Multiplexed Detection and Label‐Free Quantitation of MicroRNAs Using Arrays of Silicon Photonic Microring Resonators

Abstract

Microrings for microRNAs! A label‐free method has been developed for the sensitive detection of microRNAs utilizing arrays of silicon photonic microring resonators. This simple and modularly multiplexable method for the direct profiling of microRNA within 10 minutes meets a number of challenges faced by current methodologies in this area.

miRNA Therapeutics: Delivery and Biological Activity of Peptide Nucleic Acids Targeting miRNAs

“…This is clearly depicted in Figure 5, which sustains the concept that treatment with PNAs against miRNAs leads to selective effects. Furthermore, a significant challenge for all nucleic acid analyses, that is particularly important for alteration of miRNAs biological functions, is the ability to distinguish single-base differences in sequence [100]. In this case, PNAs are expected to be important for discrimination between miRNA isoforms, since these reagents enable the identification of fully-matched versus mismatched miRNA sequences [101].…”

Silicon microring resonators

“…Single-parameter measurements only provide limited detail and quantification of multiple biomolecular signatures can more fully illuminate complex biological functions [160,169] An array of silicon-on-insulator ring resonator biosensors has already been applied to simultaneously detect multiple proteins [166,167,172] and oligonucleotides [171,173]. 67 To conclude, silicon-on-insulator ring resonators have in recent years been proven to be excellent transducers for label-free biosensing, comparing favorably with other labelfree sensing techniques.…”

Ultrasensitive detection of a protein by optical trapping in a photonic‐plasmonic microcavity

“…The WGM biosensing approach has been shown to be highly sensitive down to the single virus or nanobeads level ($100 nm) [22,23,25,26] in purified buffer solutions. In complex samples such as blood plasma, recognition elements such as antibodies or DNA oligonucleotides immobilized at the WGM microsphere surface are needed for specific detection tasks [27,28].…”