Multiplexed detection of tumor markers with multicolor quantum dots based on fluorescence polarization immunoassay

Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip

“…Various approaches have been proposed to perform simultaneous detection of multiple tumor markers in the past few years. For example, electrochemical immunoassay (Wilson, 2007;Xu et al, 2014), label-free methods (Lin et al, 2011;Kong et al, 2013), dielectrophoresi assays (Lee et al, 2010;Ramón-Azcón et al, 2011), chemiluminescence alalysis (Zong et al, 2012;Guo et al, 2013), biobarcoded Scanometric assay (Stoeva et al, 2006), spectrometry (Chon et al, 2011;Giesen et al, 2011), fluorescence detection (Tian et al, 2012;Akinfieva et al, 2013), photonic suspension array (Zhao et al, 2009). But all these techniques are not suitable for point-of-care testing because they require large and sophisticated instruments, complex operations, long analysis time and highly skilled personnel to manipulate.…”

A droplet-based fluorescence polarization immunoassay (dFPIA) platform for rapid and quantitative analysis of biomarkers

“…Heterogeneous assays typically require analyte immobilization, multiple washing steps, and the physical separation of target bound complexes from non-target species prior to detection (Tachi et al, 2009;Tian et al, 2012). Traditionally, lateral flow assays (LFAs) and enzyme-linked immunosorbent assays (ELISAs) have been most commonly used to detect biomarkers in the human sera, urine, tears and food products (Kiening et al, 2005;Van Coillie et al, 2004;Wen et al, 2005aWen et al, , 2005b.…”

“…To overcome these drawbacks fluorescence polarization immunoassays (FPIAs) have been increasingly used for biomarker detection (Tachi et al, 2009;Tian et al, 2012;Wang et al, 2011;Xu et al, 2011). FPIAs are homogeneous immunoassays, rapid and simple to implement and do not require antibody immobilization or washing steps.…”

Simple and effective label-free electrochemical immunoassay for carbohydrate antigen 19-9 based on polythionine-Au composites as enhanced sensing signals for detecting different clinical samples

“…Traditional immunoassay methods for tumor markers include fluorescence, 11,12 spectroscopy, 13,14 chemiluminescence, 15,16 radioimmunoassay, 17,18 electrophoresis, 19 polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA). [20][21][22] Current methods can obtain accurate and reliable detection results but usually require expensive instruments and complex operating procedures.…”