Multiplexed, multimodal profiling of the intracellular activity, interactions, and druggability of protein variants using LABEL-seq
Jessica J. Simon,
Douglas M. Fowler,
Dustin J. Maly
Abstract:Multiplexed assays of variant effect are powerful tools for assessing the impact of protein sequence variation, but are limited to measuring a single protein property and often rely on indirect readouts of intracellular protein function. Here, we developed LAbeling with Barcodes and Enrichment for biochemicaL analysis by sequencing (LABEL-seq), a platform for the multimodal profiling of thousands of protein variants in cultured human cells. Multimodal measurement of ~20,000 variant effects for ~1,600 BRaf vari… Show more
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