Multiplexed Ultrasensitive Sample‐to‐Answer RT‐LAMP Chip for the Identification of SARS‐CoV‐2 and Influenza Viruses

Abstract

Prompt on‐site diagnosis of SARS‐CoV‐2 with other respiratory infections will have minimized the global impact of the COVID‐19 pandemic through rapid, effective management. However, no such multiplex point‐of‐care (POC) chip has satisfied a suitable sensitivity of gold‐standard nucleic acid amplification tests (NAATs). Here, a rapid multiplexed ultrasensitive sample‐to‐answer loop‐mediated isothermal amplification (MUSAL) chip operated by simple LED‐driven photothermal amplification to detect six targets from single‐swab sampling is presented. First, the MUSAL chip allows ultrafast on‐chip sample preparation with ≈500‐fold preconcentration at a rate of 1.2 mL min−1. Second, the chip enables contamination‐free amplification using autonomous target elution into on‐chip reagents by photothermal activation. Finally, the chip accomplishes multiplexed on‐chip diagnostics of SARS‐CoV‐2 and influenza viruses with a limit of detection (LoD) of 0.5 copies µL−1. The rapid, ultrasensitive, cost‐effective sample‐to‐answer chip with a multiplex capability will allow timely management of various pandemics situations that may be faced shortly.

Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

“…Nucleic acids (NAs) serve as diagnostic biomarkers in accurate and sensitive diagnosis of various diseases including cancer 1 – 3 , genetic disorder 4 , and infectious diseases 5 , 6 . Particularly in infectious diseases, molecular diagnostics, such as polymerase chain reaction (PCR)-based detection, are ideal for early diagnosis due to the swift and substantial accumulation of the viral genome compared to the slower antibody response following symptom onset 7 , 8 .…”

Microfluidic strategies for biomimetic lung chip establishment and SARS-CoV2 study

“…Additionally, 3D bioprinting offers a promising solution for large-scale tissue construction, blood vessel maturation, organ transplantation, and regeneration research [ 80 , 139 , 140 ]. Integrating biosensors, multiplex, and artificial intelligence setups into chips can provide real-time online monitoring of the behavior of SARS-CoV2, including viral infection and transmission, microenvironment changes, and organ function [ 141 , 142 ]. Finally, on-chip multi-omics and high-resolution imaging devices can be used to analyze ultra-early-stage viral infection detection, virus screening, organopathy, and secondary and systemic diseases [ [143] , [144] , [145] ].…”

UbiNAAT: a multiplexed point-of-care nucleic acid diagnostic platform for rapid at-home pathogen detection

“…We demonstrate its ability to detect at least two respiratory pathogens (including influenza A and SARS-CoV-2) from a nasal swab through LAMP amplification and signal captured through a cell phone-based reader. This addresses a current limitation in multiplexed testing, 57–59 as there does not currently exist an inexpensive, quantitative platform that could be administered in home environments. This platform does not require sample preparation by the end user.…”