Multiplicación in vitro de Psidium guajava L. en sistemas de inmersión temporal

Vilchez, J.; Albany, N.

doi:10.15446/rev.colomb.biote.v16n2.42180

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…One of the methods of asexual propagation is through in vitro propagation, which has been successful due to its effectiveness and produces homogeneous material that preserves the characteristics of the mother plant (Vilchez & Albany, 2014). Additionally, through this technique, the behaviour of varieties exposed to different stressors controlled conditions can be evaluated, and it is thus possible to determine which ones are more suitable for different field conditions.…”

Section: Introductionmentioning

confidence: 99%

Responses of stevia (Stevia rebaudiana Bertoni) to salinity conditions

Piris,

Duré,

Samudio-Oggero

et al. 2023

Acta Agro

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The sweet herb or ka'a he'ẽ in the Guarani language (Stevia rebaudiana Bertoni) is a native plant of Paraguay, which produces a natural sweetener, 300 times sweeter than sucrose. The consumption of stevia has spread to numerous countries due to its beneficial properties for health. To meet the global demand for stevia, the production must be optimized through the use of improved varieties cultivated in different environmental conditions. This is an important fact, given the challenges related to climate change, such as salinity, opting for varieties that adapt to these conditions generates a positive impact on the dissemination of the crop. This research was aimed to determine the response of the Eirete variety of S. rebaudiana to saline stress conditions by evaluating selected biochemical and growth parameters. Vitroplants propagated in Murashige and Skoog culture medium (MS) supplemented with 0, 25, 50, 75, or 100 mM NaCl were used in the study to identify the concentration that reduced growth by 50% (GR50) and with 0, 50, 100, 150, and 200 mM NaCl to determine the concentration that caused the death of 50% of the individuals (LD50). After the evaluation of biochemical and growth parameters carried out at the dose corresponding to GR50, a significant decrease in the height, fresh and dry weight of the aerial parts, and fresh weight of the roots was found. Substantial increases in the proline concentration and the root/shoot ratio were also observed. There was no significant alteration of the dry weight of the roots and the content of soluble sugars or chlorophylls. Based on these results, it can be concluded that the development of vitroplants of S. rebaudiana var. Eirete is reduced by increasing concentrations of NaCl in the culture medium; however, our results suggest that stevia plants can be cultivated in moderate saline conditions.

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Section: Introductionmentioning

confidence: 99%

Responses of stevia (Stevia rebaudiana Bertoni) to salinity conditions

Piris,

Duré,

Samudio-Oggero

et al. 2023

Acta Agro

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“…Los avances tecnológicos en la micropropagación de sábila han generado una serie de metodologías altamente eficientes en cuanto la cantidad de vitroplantas producidas con la calidad fitosanitaria que conlleva esta tecnología (Albany et al, 2015;Hashem y Kaviani, 2010). No obstante, han surgido nuevos protocolos basados en el uso de medios de cultivo líquidos para algunas de las fases de la micropropagación (Albany et al, 2015;Cardarelli et al, 2014;Vilchez et al, 2007), que permite disminuir los costos de producción, sólo con el hecho de omitir el agente gelificante en los medios de cultivo, lo que favorece la competitividad de las vitroplantas de sábila con respecto al sistema en medios gelificados.…”

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