Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants

Kellar, Kathryn L.; Kalwar, Rizwan R.; Dubois, Kimberly A.; Crouse, Dennis T.; Chafin, William D.; Kane, Beth-Ellen

doi:10.1002/1097-0320(20010901)45:1<27::aid-cyto1141>3.0.co;2-i

Cited by 183 publications

(151 citation statements)

References 13 publications

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“…Concentrations of IL-4 and IL-5 in whole lung lavage fluid were determined using commercial multiplexed fluorescent bead-based immunoassays (Upstate Biotechnology, Lake Placid, NY), similar to those described previously (23). Briefly, standard concentrations of the respective cytokines (50 l) were placed in duplicate into the wells of a 96-well filtration plate.…”

Section: Methodsmentioning

confidence: 99%

Allergen-induced airway disease is mouse strain dependent

Whitehead

Walker

Berman

et al. 2003

American Journal of Physiology-Lung Cellular and Molecular Phys

168

119

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We investigated the development of airway hyperreactivity (AHR) and inflammation in the lungs of nine genetically diverse inbred strains of mice [129/SvIm, A/J, BALB/cJ, BTBR+(T)/tf/tf, CAST/Ei, C3H/HeJ, C57BL/6J, DBA/2J, and FVB/NJ] after sensitization and challenge with ovalbumin (OVA). At 24, 48, and 72 h post-OVA exposure, the severity of AHR and eosinophilic inflammation of the mouse strains ranged from relatively unresponsive to responsive. The severity of the airway eosinophilia of some strains did not clearly correlate with the development of AHR. The temporal presence of T helper type 2 cytokines in lung lavage fluid also varied markedly among the strains. The levels of IL-4 and IL-13 were generally increased in the strains with the highest airway eosinophilia at 24 and 72 h postexposure, respectively; the levels of IL-5 were significantly increased in most of the strains with airway inflammation over the 72-h time period. The differences of physiological and biological responses among the inbred mouse strains after OVA sensitization and challenge support the hypothesis that genetic factors contribute, in part, to the development of allergen-induced airway disease.

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Section: Methodsmentioning

confidence: 99%

Allergen-induced airway disease is mouse strain dependent

Whitehead

Walker

Berman

et al. 2003

American Journal of Physiology-Lung Cellular and Molecular Phys

168

119

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“…The MBA is at least as sensitive as ELISA, and as shown in this study, it was sufficiently sensitive to provide valuable sero-epidemiological data, including the pronounced agedependent downward shift in responses to the Giardia VSP antigens. [36][37][38][39][40][41] …”

Section: Estimated Mean Immunoglobulin G (Igg) Responses Tomentioning

confidence: 99%

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Moss

Priest

Hamlin³

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for Giardia intestinalis and Entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from G. intestinalis (variant-specific surface protein [VSP1-VSP5]), E. histolytica (lectin adhesion molecule [LecA]), and Cryptosporidium parvum (17-and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (P 0.002) in children 4.5 years than in children 4.5 years. The IgG responses to Cryptosporidium tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (P 0.037 and P 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.

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“…Since this technique simultaneously induces fluid motion to rapidly and continuously concentrate a sample, REP can be used to improve the efficiency of lab-on-a-chip sensors and processes, including bead-based assays [7]. In addition, REP colloidal accumulation is frequency dependent, enabling sorting and characterization of species based on their ac electrokinetic behavior.…”

Section: Introductionmentioning

confidence: 99%

Optically induced electrokinetic concentration and sorting of colloids

Williams

Kumar

Green

et al. 2009

J. Micromech. Microeng.

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We demonstrate an optically induced ac electrokinetic technique that rapidly and continuously accumulates colloids on a parallel-plate electrode surface resulting in a crystalline-like aggregation. Electrothermal hydrodynamics produce a microfluidic vortex that carries suspended particles toward its center where they are trapped by local ac electrokinetic hydrodynamic forces. We characterize the rate of particle aggregation as a function of the applied ac voltage, ac frequency and illumination intensity. Hundreds of polystyrene particles (1.0 μm) suspended in a low conductivity solution (2.4 mS m −1 ) were captured at a range of voltages (5-20 V pp ) and frequencies (20-150 kHz) with an optical power of approximately 20 mW. This technique was not restricted to near infrared (1064 nm) illumination and was also demonstrated at 532 nm. The sorting capability of this technique was demonstrated with a solution containing 0.5 μm, 1.0 μm and 2.0 μm polystyrene particles. This dynamic optically induced technique rapidly concentrates, sorts and translates colloidal aggregates with a simple parallel-plate electrode configuration and can be used for a variety of lab-on-a-chip applications.

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Multiplexed fluorescent bead-based immunoassays for quantitation of human cytokines in serum and culture supernatants

Cited by 183 publications

References 13 publications

Allergen-induced airway disease is mouse strain dependent

Allergen-induced airway disease is mouse strain dependent

Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

Optically induced electrokinetic concentration and sorting of colloids

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