Multiplication of fluorescent pseudomonads at low substrate concentrations in tap water

Abstract

Two fluorescent pseudomonads, strains P17 and P500, belonging to different biotypes were tested for growth in tap water supplied with different concentration of acetate and glutamate, low concentrations (10 and 20 micrograms of C per liter) of various other substrates and mixtures of related substrates, the latter being present in amounts of 1 microgram of C per liter each. Amino acids appeared to be excellent substrates for both isolates, but many other substrates were utilized at very low concentrations as well. Saturation constants (Ks) of P17 with acetate, arginine, aspartate, glutamate, lactate, succinate, malonate, p-hydroxybenzoate and glucose were all below 1 microM. The Ks values of strain P500 were about 5 times larger than those of P17. Since especially P17 is able to use a large number of different substrates at low concentrations, assessment of maximal colony counts of this organism by growth experiments in various types of tap water may give information about the concentrations of easily assimilable organic carbon.

Flavobacterium johnsoniae as a Model Organism for Characterizing Biopolymer Utilization in Oligotrophic Freshwater Environments

“…However, knowledge of nutritional versatility and growth kinetics is still essential for determining the role of these bacteria in the degradation of specific organic compounds in the environment. In contrast to the amount of kinetic data available in literature on oligotrophic aquatic bacteria consuming LMW compounds (9,10,27,42,48,52), information on kinetic parameters of oligotrophic aquatic bacteria consuming HMW compounds is scarce.In a recent study, Flavobacterium johnsoniae strain A3 was isolated from tap water supplemented with 100 g of polysaccharide carbon liter Ϫ1 and a river water inoculum (40). Strain A3 is particularly proficient in the utilization of a diverse group of oligo-and polysaccharides at g liter Ϫ1 levels in natural and treated water.…”

“…However, knowledge of nutritional versatility and growth kinetics is still essential for determining the role of these bacteria in the degradation of specific organic compounds in the environment. In contrast to the amount of kinetic data available in literature on oligotrophic aquatic bacteria consuming LMW compounds (9,10,27,42,48,52), information on kinetic parameters of oligotrophic aquatic bacteria consuming HMW compounds is scarce.…”

“…Indeed, heterotrophic bacteria can utilize multiple LMW energy and carbon sources simultaneously and at lower threshold concentrations than during growth with single substrates (21,27,29). Furthermore, a variety of heterotrophic bacteria (including strain A3) can utilize LMW organic compounds that individually do not serve as the energy source when growth-promoting organic compounds are present (40,47,50,52). The growth kinetic parameters of strain A3 with biopolymers have been determined only in the presence of a few g liter Ϫ1 of indigenous substrates in the blank and not in the presence of other added substrates.…”

Respiratory characteristics of two oligotrophic bacteria: Agromonas oligotrophica JCM 1494 and Aeromonas hydrophila 315.

“…In the past decade, several known bacterial species were characterized as oligotrophs and novel types of these were isolated from natural environments, revealing their diversity and wide distribution. Some of the striking observations concerning new types of oligotrophs were reported by FAVERO et al (11) and VAN DER KooIJ et al (12)(13)(14). They observed the growth of opportunistic pathogens such as Pseudomonas aeruginosa, P.…”

Polysaccharides and Proteins Added to Flowing Drinking Water at Microgram-per-Liter Levels Promote the Formation of Biofilms Predominated by Bacteroidetes and Proteobacteria

“…in batch tests (23,24,44,45). These findings are consistent with previously observed differences in growth kinetics between biofouling and planktonic growth with acetate at g C liter Ϫ1 levels (46).…”