Multiplication and Distribution of Sugarcane White Leaf Phytoplasma Transmitted by the Leafhopper, Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae), in Infected Sugarcane

Roddee, Jariya; Kobori, Youichi; Hanboonsong, Yupa

doi:10.1007/s12355-017-0559-x

Cited by 21 publications

(18 citation statements)

References 16 publications

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“…() and Roddee et al. (). Uninfected 5‐ to 7‐day‐old post‐emergence male and female adult insects were used in the following experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The qPCR reactions used 5 μl of 1× SYBR Green Supermix, 0.4 μl of each specific primer (10 μ m ), 1 μl of nucleic acid extracts, and sterile water to adjust the final volume to 10 μl, as described by Roddee et al. (). The qPCR was performed in 48‐well plates using a Bio‐Rad iCycler (Bio‐Rad Laboratories, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Ten‐fold serial dilutions were made in 1× TE buffer (pH 8.0), with plasmid DNA concentrations ranging from 3 × 10 7 to 3 × 10 2 copies μl −1 as described by Roddee et al. ().…”

Section: Methodsmentioning

confidence: 99%

“…Insect colonies were maintained in a controlled greenhouse at 28 AE 2°C and 70-80% r.h., under natural light. Each insect generation was collected and tested for SCWL phytoplasma infection by quantitative polymerase chain reaction (qPCR) using specific primers, as described by Hanboonsong et al (2002) and Roddee et al (2018). Uninfected 5-to 7-dayold post-emergence male and female adult insects were used in the following experiments.…”

Section: Insect Coloniesmentioning

confidence: 99%

“…The electrical penetration graph (EPG) technique has been used to study insect feeding behaviour linked to the transmission process of pathogens (Tjallingii, 1985;Jacobson & Kennedy, 2014;Luo et al, 2015;Wu et al, 2016). In our previous study, we described six EPG waveforms correlated with the stylet penetration activities during probing by the SCWL phytoplasma vector M. hiroglyphicus, of which the waveforms C and D were linked to acquisition and inoculation, respectively (Roddee et al, 2018). However, no information about the stylet penetration activities of M. hiroglyphicus related to SCWL phytoplasma transmission is available.…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Roddee

Kobori

Hanboonsong

2018

Entomologia Exp Applicata

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The leafhopper Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae) is the most important vector of sugarcane white leaf (SCWL) phytoplasma that significantly affects the sugarcane crop in Asia. Here, we aimed to study the characteristics of SCWL phytoplasma transmission by M. hiroglyphicus. To this end, the stylet penetration activities performed during the acquisition access period (AAP) and inoculation access period (IAP) were investigated by the direct current electrical penetration graph technique and confirmed by quantitative polymerase chain reaction (qPCR). Additionally, the latent period (LP) of SCWL phytoplasma in the vector was determined by qPCR and localised by fluorescent in situ hybridisation. The results indicated that the acquisition of SCWL phytoplasma occurred during phloem ingestion (waveform D), whereas its inoculation was associated with salivation into the phloem sieve element (waveform C). The minimum AAP was 15 min and the minimum duration of phloem ingestion was 2.35 min. The minimum LP of SCWL phytoplasma in the vector was at least 14 days; then, SCWL phytoplasma moved to the salivary glands of the insect, enabling the transmission of the pathogen to the host plants. The minimum IAP for a successful transmission of SCWL phytoplasma to the host plants was 11–12 min, with a minimum duration of salivation into phloem of 1.35 min. The female vectors had higher SCWL phytoplasma copy numbers than the male vectors, and displayed faster AAP, IAP, and LP. Overall, our findings provide important information related to the feeding behaviour of M. hiroglyphicus and its effect on the transmission of SCWL phytoplasma.

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“…() and Roddee et al. (). Uninfected 5‐ to 7‐day‐old post‐emergence male and female adult insects were used in the following experiments.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Ten‐fold serial dilutions were made in 1× TE buffer (pH 8.0), with plasmid DNA concentrations ranging from 3 × 10 7 to 3 × 10 2 copies μl −1 as described by Roddee et al. ().…”

Section: Methodsmentioning

confidence: 99%

Section: Insect Coloniesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Roddee

Kobori

Hanboonsong

2018

Entomologia Exp Applicata

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Genetic differentiation and phylogeography of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) in the southwestern karst area of China

Luo,

Pu,

Wang

et al. 2024

Ecology and Evolution

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Erythroneurini is the largest tribe of the microleafhopper subfamily Typhlocybinae. Most prior research on this tribe has focused on traditional classification, phylogeny, and control of agricultural pests, and the phylogeography of the group remains poorly understood. In this study, the mitochondrial genomes of 10 erythroneurine species were sequenced, and sequences of four genes were obtained for 12 geographical populations of Seriana bacilla. The new sequence data were combined with previously available mitochondrial DNA sequence data and analyzed using Bayesian and Maximum‐Likelihood‐based phylogenetic methods to elucidate relationships among genera and species and estimate divergence times. Seriana was shown to be derived from within Empoascanara. Phylogeographic and population genetic analysis of the endemic Chinese species Seriana bacilla suggest that the species diverged about 54.85 Mya (95% HPD: 20.76–66.23 million years) in the Paleogene period and that population divergence occurred within the last 14 million years. Ancestral area reconstruction indicates that Seriana bacilla may have originated in the central region of Guizhou, and geographical barriers are the main factors affecting gene flow among populations. Ecological niche modeling using the MaxEnt model suggests that the distribution of the species was more restricted in the past but is likely to expand in the future years 2050 and 2070.

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The role of insect intestinal microbes in controlling of Empoasca onukii Matsuda (Hemiptera: Cicadellidae) pest infestations in the production of tea garden: a review

2023

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Multiplication and Distribution of Sugarcane White Leaf Phytoplasma Transmitted by the Leafhopper, Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae), in Infected Sugarcane

Cited by 21 publications

References 16 publications

Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Characteristics of sugarcane white leaf phytoplasma transmission by the leafhopper Matsumuratettix hiroglyphicus

Genetic differentiation and phylogeography of Erythroneurini (Hemiptera, Cicadellidae, Typhlocybinae) in the southwestern karst area of China

The role of insect intestinal microbes in controlling of Empoasca onukii Matsuda (Hemiptera: Cicadellidae) pest infestations in the production of tea garden: a review

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