Multiplexed detection of nucleic acids using ¹⁹F NMR chemical shift changes based on DNA photo-cross-linking of 3-vinylcarbazole derivatives

Abstract

The detection methodology for nucleic acids is a useful tool for the analysis of biological systems and diagnosis of diseases. We demonstrated the feasibility of the detection of any nucleic acids based on large chemical shifts via ultrafast DNA photo-cross-linking and the effects of substitution by 3-vinylcarbazole derivatives. These chemical shifts enable the sequence-specific detection of any strand using hybridization chain reaction.

Light responsive nucleic acid for biomedical application

“…This method can control the aggregation of nucleic acid motifs, such as controlling the switching of DNA motifs between gel and sol. [83] Besides, Nakamura and coworkers [96] developed a nucleic acid sequence-specific detection method. As shown in Figure 8C, the specific nucleic acid sequence detection system consists of two DNA hairpin probes (probe A and probe B).…”

“…Hybridization/ dehybridization [91] Attachment to nucleobases Hybridization [94] Substituted nucleobases Photocrosslinking [96] Backbone insertion Strand flip [100] Substituted nucleotides Hybridization/ dehybridization [101] End-capping elF4E affinity/non-affinity [102] For example, the photo-induced structural changes of light responsive nucleic acids make them good drug carriers and biosensors. As a natural genetic material, light responsive nucleic acid is a homolog of the genetic material in the cell and is easier to interact with it.…”

“…Reproduced with permission. [ 96 ] Copyright 2018, Royal Society of Chemistry. (D) Schematic diagram of reversible light control of siRNAzos.…”

“…Besides, Nakamura and coworkers [ 96 ] developed a nucleic acid sequence‐specific detection method. As shown in Figure 8C, the specific nucleic acid sequence detection system consists of two DNA hairpin probes (probe A and probe B).…”

Crosslinker-modified nucleic acid probes for improved target identification and biomarker detection

“…Applications of cyanovinyl-based crosslinkers are not limited to the examples given above. Indeed, these fascinating constructs have paved their way in applications such as 19 F-NMR based detection of nucleic acid sequences, 63 in DNA-peptide photo-crosslinking, 64 in DNA assemblies 65 and in antisense applications 66,67 to name a few.…”