Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor
Yaokang Wu,
Yang Li,
Yanfeng Liu
et al.
Abstract:Mutagenesis driving genetic diversity is vital for understanding and engineering biological systems. However, the lack of effective methods to generate in-situ mutagenesis in multiple genomic loci combinatorially limits the study of complex biological functions. Here, we design and construct MultiduBE, a dCas12a-based multiplexed dual-function base editor, in an all-in-one plasmid for performing combinatorial in-situ mutagenesis. Two synthetic effectors, duBE-1a and duBE-2b, are created by amalgamating the fun… Show more
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