Multiplexed inkjet functionalization of silicon photonic biosensors

Kirk, James T.; Fridley, Gina E.; Chamberlain, Jeffrey W.; Christensen, Elijah; Hochberg, Michael; Ratner, Daniel M.

doi:10.1039/c0lc00313a

Cited by 67 publications

(63 citation statements)

References 21 publications

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“…For filling these small laboratories with minute amounts of fluid dosing devices / inkjet printheads are necessary. The advantage of being able to eject many different fluids, such as proteins, glycoproteins, or neoglycoconjugates, makes inkjet printheads unavoidable for filling lab-on-a-chip systems [6,7].…”

Section: Introductionmentioning

confidence: 99%

Piezo inkjet drop-on-demand experimentation platform manufactured with rapid prototyping techniques enabling future technologies

Kagerer

Eiler

Ottnad

et al. 2012

2012 IEEE International Conference on Robotics and Biomimetics (ROBIO)

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A novel experimentation platform, which is based on a piezoelectrically driven inkjet printhead and on a support plate, is presented. A huge number of fluids has to be ejected due to the large variety of possible applications for inkjet printheads. Each fluid with its special characteristics usually requires a redesign of the printhead to be able to be ejected.This inkjet printhead is manufactured in a batch process with rapid prototyping techniques in order to be able to be adapted to new boundary conditions in a time saving manner. The manufacturing time only amounts less than 30 minutes. The inkjet printhead is inserted into a support plate. Here, it is electrically as well as fluidically connected without any soldering or gluing processes. Heating elements, temperature as well as pressure sensors, and a fluid reservoir are integrated. The reproducibility of experiments is thereby given. Furthermore, printing fluids with solid-liquid phase transition is possible. The inkjet printhead can be changed within only one minute.

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Section: Introductionmentioning

confidence: 99%

Piezo inkjet drop-on-demand experimentation platform manufactured with rapid prototyping techniques enabling future technologies

Kagerer

Eiler

Ottnad

et al. 2012

2012 IEEE International Conference on Robotics and Biomimetics (ROBIO)

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“…Inkjet printing confers more advantages: in the field of nanotechnologies, it has been exploited to produce a formulation capable of controlling the release of a drug [20], to better functionalize biosensors [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Nanoprobe NAPPA Arrays for the Nanoconductimetric Analysis of Ultra-Low-Volume Protein Samples Using Piezoelectric Liquid Dispensing Technology

Pechkova¹,

Wiktor²,

Bragazzi³

et al. 2015

NanoWorld J

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In the last years, the evolution and the advances of the nanobiotechnologies applied to the systematic study of proteins, namely proteomics, both structural and functional, and specifically the development of more sophisticated and largescale protein arrays, have enabled scientists to investigate protein interactions and functions with an unforeseeable precision and wealth of details. Here, we present a further advancement of our previously introduced and described Nucleic Acid Programmable Protein Arrays (NAPPA)-based nanoconductometric sensor. We coupled Quartz Crystal Microbalance with Dissipation factor Monitoring (QCM_D) with piezoelectric inkjet printing technology (namely, the newly developed ActivePipette), which enables to significantly reduce the volume of probe required for genes/proteins arrays. We performed a negative control (with master mix, or MM) and a positive control (MM_p53 plus MDM2). We performed this experiment both in static and in flow, computing the apparent dissociation constant of p53-MDM2 complex (130 nM, in excellent agreement with the published literature). We compared the results obtained with the ActivePipette printing and dispensing technology vs. pin spotting. Without the ActivePipette, after MDM2 addition the shift in frequency (Δf ) was 7575 Hz and the corresponding adsorbed mass was 32.9 µg. With the ActivePipette technology, after MDM2 addition Δf was 7740 Hz and the corresponding adsorbed mass was 33.6 µg. With this experiment, we confirmed the sensing potential of our device, being able to discriminate each gene and protein as well as their interactions, showing for each one of them a unique conductance curve. Moreover, we obtained a better yield with the ActivePipette technology.

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“…high-refractive-index silicon nanophotonic sensor platforms in various extensions have been developed, with the advantages of high sensitivity and measurement in the presence of the sample without any rinsing (9). Waveguide sensors constructed in silicon nanophotonic platforms can have an extremely small footprint, be integrated into compact arrays, and be readily combined with fluidic components, showing promising features for potential lab-ona-chip applications (3).…”

Section: Introductionmentioning

confidence: 99%