Multiplication of measles virus in cell cultures.

Matumoto, M

doi:10.1128/mmbr.30.1.152-176.1966

Cited by 22 publications

(9 citation statements)

References 70 publications

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“…Both facts suggest a difference in growth pattern between SSPE virus and measles virus in culture that should be examined further. The other growth properties, such as thermolability of the SSPE virus and the lack of effect of actinomycin D on virus production, are similar to that reported for measles virus (19).…”

Section: Discussionsupporting

confidence: 77%

“…The growth properties of the LEC strain SSPE virus in CV-1 cells differ from the in vitro growth properties of measles virus in two respects. First, the SSPE virus-infected cells undergo cytopathic changes culminating in their destruction within 48 hr after infection, whereas measles virus-infected cells were able to maintain cell integrity for a much longer time (19,22). Secondly, the titer of extracellular virus of SSPE-infected cultures never attains the high infections titer attained by measles virus infections (19,22).…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

Yeh

Iwasaki

1972

J Virol

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Nucleocapsids from subacute sclerosing panencephalitis (SSPE) virus-infected CV-1 cells were concentrated by differential centrifugation employing sucrose cushion techniques and further purified by centrifugation through a linear CsCl density gradient. The bouyant density of 3H-uridine-labeled nucleocapsids in CsCl was found to be 1.31 g/cm3. Ultraviolet absorption spectra of the purified SSPE nucleocapsid showed an absorption maximum at 260 to 265 nm and a 280/260 ratio that corresponded to a nucleic acid content of approximately 4.3 Negatively stained preparations of SSPE nucleocapsids were found to have a width of

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

Yeh

Iwasaki

1972

J Virol

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“…Shortly thereafter, it was reported that the virus could also replicate in established cell lines and chicken embryos (100,231), and plaque assay systems were developed (152,371). It has subsequently been shown that measles virus can be grown in a variety of primary and continuous cell lines, although the best results are obtained with cells of human and simian origin (225).…”

Section: Virus-cell Interactionsmentioning

confidence: 99%

Measles virus and its associated diseases.

Morgan¹,

Rapp²

1977

Bacteriological Reviews

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“…The availability of systems for growing relatively large amounts of some paramyxoviruses [Newcastle disease virus (NDV), Sendai virus, and simian virsus 5] in large part accounts for the fact that most of what is presently known about paramyxovirus structure and replication comes from work with these viruses (1,18,27). The much poorer growth, especially in cell culture systems, of paramyxoviruses more pertinent to human disease probably explains the paucity of data on their structure and replication (6,21). It is important to learn more about these neglected paramyxoviruses not only because of their relevance to medicine but because the biological significance of phenomena like the synthesis of single-stranded ribonucleic acid (RNA) species complementary to virus genomes in infected cells (2,3,16) "incomplete" or defective visions containing viral genome fragments (20) may not be fully understood until it is known how general they are among the paramyxovirus group.…”

mentioning

confidence: 99%

Mumps Virus Replication in Chick Embryo Lung Cells: Properties of Ribonucleic Acids in Virions and Infected Cells

East

Kingsbury

1971

J Virol

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Ribonucleic acid (RNA) species in mumps virions and in infected cells were compared. The predominant RNA species in virions labeled with 3 H-uridine sedimented at 50 S ; RNA species sedimenting at 28, 18, and about 10 S were also present. The virion-associated RNA species sedimenting slower than 50 S contained some nucleotide sequences similar to 50 S virion RNA. Although mumps virus replication was severely inhibited by high concentrations of actinomycin D, some virus was made, and virus-specific RNA species accumulated in infected cells. Mumps virus resembled other paramyxoviruses in inducing, in infected cells, synthesis not only of 50 S RNA but also of slower sedimenting RNA species with a peak distribution at about 18 S , complementary in base sequences to 50 S virion RNA. In addition, base sequences of the parental type were relatively abundant in the RNA species sedimenting slower than 50 S ; these may represent precursors of the slowly sedimenting RNA species associated with virions. Ribonuclease-resistant RNA was detected in infected cells; this may represent replicative or transcriptive intermediates. Inhibition of protein synthesis with cycloheximide severely depressed accumulation of labeled 50 S RNA in infected cells but did not interfere with accumulation of RNA species sedimenting slower than 50 S . Actinomycin D treatment had a similar effect. Annealing of genomes and virus-induced complementary RNA species of Newcastle disease virus, Sendai virus, and mumps virus did not reveal any base sequence homologies.

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Multiplication of measles virus in cell cultures.

Cited by 22 publications

References 70 publications

Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

Measles virus and its associated diseases.

Mumps Virus Replication in Chick Embryo Lung Cells: Properties of Ribonucleic Acids in Virions and Infected Cells

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