Multiplexed electrochemical assays for clinical applications

Sharafeldin, Mohamed; Rusling, James F.

doi:10.1016/j.coelec.2023.101256

Cited by 10 publications

(21 citation statements)

References 67 publications

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“…In addition, the same miRNA can be determined simultaneously in different samples. These potential abilities entail important advantages in terms of shorter overall analysis times, smaller sample volume, reduced cost, and more accurate diagnostics . In this context, the developed bioplatform may be envisaged as an attractive tool to shed light on the miRNA methylome and to identify new molecular signatures of methylated miRNAs with diagnostic, prognostic, and therapeutic value.…”

Section: Resultsmentioning

confidence: 99%

Bringing to Light the Importance of the miRNA Methylome in Colorectal Cancer Prognosis Through Electrochemical Bioplatforms

Povedano,

Ruiz-Valdepeñas Montiel,

Sebuyoya

et al. 2024

Anal. Chem.

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This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic biotinylated DNA probe, the capture of the formed DNA/miRNA heterohybrids on the surface of magnetic microcarriers, and their recognition with an antibody selective to these heterohybrids or to the N 6 -methyladenosine (m6A) epimark. The determination of the total or methylated target miRNA was accomplished by labeling such secondary antibodies with the horseradish peroxidase (HRP) enzyme. In both cases, amperometric transduction was performed on the surface of disposable electrodes after capturing the resulting HRP-tagged magnetic bioconjugates. Because of their increasing relevance in colorectal cancer (CRC) diagnosis and prognosis, miRNA let-7a and m6A methylation were selected. The proposed electrochemical bioplatforms showed attractive analytical and operational characteristics for the determination of the total and m6A-methylated target miRNA in less than 75 min. These bioplatforms, innovative in design and application, were applied to the analysis of total RNA samples extracted from cultured cancer cells with different metastatic profiles and from paired healthy and tumor tissues of patients diagnosed with CRC at different stages. The obtained results demonstrated, for the first time using electrochemical platforms, the potential of interrogating the target miRNA methylation level to discriminate the metastatic capacities of cancer cells and to identify tumor tissues and, in a pioneering way, the potential of the m6A methylation in miRNA let-7a to serve as a prognostic biomarker for CRC.

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Section: Resultsmentioning

confidence: 99%

Bringing to Light the Importance of the miRNA Methylome in Colorectal Cancer Prognosis Through Electrochemical Bioplatforms

Povedano,

Ruiz-Valdepeñas Montiel,

Sebuyoya

et al. 2024

Anal. Chem.

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“…[94] Despite recent developments in the utilization of peptides and MIPs as biorecognition elements in the construction of electrochemical biosensors, a notable gap exists in research focused on the construction of multiplex platforms using electrode arrays. Also, aptamers and antibodies are used to construct molecular pendulum [95] and molecular switch-based biosensor [96] platforms that have not been used for multiplexed detection, [15] however, they may be used in the future. Therefore, it is advisable to conduct thorough investigations into these alternative materials before embarking on the biosensor construction process.…”

Section: Discussionmentioning

confidence: 99%

“…Strategies for multiplexing with electrochemical biosensing arrays for cancer biomarkers and clinical applications have been previously reviewed. [14,15] In contemporary electrochemical biosensor applications, multiplex detection can be achieved by two prominent methods. The first involves the use of a single electrode designed to incorporate a diverse array of bioreceptors, while the second uses an electrode array, with each individual electrode is functionalized to accommodate a distinct bioreceptor.…”

Section: Introductionmentioning

confidence: 99%

“…A recent and exhaustive review conducted by Sharafeldin and Rusling explored these multifaceted multiplexing strategies. [15] The aim of this concept article is to provide comprehensive guidance for the beginners in this field on developing multiplex electrochemical biosensing platforms commencing from the fabrication of electrode arrays and extending to the sophisticated strategies involved in immobilizing diverse bioreceptor types, which are prerequisites for multiple analyte detection.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Biosensor Arrays for Multiple Analyte Detection

Sen,

Lazenby

2023

Analysis & Sensing

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The simultaneous detection of multiple analytes using electrochemical biosensor arrays has demonstrated significant potential in various fields, including medical diagnostics. These platforms are designed to be low‐cost, easy to use, and offer fast detection of a range of molecules. This concept article discusses the components necessary to achieve multiplexing with electrochemical biosensor arrays. The various methods used to fabricate electrode arrays of different types are discussed. Furthermore, the methods for the selective immobilization of multiple different bioreceptors onto individual electrodes within the array, a crucial step essential for conferring specificity to the analytical processes, are presented with relevant examples. We have focused on enzymes, antibodies and aptamers as examples of bioreceptors that have been deployed in various multianalyte electrochemical detection platforms. Finally, we discuss the key challenges associated with their application for the analysis of real samples and provide a future outlook on possible strategies that can be used to overcome these challenges.

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“…The ability to achieve multiplexed capture in a single chamber that is not functionalized specifically for the targets in question also increases the flexibility of the platform to switch between different assays compared to other multiplexed platforms that rely on compartmentalization with specific binding agents for each target functionalized beforehand to each compartment. 20,21 While several assays exist that achieve multiplexed detection of biomarkers on a single electrode by the use of electrochemical tags, [40][41][42] these have still relied on specific functionalization of the device to achieve specific detection. Additionally, while affinity-based electrochemical biosensors where the electrical signal comes from targets binding directly to electrode sensors can suffer issues from biofouling.…”

Section: On-chip Multiplexed Virus Detection In Plasma Samplesmentioning

confidence: 99%