Abstract:A multiplexed DNA detection strategy with fast hybridization kinetics based on positional encoding/decoding with self-assembled DNA nanostructures has been developed.
“…154 DNA self-assembly has also been used to generate barcodes with regards to the shape of its 3D nanostructure. 20 The different shapes of the self-assembled DNA can be well observed and distinguished under TEM imaging. However, the size and shape based barcode technique cannot generate many distinct and unique barcodes as compared to other encoding techniques.…”
Section: Morphological Encodingmentioning
confidence: 97%
“…With the availability of a plethora of nanobarcodes, they have been employed for many applications such as detection of biomolecules (nucleic acid and proteins), imaging, security, drug delivery, theranostics, etc. 7,11,[14][15][16][17][18][19][20][21] Decoding of signals from these nano-barcodes depends on their encoding technique. For instance, nano-barcodes with fluorescent encoding elements requires fluorescence microscopy or spectra measurement for detection, whereas nano-barcodes of different light reflectivity pattern can be read out by reflectance optical microscopy.…”
Section: Thoriq Salafimentioning
confidence: 99%
“…17a-c. 20,225,226 DNA based self-assembly has been established for more than three decades, and the generation of the DNA strands is fast and relatively cheap. However, the DNA based self-assembly method is not stable at high temperature or extreme pH as the selfassembled DNA can be denatured.…”
Encoded nano-structures/particles have been used for barcoding and are in great demand for the simultaneous analysis of multiple targets. Due to their nanoscale dimension(s), nano-barcodes have been implemented favourably for bioimaging, in addition to their security and multiplex bioassay application. In designing nano-barcodes for a specific application, encoding techniques, synthesis strategies, and decoding techniques need to be considered. The encoding techniques to generate unique multiple codes for nano-barcodes are based on certain encoding elements including optical (fluorescent and non-fluorescent), graphical, magnetic, and phase change properties of nanoparticles or their different shapes and sizes. These encoding elements can generally be embedded inside, decorated on the surface of nanostructures or self-assembled to prepare the nano-barcodes. The decoding techniques for each encoding technique are different and need to be suitable for the desired applications. This review will provide a thorough discussion on designing nano-barcodes, focusing on the encoding techniques, synthesis methods, and decoding for applications including bio-detection, imaging, and anti-counterfeiting. Additionally, associated challenges in the field and potential solutions will also be discussed. We believe that a comprehensive understanding on this topic could significantly contribute towards the advancement of nano-barcodes for a broad spectrum of applications.
“…154 DNA self-assembly has also been used to generate barcodes with regards to the shape of its 3D nanostructure. 20 The different shapes of the self-assembled DNA can be well observed and distinguished under TEM imaging. However, the size and shape based barcode technique cannot generate many distinct and unique barcodes as compared to other encoding techniques.…”
Section: Morphological Encodingmentioning
confidence: 97%
“…With the availability of a plethora of nanobarcodes, they have been employed for many applications such as detection of biomolecules (nucleic acid and proteins), imaging, security, drug delivery, theranostics, etc. 7,11,[14][15][16][17][18][19][20][21] Decoding of signals from these nano-barcodes depends on their encoding technique. For instance, nano-barcodes with fluorescent encoding elements requires fluorescence microscopy or spectra measurement for detection, whereas nano-barcodes of different light reflectivity pattern can be read out by reflectance optical microscopy.…”
Section: Thoriq Salafimentioning
confidence: 99%
“…17a-c. 20,225,226 DNA based self-assembly has been established for more than three decades, and the generation of the DNA strands is fast and relatively cheap. However, the DNA based self-assembly method is not stable at high temperature or extreme pH as the selfassembled DNA can be denatured.…”
Encoded nano-structures/particles have been used for barcoding and are in great demand for the simultaneous analysis of multiple targets. Due to their nanoscale dimension(s), nano-barcodes have been implemented favourably for bioimaging, in addition to their security and multiplex bioassay application. In designing nano-barcodes for a specific application, encoding techniques, synthesis strategies, and decoding techniques need to be considered. The encoding techniques to generate unique multiple codes for nano-barcodes are based on certain encoding elements including optical (fluorescent and non-fluorescent), graphical, magnetic, and phase change properties of nanoparticles or their different shapes and sizes. These encoding elements can generally be embedded inside, decorated on the surface of nanostructures or self-assembled to prepare the nano-barcodes. The decoding techniques for each encoding technique are different and need to be suitable for the desired applications. This review will provide a thorough discussion on designing nano-barcodes, focusing on the encoding techniques, synthesis methods, and decoding for applications including bio-detection, imaging, and anti-counterfeiting. Additionally, associated challenges in the field and potential solutions will also be discussed. We believe that a comprehensive understanding on this topic could significantly contribute towards the advancement of nano-barcodes for a broad spectrum of applications.
“…20 However, addressing the fabrication of higher order DNA nanomaterials non-covalently with the same precision as that of DNA origami without compromising the intrinsic dynamic and functional properties remains as a challenging task. 21 This could decrease the cost of synthesis and tedious periodic assembly of long multiple DNA components. 22 On the other hand, DNA has been used as a scaffold or template in the bottom-up assembly of metal ions, [23][24][25] small molecules, 26 oligomers, 27,28 polymers, 29,30 and nanoparticles, 31,32 into various nanostructures.…”
Self-assembly of F-An nanoclusters with 3WJ-DNA and 3WJ-OH offers nanosheets and entangled 2D-nanonetworks, respectively. 3WJ-OH/F-An in the presence of AgNCs shows enhanced fluorescence (∼40%) due to its stabilization in the 2D-nanonetworks.
“…1–3 DNA nanotechnology uses DNA strands as biological engineering materials to create static structures that perform mechanical operations such as DNA machines, including “DNA tweezers”, “DNA walkers”, etc. 4–6 Synthetic DNA machines have been developed to mimic important biological processes in vitro .…”
We report a DNA walking biosensor that can realize the detection of let-7a with a detection limit of 58 fM and high selectivity for resolving one nucleotide variation.
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