Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Weir, Kurt; Leavey, Patrick J.; Santiago, Clayton; Blackshaw, Seth

doi:10.3791/62239

Cited by 3 publications

(1 citation statement)

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“…Still, matching studies that investigate retinal chromatin accessibility dynamics at the single cell resolution remain limited ( Xie et al, 2020 ). With recent technical advances that enable the simultaneous profiling of transcriptome and epigenome from the same cells, it is almost certain that work is underway to accurately outlining the epigenome dynamics in relation to gene expression in retinal cell populations ( Kashima et al, 2020 ; Weir et al, 2021 ).…”

Section: Discovery Of Retinal Enhancersmentioning

confidence: 99%

Building a Mammalian Retina: An Eye on Chromatin Structure

Daghsni

Aldiri

2021

Front. Genet.

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Regulation of gene expression by chromatin structure has been under intensive investigation, establishing nuclear organization and genome architecture as a potent and effective means of regulating developmental processes. The substantial growth in our knowledge of the molecular mechanisms underlying retinogenesis has been powered by several genome-wide based tools that mapped chromatin organization at multiple cellular and biochemical levels. Studies profiling the retinal epigenome and transcriptome have allowed the systematic annotation of putative cis-regulatory elements associated with transcriptional programs that drive retinal neural differentiation, laying the groundwork to understand spatiotemporal retinal gene regulation at a mechanistic level. In this review, we outline recent advances in our understanding of the chromatin architecture in the mammalian retina during development and disease. We focus on the emerging roles of non-coding regulatory elements in controlling retinal cell-type specific transcriptional programs, and discuss potential implications in untangling the etiology of eye-related disorders.

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Section: Discovery Of Retinal Enhancersmentioning

confidence: 99%