Multiplex RT‐PCR assay for the detection of major fusion transcripts in Taiwanese children with B‐lineage acute lymphoblastic leukemia

Liang, Der Cherng; Shih, Lee Yung; Yang, Chao; Hung, Iou‐Jih; Chen, Shu Huey; Jaing, Tang Her; Liu, Hsi‐Che; Chang, Wan Hui

doi:10.1002/mpo.10092

Cited by 25 publications

(20 citation statements)

References 28 publications

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“…Taken together, the frequency of Ras mutations in the current series was 20.8% in children with B-precursor ALL, which comprised of 86% of patients with childhood ALL in Taiwan. 26 Lü bbert et al reported that 6 of 100 children (6%) with ALL carried N-Ras mutations, 15 but none had K-Ras mutations. Yokota et al observed that 14 of 125 Japanese children (11%) with ALL had N-Ras mutations that were not correlated with outcome.…”

Section: Discussionmentioning

confidence: 99%

K‐ras mutations and N‐ras mutations in childhood acute leukemias with or without mixed‐lineage leukemia gene rearrangements

Liang

Shih

et al. 2006

Cancer

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Objective Juvenile localized scleroderma (LS) is a chronic inflammatory skin disorder associated with substantial morbidity and disability. Although a wide range of therapeutic strategies has been reported in the literature, a lack of agreement on treatment specifics and accepted methods for clinical assessment has made it difficult to compare approaches and identify optimal therapy. Our objective was to develop standardized treatment plans, clinical assessments, and response criteria for active, moderate to high severity juvenile LS. Methods A core group of pediatric rheumatologists, dermatologists, and a lay advisor was engaged by the Childhood Arthritis and Rheumatology Research Alliance (CARRA) to develop standardized treatment plans and assessment parameters for juvenile LS using consensus methods/nominal group techniques. Recommendations were validated in 2 face‐to‐face conferences with a larger group of practitioners with expertise in juvenile LS and with the full membership of CARRA, which encompasses the majority of pediatric rheumatologists in the US and Canada. Results Consensus was achieved on standardized treatment plans that reflect the prevailing treatment practices of CARRA members. Standardized clinical assessment methods and provisional treatment response criteria were also developed. Greater than 90% of pediatric rheumatologists responding to a survey (66% of CARRA membership) affirmed the final recommendations and agreed to utilize these consensus plans to treat patients with juvenile LS. Conclusion Using consensus methodology, we have developed standardized treatment plans and assessment methods for juvenile LS. The high level of support among pediatric rheumatologists will support future comparative effectiveness studies and enable the development of evidence‐based guidelines for the treatment of juvenile LS.

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Section: Discussionmentioning

confidence: 99%

K‐ras mutations and N‐ras mutations in childhood acute leukemias with or without mixed‐lineage leukemia gene rearrangements

Liang

Shih

et al. 2006

Cancer

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“…Leukemic cells were classified as B-lineage or T-lineage and further by cytogenetic evaluation and/or molecular analysis, performed as described earlier. 4 All protocols were approved by the Institutional Review Boards of the participating institutions. Written informed consent was obtained for all patients.…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, the frequency of ETV6-RUNX1 fusion in Taiwan, 18-19% in B-lineage ALL, has been consistently lower than that (B25%) in western countries. 4,22 In Japan, the frequency of ETV6-RUNX1 fusion in children with ALL seems to be even lower than in Taiwan (13%). 23 The frequency of hyperdiploidy with a modal chromosome number of 51-68 is also lower (11.9%) in childhood ALL cases in Taiwan than in western countries (25-27%).…”

