Multiplexed microsatellite markers for seven Metarhizium species

Mayerhofer, Johanna; Lutz, Andy; Widmer, Franco; Rehner, Stephen A.; Leuchtmann, Adrian; Enkerli, J.

doi:10.1016/j.jip.2015.09.006

Cited by 22 publications

(15 citation statements)

References 21 publications

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“…Although molecular tools such as single sequence repeat (SSR) markers have been proven useful to characterize strains of the M. anisopliae species complex, this approach is not adequate for species identification or reconstruction of phylogenetic relationships (Mayerhofer et al, 2015;Castro et al, 2016). CG1123 is currently the only example of M. alvesii, and the results presented here unambiguously support our claim that it is a new species.…”

Section: Discussionmentioning

confidence: 58%

Metarhizium alvesii sp. nov.: A new member of the Metarhizium anisopliae species complex

Lopes

Souza

Rocha

et al. 2018

Journal of Invertebrate Pathology

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A strain within the Metarhizium anisopliae species complex was isolated in 2009 from a soil sample in a banana plantation in the municipality of Quixeré, Northeastern region of Brazil. Previous studies showed that this insect-pathogenic strain does not fit with any current taxon within the M. anisopliae species complex, as determined by both genomic and by mass spectrometric analyses. In the present study, CG1123 (=ARSEF 13308) is shown to be morphologically indistinguishable from most species in this cosmopolitan species complex, whereas multilocus phylogeny confirmed its uniqueness and supports its recognition as a new species, Metarhizium alvesii, in honor of Sérgio Batista Alves, one of the founders of insect pathology in Brazil.

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Section: Discussionmentioning

confidence: 58%

Metarhizium alvesii sp. nov.: A new member of the Metarhizium anisopliae species complex

Lopes

Souza

Rocha

et al. 2018

Journal of Invertebrate Pathology

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“…SSR analysis for genotyping of Metarhizium isolates was performed using SSR markers Ma2049, Ma2054 and Ma2063 (Set I) and Ma195, Ma307 and Ma2287 (Set V) (Mayerhofer et al. 2015 ). DNA extracts were diluted 10 to 100 times, and PCR was performed as described in Mayerhofer et al.…”

Section: Methodsmentioning

confidence: 99%

Assessing effects of the entomopathogenic fungus Metarhizium brunneum on soil microbial communities in Agriotes spp. biological pest control

Mayerhofer

Eckard

Hartmann

et al. 2017

FEMS Microbiology Ecology

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The release of large quantities of microorganisms to soil for purposes such as pest control or plant growth promotion may affect the indigenous soil microbial communities. In our study, we investigated potential effects of Metarhizium brunneum ART2825 on soil fungi and prokaryota in bulk soil using high-throughput sequencing of ribosomal markers. Different formulations of this strain, and combinations of the fungus with garlic as efficacy-enhancing agent, were tested over 4 months in a pot and a field experiment carried out for biological control of Agriotes spp. in potatoes. A biocontrol effect was observed only in the pot experiment, i.e. the application of FCBK resulted in 77% efficacy. Colony counts combined with genotyping and marker sequence abundance confirmed the successful establishment of the applied strain. Only the formulated applied strain caused small shifts in fungal communities in the pot experiment. Treatment effects were in the same range as the effects caused by barley kernels, the carrier of the FCBK formulation and temporal effects. Garlic treatments and time affected prokaryotic communities. In the field experiment, only spatial differences affected fungal and prokaryotic communities. Our findings suggest that M. brunneum may not adversely affect soil microbial communities.

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“…DNA extracts were diluted by 1:10 or 1:100 for PCR analyses. Genotyping of isolates was performed with simple sequence repeat (SSR) marker analysis according to the protocol of Mayerhofer et al (2015). SSR marker set I (Ma2049, Ma2054, Ma2063) and set V (Ma195, Ma327, Ma2287) were amplified in multiplex reactions and amplicon sizes were determined with an ABI 3130XL (Applied Biosystems, USA) using 36 cm capillaries, POP-7 TM and GENESCAN TM 400HD ROX TM as an internal size standard.…”

Section: Monitoring Of the Applied Strainmentioning

confidence: 99%

Response of soil microbial communities to the application of a formulated Metarhizium brunneum biocontrol strain

Mayerhofer

Rauch

Hartmann

et al. 2019

Biocontrol Science and Technology

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Entomopathogenic fungi are used for biological control of insect pests. Metarhizium brunneum Petch (Hypocreales) has potential to control Diabrotica virgifera virgifera LeConte (Chrysomelidae), which is a major pest of maize in North America and has recently invaded Europe. The inundative application of an entomopathogenic fungal strain in biological control results in high densities of fungal propagules in the soil which can potentially affect soil microbial communities and their multiple functions in soil. The objective of the present study was to assess potential effects of M. brunneum on soil fungal and prokaryotic communities in a pot experiment over a time course of 4 months using high-throughput sequencing (HTS) of ribosomal markers. The application of M. brunneum formulated as fungus colonised barley kernels (FCBK) led to a significant increase of the applied strain in soil, as assessed by cultivation-dependent (plating on selective medium followed by genotyping of Metarhizium isolates) and cultivation-independent (HTS of ribosomal markers) approaches. Data revealed that soil fungal and prokaryotic community structures did not change after the application of M. brunneum. Temporal changes of the fungal and prokaryotic communities were observed and the prokaryotic communities showed minor changes to barley kernels (BK), the matrix of the formulation. Results of this study are in accordance with other investigations lacking any evidence for adverse effects on microbial communities caused by applied entomopathogenic fungi.

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Multiplexed microsatellite markers for seven Metarhizium species

Cited by 22 publications

References 21 publications

Metarhizium alvesii sp. nov.: A new member of the Metarhizium anisopliae species complex

Metarhizium alvesii sp. nov.: A new member of the Metarhizium anisopliae species complex

Assessing effects of the entomopathogenic fungus Metarhizium brunneum on soil microbial communities in Agriotes spp. biological pest control

Response of soil microbial communities to the application of a formulated Metarhizium brunneum biocontrol strain

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