Multiplex <scp>PCR</scp> scheme for variant plasmid mediated class C β‐lactamase typing

Chuong, Le Van; Prachayasittikul, Virapong; Isarankura‐Na‐Ayudhya, Chartchalerm; Lawung, Ratana

doi:10.1002/jcla.22298

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…The detection of ESBL genes (bla CTX-M , bla SHV , bla OXA , and bla TEM genes) was carried out by PCR and DNA sequencing under previously described conditions (55). In addition, strains resistant to amoxicillin-clavulanic acid were tested for cefoxitin susceptibility by Etest in order to confirm the presence of cephamycinases, which were also detected by PCR and DNA sequencing using specific primers, as described previously (56).…”

Section: Methodsmentioning

confidence: 99%

Emergence of Resistance to Quinolones and β-Lactam Antibiotics in Enteroaggregative and Enterotoxigenic Escherichia coli Causing Traveler’s Diarrhea

Guiral

Quiles

Muñoz

et al. 2019

Antimicrob Agents Chemother

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The objective of this study was to assess the antimicrobial resistance of enteroaggregative Escherichia coli (EAEC) and enterotoxigenic E. coli (ETEC) strains causing traveler’s diarrhea (TD) and to investigate the molecular characterization of antimicrobial resistance genes to third-generation cephalosporins, cephamycins, and quinolones. Overall, 39 EAEC and 43 ETEC clinical isolates were studied. The susceptibilities of EAEC and ETEC against ampicillin, amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, azithromycin, and rifaximin were determined. All genes encoding resistance determinants were detected by PCR or PCR plus DNA sequencing. The epidemiology of selected EAEC and ETEC strains was studied using multilocus sequence typing (MLST). The resistance to quinolones of EAEC and ETEC strains causing TD has significantly increased over the last decades, and high percentages have been found especially in patients traveling to India and sub-Saharan Africa. Sequence type 38 (ST38) and ST131, carrying the blaCTX-M-15 and blaCTX-M-27 genes, respectively, are highly prevalent among extended-spectrum β-lactamase (ESBL)-producing EAEC and ETEC strains. The cephamycinase ACT-20 is described in the present study for the first time in EAEC and ETEC strains causing TD in patients who had traveled to Central America. The percentages of resistance to azithromycin in EAEC and ETEC isolates from patients to Southeast Asia/India and Africa are above 25%. Meanwhile, rifaximin is still active against EAEC and ETEC, with the prevalence of resistant strains not being high. In conclusion, fluoroquinolones should no longer be considered the drugs of choice for the prevention or treatment in TD for travelers traveling to India and Africa. Azithromycin and rifaximin are still a good alternative to treat TD caused by EAEC or ETEC.

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Section: Methodsmentioning

confidence: 99%