Section: -Year Efs (%)mentioning

confidence: 99%

Long-term results of Taiwan Pediatric Oncology Group studies 1997 and 2002 for childhood acute lymphoblastic leukemia

et al. 2009

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The long-term outcome of 1390 children with acute lymphoblastic leukemia (ALL), treated in two successive clinical trials (Taiwan Pediatric Oncology Group (TPOG)-ALL-97 and TPOG-ALL-2002) between 1997 and 2007, is reported. The event-free survival improved significantly (P ¼ 0.0004) over this period, 69.3±1.9% in 1997-2001 to 77.4±1.7% in 2002-2007. A randomized trial in TPOG-97 testing L-asparaginase versus epidoxorubicin in combination with vincristine and prednisolone for remission induction in standard-risk (SR; low-risk) patients yielded similar outcomes. Another randomized trial, in TPOG-2002, showed that for SR patients, two reinduction courses did not improve long-term outcome over one course. Decreasing use of prophylactic cranial irradiation in the period 1997-2008 was not associated with increased rates of CNS relapse, prompting complete omission of prophylactic cranial irradiation from TPOG protocols, beginning in 2009. Decreased use of etoposide and cranial irradiation likely contributed to the low incidence of second cancers. High-risk B-lineage ALL, T-cell, CD10 negativity, t(9;22), infant, and higher leukocyte count were consistently adverse factors, whereas hyperdiploidy 450 was a consistently favorable factor. Higher leukocyte count and t(9;22) retained prognostic significance in both TPOG-97 and TPOG-2002 by multivariate analysis. Although long-term outcome in TPOG clinical trials is comparable with results being reported worldwide, the persistent strength of certain prognostic variables and the lower frequencies of favorable outcome predictors, such as ETV6-RUNX1 and hyperdiploidy 450, in Taiwanese children warrant renewed effort to cure a higher proportion of patients while preserving their quality of life.

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“…The mononuclear cells were isolated from bone marrow samples and cryopreserved until use. Standard morphological classification, immunophenotyping, and cytogenetic analysis were performed at initial diagnosis as described previously [8]. CNS leukemia was defined as the presence of leukemic cells on a cytocentrifuged preparation of cerebrospinal fluid in conjunction with ≥5 WBC/L.…”

Section: Patients and Samplesmentioning

confidence: 99%

Clinical features, molecular diagnosis, and treatment outcome of infants with leukemia in Taiwan

Chen

Yang

Hung

et al. 2010

Pediatric Blood & Cancer

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Background. Infant leukemia is rare and quite distinct from other childhood leukemias. Differentiating between leukemia and transient myeloproliferative disorder (TMD) in phenotypically normal infants is sometimes difficult. The clinical features and molecular analyses for the fusion transcripts of mixed lineage leukemia (MLL) gene rearrangement in infant leukemia have not been well documented in the Chinese population. Procedure. Forty-five consecutive infants diagnosed with leukemia between 1995 and 2007 in a tertiary medical center in Taiwan were studied. Acute lymphoblastic leukemia (ALL) was diagnosed in 23 infants, acute myeloid leukemia (AML) in 21 (including TMD in 4), and juvenile myelomonocytic leukemia (JMML) in 1. Results. The median white count at diagnosis was higher in ALL than in AML (154.4 × 10 9 /l vs. 58.3 × 10 9 /l, P = 0.05). Chromosome 11q23/MLL abnormalities were present in 77% of ALL and 31% of AML. The 5-year event-free survival (EFS) in infant ALL and AML showed no difference (18% vs. 12%, respectively). The only independent predictor of an adverse prognosis among infants diagnosed with ALL was high presenting white count ≥ 100 × 10 9 /l (P = 0.05). However, no factor was associated with an adverse outcome for infants with AML. Conclusions. The molecular assessments and prognostic factors of infant leukemia in Taiwan mirror those in developed Western countries. Continued molecular investigations and development of more effective therapies are needed.

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Multiplex RT‐PCR assay for the detection of major fusion transcripts in Taiwanese children with B‐lineage acute lymphoblastic leukemia

Abstract: Multiplex RT-PCR assay is an efficient, sensitive, accurate, and cost-effective diagnostic tool, which will likely improve our ability in accurately and rapidly risk-stratifying children with ALL.

Cited by 25 publications

References 28 publications

K‐ras mutations and N‐ras mutations in childhood acute leukemias with or without mixed‐lineage leukemia gene rearrangements

K‐ras mutations and N‐ras mutations in childhood acute leukemias with or without mixed‐lineage leukemia gene rearrangements

Long-term results of Taiwan Pediatric Oncology Group studies 1997 and 2002 for childhood acute lymphoblastic leukemia

Clinical features, molecular diagnosis, and treatment outcome of infants with leukemia in Taiwan

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