Multiplex sample NMR: an approach to high-throughput NMR using a parallel coil probe

MacNamara, Ernesto; Hou, Ting; Fisher, G. W.; Williams, Sheldon; Raftery, Daniel

doi:10.1016/s0003-2670(99)00387-6

Cited by 62 publications

(58 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, efforts should be made to speed up and optimize the automation processes for further increasing the throughput. In addition, owing to the high sensitivity of the method, it would be possible to increase the throughput by performing simultaneous data collection on multiple samples using capillary lines formed into a bundle that is 5 mm in diameter [Ross and Senn, 2001], or using a four-coil, flow-through multiplex sample probe [MacNamara et al, 1999].…”

Section: (X4)-fabsmentioning

confidence: 99%

Rapid NMR-based functional screening and IC50 measurements performed at unprecedentedly low enzyme concentration

et al. 2005

View full text Add to dashboard Cite

NMR-based functional screening performed with 3-FABS represents a reliable method for identifying inhibitors and measuring with high accuracy their inhibitory activity. Although the method scores high in quality, in its current status it cannot compete with the very high sensitivity of the fluorescence-based techniques. In order to perform ultra-high-throughput screening and to use the small volumes that are possible with fluorescence-based techniques, it would be necessary to further improve the sensitivity of 3-FABS. Two variants of the original experiment with improved sensitivity, namely the 3(x2)-FABS and the 3(x4)-FABS, are presented. The substrate for this purpose is labelled with two and four magnetically equivalent CF 3 moieties. Chemical synthesis of novel fragments containing poly CF 3 moieties and application of the 3-FABS variants to the rapid screening of the Ser/Thr kinase PKA and the protease trypsin are presented. The high sensitivity of the methods allows performing rapid NMR-based functional screening at an unprecedented low enzyme concentration. Drug Dev Res 64: 105-113, 2005. c 2005 Wiley-Liss, Inc.

show abstract

Section: (X4)-fabsmentioning

confidence: 99%

Rapid NMR-based functional screening and IC50 measurements performed at unprecedentedly low enzyme concentration

et al. 2005

View full text Add to dashboard Cite

show abstract

“…However, owing to the long acquisition times required for the NOE experiments, separate CapLC-NMR runs were used to obtain data on each metabolite, so the total volume of urine required was therefore 120 µl. It should be noted that either off-line collection of the peaks or analysis in a multi-coil probe 24,25 could alleviate this need for extra sample, although the off-line approach would inevitably lead to sample losses.…”

Section: 23mentioning

confidence: 99%

A comparison of capillary‐scale LC–NMR with alternative techniques: spectroscopic and practical considerations

Lewis

Bernstein

Duncan

et al. 2005

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

Experimental and practical details for the use of capillary LC (CapLC)-NMR are reported. The capillary NMR probe has high sensitivity and excellent flow characteristics and we found CapLC-NMR to be best suited to samples that are truly mass limited. CapLC-NMR relies on good capillary-scale chromatography where highly concentrated peaks with a volume closely matched to the NMR flow cell are achievable. Provided that the loading capacity of the capillary column is not limiting, the combination of high sensitivity and high solvent suppression quality makes CapLC-NMR an excellent choice. For many real samples, however, the loading is limiting and we found the combination of LC-SPE-MS-NMR with a cryoprobe enables more material to be purified for NMR analysis, while retaining sensitivity.

show abstract

“…For microfluidics the direct sensitivity is less than 10 À4 of traditional high-resolution NMR under these conditions. While several methods have been developed to deal with ultrasmall-samples such as microsolenoid RF coils for NMR, [8][9][10] and magnetic resonance force microscopy (MRFM), 11 to name a few, this review is concerned only with those techniques compatible with conventional planar microfluidic devices of arbitrary channel geometry under standard operating conditions. This requirement limits the discussion to those methods in which any location of the device can be analyzed either spectroscopically or through imaging without making physical contact with the sample such as necessary with force detection.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance detection: spectroscopy and imaging of lab-on-a-chip

Harel

2009

Lab Chip

View full text Add to dashboard Cite

This mini-review is focused on the use of nuclear magnetic resonance (NMR) spectroscopy and imaging to study processes on lab-on-a-chip devices. NMR as an analytical tool is unmatched in its impact across nearly every area of science, from biochemistry and medicine to fundamental chemistry and physics. The controls available to the NMR spectroscopist or imager are vast, allowing for everything from high level structural determination of proteins in solution to detailed contrast imaging of organs in-vivo. Unfortunately, the weak nuclear magnetic moment of the nucleus requires that a very large number of spins be present for an inductively detectable signal, making the use of magnetic resonance as a detection modality for microfluidic devices especially challenging. Here we present recent efforts to combat the inherent sensitivity limitation of magnetic resonance for lab-on-a-chip applications. Principles and examples of different approaches are presented that highlight the flexibility and advantages of this type of detection modality.

show abstract

Multiplex sample NMR: an approach to high-throughput NMR using a parallel coil probe

Cited by 62 publications

References 22 publications

Rapid NMR-based functional screening and IC50 measurements performed at unprecedentedly low enzyme concentration

Rapid NMR-based functional screening and IC50 measurements performed at unprecedentedly low enzyme concentration

A comparison of capillary‐scale LC–NMR with alternative techniques: spectroscopic and practical considerations

Magnetic resonance detection: spectroscopy and imaging of lab-on-a-chip

Contact Info

Product

Resources

About