from callus [9], or from proliferating nodules [ 1 ]. These and other conifer tissue culture techniques have been reviewed by Dunstan [6].…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated DNA transfer in sugar pine

1990

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DNA transfer using Agrobacterium tumefaciens has been demonstrated in sugar pine, Pinus lambertiana Dougl. Shoots derived from cytokinin-treated cotyledons formed galls after inoculation with A. tumefaciens strains containing the plasmid pTiBo542. A selectable marker, neomycin phosphotransferase II, conferring resistance to kanamycin, was transferred into sugar pine using a binary armed vector system. Callus proliferated from the galls grew without hormones and in some cases, kanamycin-resistant callus could be cultured. Southern blots provided evidence of physical transfer of T-DNA and the nptII gene. Expression of the nptII gene under control of the nos promoter was demonstrated by neomycin phosphotransferase assays. Several aspects of DNA transfer were similar to those previously observed in angiosperms transformed by A. tumefaciens. This is the first evidence for DNA transfer by Agrobacterium in this species and the first physical evidence for transfer in any pine. These results bring us closer to genetic engineering in this commercially important genus of forest trees.

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“…These shoots are subsequently rooted to form plantlets. Although organogene-sis has been very effective for some conifer species, in particular P. radiata (Aitken-Christie et al 1988;Montalbán et al 2011), for many, this technology never reached a practical application stage primarily because of low plantlet formation rates, poor rooting, and excessive handling and costs.…”

Section: Organogenesismentioning

confidence: 99%

A comparative evaluation of the application of somatic embryogenesis, rooting of cuttings, and organogenesis of conifers

Bonga

2015

Can. J. For. Res.

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Vegetative propagation of conifers has found large-scale industrial application via somatic embryogenesis (SE), rooting of cuttings, and organogenesis. Genetic gain is achieved with all of these methods but is the highest with SE, primarily because SE cultures can be cryopreserved. This allows for plants derived from part of each cell line to be field tested over a long period while the rest of each cell line is kept in a juvenile state by cryopreservation for later use. This makes it possible to select the best performers within the best families. For rooting of cuttings and organogenesis, genetic gain is generally based on family average, which is less powerful. However, SE has its limitations, primarily because its initiation, maturation, or germination rates are too low to be effective for many species. Consequently, for many species, the preferred clonal propagation option is still rooting of cuttings or organogenesis. If better methods can be developed to keep ortets used for rooting of cuttings and organogenesis in a prolonged juvenile state, or if future developments in marker technology reach a point where within-family selection becomes possible without the aid of cryopreservation, rooting of cuttings and organogenesis will achieve the same genetic gain as SE.Key words: cryopreservation, genetic gain, juvenility, ortet, ramet.Résumé : La propagation végétative des conifères a trouvé une application industrielle à grande échelle via l'embryogenèse somatique (ES), l'enracinement de boutures et l'organogenèse. Toutes ces méthodes permettent d'obtenir un gain génétique mais l'ES procure le gain maximum, surtout parce que les cultures d'ES peuvent être congelées. Cela permet de tester au champ sur une longue période les plants dérivés d'une partie de chaque lignée cellulaire tandis que le reste de chaque lignée est conservé dans un état juvénile par cryoconservation pour usage ultérieur. De cette façon il est possible de sélectionner les plants qui performent le mieux au sein des meilleures familles. Dans le cas des boutures racinées et de l'organogenèse, le gain génétique est généralement basé sur la moyenne de la famille, ce qui est moins performant. Cependant, l'ES a ses limites, principalement parce qu'avec plusieurs espèces l'initiation, la maturation ou le taux de germination sont trop lents pour être efficaces. Par conséquent, dans le cas de plusieurs espèces l'option de propagation clonale préférée demeure l'enracinement de boutures ou l'organogenèse. Si on arrive à développer des méthodes pour conserver dans un état juvénile prolongé les ortets utilisés pour l'enracinement de boutures ou l'organogenèse, ou si les progrès dans la technologie des marqueurs atteignent un point où la sélection intrafamiliale devient possible sans avoir recours à la cryoconservation, l'enracinement de boutures et l'organogenèse vont procurer le même gain génétique que l'ES. [Traduit par la Rédaction]

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Multiplication of Meristematic Tissue: A New Tissue Culture System for Radiata Pine

Cited by 103 publications

References 21 publications

Scaled-up proliferation and regeneration of Nerine in liquid cultures Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors

Scaled-up proliferation and regeneration of Nerine in liquid cultures Part I. The induction and maintenance of proliferating meristematic clusters by paclobutrazol in bioreactors

Agrobacterium-mediated DNA transfer in sugar pine

A comparative evaluation of the application of somatic embryogenesis, rooting of cuttings, and organogenesis of conifers

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