Multiplexed biosensor for point-of-care COVID-19 monitoring: CRISPR-powered unamplified RNA diagnostics and protein-based therapeutic drug management

Johnston, Midori; Ates, H. Ceren; Glatz, Regina; Mohsenin, Hasti; Schmachtenberg, Rosanne; Göppert, Nathalie; Huzly, Daniela; Urban, G.; Weber, Wilfried; Dincer, Can

doi:10.1016/j.mattod.2022.11.001

Cited by 23 publications

(17 citation statements)

References 72 publications

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“…Multiplexed electrochemical microfluidic biosensors have been developed for on-site measurement of multiple analytes from one sample and/or of the same analyte from different samples simultaneously, thus enhancing the accuracy of the diagnosis of diseases and their therapy success. ,, …”

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

“…In addition, electrochemical affinity bioplatforms involving aptamer switches and penicillin binding proteins (PBPs, bacterial proteins that bind only the active form of penicillin and other antibiotics of the β-lactam class) have demonstrated to meet the challenging demands of therapeutic drug monitoring (TDM). Remarkable examples are the bioplatforms implemented on gold wires or needles for in vivo continuous, real-time monitoring of drugs in live rats, and the multiplexed microfluidic bioplatform displayed in Figure for the simultaneous determination of viral load (viral E RNA and RdRP genes using assays enhanced by CRISPR/Cas, gene editing technology discussed in more detail in the following subsection) and ß-lactam antibiotic in nasal swabs and serum from COVID-19-infected patients, which allowed near real-time assessment of the efficacy of therapy for the treated infection …”

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

“…Multiplexed microfluidic bioplatform for the simultaneous determination of viral load and ß-lactam antibiotic in nasal swabs and serum samples from COVID-19-infected patients. Reprinted with permission . Copyright 2022, Elsevier.…”

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

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Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

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Electrochemical affinity biosensors are evolving at breakneck speed, strengthening and colonizing more and more niches and drawing unimaginable roadmaps that increasingly make them protagonists of our daily lives. They achieve this by combining their intrinsic attributes with those acquired by leveraging the significant advances that occurred in (nano)materials technology, bio(nano)materials and nature-inspired receptors, gene editing and amplification technologies, and signal detection and processing techniques. The aim of this Perspective is to provide, with the support of recent representative and illustrative literature, an updated and critical view of the repertoire of opportunities, innovations, and applications offered by electrochemical affinity biosensors fueled by the key alliances indicated. In addition, the imminent challenges that these biodevices must face and the new directions in which they are envisioned as key players are discussed.

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Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

Section: Key Alliances To Cover Important Routesmentioning

confidence: 99%

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Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

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“…This method is able to identify individuals infected with variants of SARS-CoV-2, and can be done without cross-contamination. 43 A label-free biosensing device for TDM has been developed to detect infliximab therapeutic antibodies using plastic optical fiber (POF). The device uses the SPR sensing technique to detect specific binding between anti-IFX antibody immobilized on the POF sensing region and IFX diluted in buffer and human serum.…”

Section: Pencil Graphite Electrode Basedmentioning

confidence: 99%

Biosensors for therapeutic drug monitoring: a review

et al. 2023

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Therapeutic drug monitoring (TDM) is a crucial and essential step for patient care when an accurate medication dosage is necessary. High-performance liquid chromatography (HPLC) and immunoassays are commonly used methods for TDM, but they are expensive and incapable of real-time monitoring. Biosensor technology is believed to have the potential to perform TDM effectively. Biosensors are flexible and can be tailored to individual patient needs. This article reviews the development of biosensors for TDM, including the types of biosensors that have been fabricated and the drugs they have successfully monitored. Biosensor technology is expected to have a bright future, particularly for real-time monitoring and integration with internet of things (IoT) systems.

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“…Generally, the SARS-CoV-2 variants differed in several nucleotides or even single-nucleotide mutation, and the thermodynamic stability difference between single-nucleotide mutated sequences and sequences without mutations is particularly negligible. − (ii) Rapid readout of detection results. Currently developed methods for the identification of SARS-CoV-2 variants require a time-consuming nucleic acid amplification process, and the turnaround time from sample-to-result costs at least 30 min, making it inadaptable for rapid and scalable variants screening. − (iii) Capable of detecting multiplexed RNA mutations simultaneously. SARS-CoV-2 VOCs involve a huge number of sublineages and sets of corresponding RNA mutations. ,, The detection of multiple mutated RNAs simultaneously is expected to improve the identification efficiency of RNA mutations and the ability in differentiating SARS-CoV-2 variants. − Therefore, the development of an efficient nucleic acid detection assay with the capability to accurately identify viral genome SNPs is highly demanded to achieve rapid SARS-CoV-2 variants screening.…”

mentioning

confidence: 99%

Electrical Detection Assay Based on Programmable Nucleic Acid Probe for Efficient Single-Nucleotide Polymorphism Identification

Zhang,

Chen,

Chen

et al. 2023

ACS Sens.

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The large-scale pandemic and fast evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have triggered an urgent need for an efficient and sensitive on-site nucleic acid testing method with single-nucleotide polymorphism (SNP) identification capability. Here, we report a multiplexed electrical detection assay based on a paperclip-shaped nucleic acid probe (PNprobe) functionalized field-effect transistor (FET) biosensor for highly sensitive and specific detection and discrimination of SARS-CoV-2 variants. The three-stem structure of the PNprobe significantly amplifies the thermodynamic stability difference between variant RNAs that differ in a single-nucleotide mutation. With the assistance of combinatorial FET detection channels, the assay realizes simultaneously the detection and identification of key mutations of seven SARS-CoV-2 variants, including nucleotide substitutions and deletions at single-nucleotide resolution within 15 min. For 70 simulated throat swab samples, the multiplexed electrical detection assay shows an identification accuracy of 97.1% for the discrimination of SARS-CoV-2 variants. Our designed multiplexed electrical detection assay with SNP identification capability provides an efficient tool to achieve scalable pandemic screening.

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Multiplexed biosensor for point-of-care COVID-19 monitoring: CRISPR-powered unamplified RNA diagnostics and protein-based therapeutic drug management

Cited by 23 publications

References 72 publications

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Biosensors for therapeutic drug monitoring: a review

Electrical Detection Assay Based on Programmable Nucleic Acid Probe for Efficient Single-Nucleotide Polymorphism Identification

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