Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins

Mishra, Kanuj; Stankevych, Mariia; Werner, J.; Grassmann, Simon; Gujrati, Vipul; Huang, Yuanhui; Klemm, Uwe; Buchholz, Veit R.; Ntziachristos, Vasilis; Stiel, André C.

doi:10.1126/sciadv.aaz6293

Cited by 32 publications

(46 citation statements)

References 35 publications

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“…Expression of BphPs also allowed for the segmentation of tumor cells from subcutaneous tumor vasculature in vivo (Märk et al, 2018) and differentiating bacteria injected in tissue (Chee et al, 2018). Expression of selected truncated BphP variants optimized for extinction coefficients, switching kinetics, and reduced photofatigue allowed for the segmentation of as few as 500 cells/ul Jurkat T lymphocytes after subcutaneous injection into mice via classification of a set of features extracted from the photoswitching signal trajectories (Mishra et al, 2020).…”

Section: Semi-and Fully Genetic Targetable Labelsmentioning

confidence: 99%

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Bodea

Westmeyer

2021

Front. Neurosci.

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A prominent goal of neuroscience is to improve our understanding of how brain structure and activity interact to produce perception, emotion, behavior, and cognition. The brain’s network activity is inherently organized in distinct spatiotemporal patterns that span scales from nanometer-sized synapses to meter-long nerve fibers and millisecond intervals between electrical signals to decades of memory storage. There is currently no single imaging method that alone can provide all the relevant information, but intelligent combinations of complementary techniques can be effective. Here, we thus present the latest advances in biomedical and biological engineering on photoacoustic neuroimaging in the context of complementary imaging techniques. A particular focus is placed on recent advances in whole-brain photoacoustic imaging in rodent models and its influential role in bridging the gap between fluorescence microscopy and more non-invasive techniques such as magnetic resonance imaging (MRI). We consider current strategies to address persistent challenges, particularly in developing molecular contrast agents, and conclude with an overview of potential future directions for photoacoustic neuroimaging to provide deeper insights into healthy and pathological brain processes.

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Section: Semi-and Fully Genetic Targetable Labelsmentioning

confidence: 99%

Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience

Bodea

Westmeyer

2021

Front. Neurosci.

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“… 196 - 200 Different photoswitchable probes can also be identified by using a machine-learning-based unmixing method. 197 …”

Section: Technical Advancements In Molecular Patmentioning

confidence: 99%

“…By repeatedly switching the BphPs between the ON and OFF states, the PA signals from the BphPs can be reliably extracted from the non-switching background hemoglobin signals. [196][197][198][199][200] Different photoswitchable probes can also be identified by using a machine-learning-based unmixing method. 197 Activatable probe.…”

Section: Novel Molecular Probes For Deep Patmentioning

confidence: 99%

“…[196][197][198][199][200] Different photoswitchable probes can also be identified by using a machine-learning-based unmixing method. 197 Activatable probe. To improve the detection specificity, a useful strategy in PA molecular imaging is the ability to activate a molecular probe under certain biological and physiological conditions.…”

Section: Novel Molecular Probes For Deep Patmentioning

confidence: 99%

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Sound Out the Deep Colors: Photoacoustic Molecular Imaging at New Depths

Nyayapathi

Kilian

et al. 2020

Mol Imaging

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Photoacoustic tomography (PAT) has become increasingly popular for molecular imaging due to its unique optical absorption contrast, high spatial resolution, deep imaging depth, and high imaging speed. Yet, the strong optical attenuation of biological tissues has traditionally prevented PAT from penetrating more than a few centimeters and limited its application for studying deeply seated targets. A variety of PAT technologies have been developed to extend the imaging depth, including employing deep-penetrating microwaves and X-ray photons as excitation sources, delivering the light to the inside of the organ, reshaping the light wavefront to better focus into scattering medium, as well as improving the sensitivity of ultrasonic transducers. At the same time, novel optical fluence mapping algorithms and image reconstruction methods have been developed to improve the quantitative accuracy of PAT, which is crucial to recover weak molecular signals at larger depths. The development of highly-absorbing near-infrared PA molecular probes has also flourished to provide high sensitivity and specificity in studying cellular processes. This review aims to introduce the recent developments in deep PA molecular imaging, including novel imaging systems, image processing methods and molecular probes, as well as their representative biomedical applications. Existing challenges and future directions are also discussed.

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“…Target analytes that are labeled with different dyes can be clearly distinguished by color differentiation. 1 Multicolor imaging has been widely explored in chemical, biological, and medical fields; this technique can visualize T lymphocytes, bacteria, and tumors in the whole body, 2 constantly monitor drug release from cargos, 3 intensively investigate the spatiotemporal dynamics of signaling cascades, 4 and quantitatively detect the tissue volume. 5 Despite extensive studies, multicolor imaging applications are still limited at the subcutaneous, cellular, or in vitro levels.…”

Section: Introductionmentioning

confidence: 99%