Multiplex polymerase chain reaction testing in pediatric inpatients with febrile seizures

Meyer, Sascha; Rissland, Jürgen; Simon, Arne; Poryo, Martin; Gortner, Ludwig; Naric, Jelena

doi:10.1016/j.jpeds.2016.07.047

Cited by 3 publications

(2 citation statements)

References 7 publications

(3 reference statements)

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“…Copan swabs in universal transport medium were sent by a pneumatic delivery system to the related Institute of Virology. There, a multiplex PCR was performed (FTD Respiratory pathogens 21, Fast Track Diagnostics – A Siemens Healthineers Company) as described previously 17 18 . Positive results were reported to the treating physicians normally within 6 to 12 hours.…”

Section: Methodsmentioning

confidence: 99%

Inpatient Rsv-Management 2016–2022: Epidemiology and Adherence to A Bronchiolitis Treatment Standard at a German University Children’s Hospital

Heidtmann,

Baltaci,

Meyer

et al. 2024

Klin Padiatr

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Background This study analyzes the RSV season 2021/2022 in a referral children’s hospital, compares the epidemiology and illness severity with RSV-infected inpatients from 2016 to 2020 and audits the adherence to our internal therapy standard for RSV bronchiolitis. Material and methods Inpatients with rtPCR-confirmed RSV infection (Jan. 2016 to Jan. 2022). Results The audit comprises 306 RSV inpatients, on average 50 hospitalizations per year; in 03/2020, a rapid RSV Season-Offset was observed. In the winter season 2020/2021, no patient with RSV was hospitalized. Beginning in July, we noticed a rapid increase of RSV-admissions (most cases in Sept./Oct, duration until Dec. 2021; n=53). In 2021–2022, a significant larger share needed PICU admission (9.4% vs 3.2%, p=0.040). Adherence to the internal guidance was low; only 11.8% (n=36) of all patients received supportive treatment without inhalative or systemic medications, 37% of all patients received antibiotics. Conclusions This audit confirms the strong impact of public preventive measures directed against SARS-CoV-2 transmission on RSV epidemiology. Few weeks after easing public COVID-19 restrictions (summer 2021), RSV inpatient cases rapidly increased, lasting until Dec. 2021. The audit of bronchiolitis management revealed surprisingly low adherence to the internal guidance, despite a face-to-face educational session with the attending pediatricians in Oct. 2021. Low adherence resulted in an unnecessary exposure of RSV patients to systemic medications of questionable benefit including antibiotics.

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Section: Methodsmentioning

confidence: 99%

Inpatient Rsv-Management 2016–2022: Epidemiology and Adherence to A Bronchiolitis Treatment Standard at a German University Children’s Hospital

Heidtmann,

Baltaci,

Meyer

et al. 2024

Klin Padiatr

Self Cite

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“…The multiplex panel (FTD Respiratory pathogens 21, Siemens Healthineers) included the following pathogen detections: influenza A; influenza A/ H1N1; influenza B; parainfluenza types 1, 2, 3, 4; coronavirus NL63, 229E, OC43, HKU1; human metapneumovirus (A/B); human bocavirus; rhinovirus; adenovirus; respiratory syncytial virus (A/B); parechovirus; enterovirus and Mycoplasma pneumoniae. This commercial, real-time PCR assay was performed according to the manufacturer's instructions and as previously described [17]. Bacterial infections were confirmed by bacteriologic cultures of blood, throat swabs, or other clinical samples.…”

Section: Methodsmentioning

confidence: 99%

Expression of TRAIL, IP-10, and CRP in children with suspected COVID-19 and real-life impact of a computational signature on clinical decision-making: a prospective cohort study

et al. 2023

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Purpose We evaluated the host-response marker score “BV” and its components TRAIL, IP-10, and CRP in SARS-CoV-2 positive children, and estimated the potential impact on clinical decision-making. Methods We prospectively analyzed levels of TRAIL, IP-10, CRP, and the BV score, in children with suspected COVID-19. Classification of infectious etiology was performed by an expert panel. We used a 5-point-questionnaire to evaluate the intention to treat with antibiotics before and after receiving test results. Results We screened 111 children, of whom 6 (5.4%) were positive for SARS-CoV-2. A total of 53 children were included for the exploratory analysis. Median age was 3.1 years (interquartile range [IQR] 1.3–4.3), and 54.7% (n = 29) were girls. A viral and a bacterial biomarker pattern was found in 27/53 (50.9%) and 15/53 (28.3%), respectively. BV scores differed between COVID-19, children with other viral infections, and children with bacterial infections (medians 29.5 vs. 9 vs. 66; p = 0.0006). Similarly, median TRAIL levels were different (65.5 vs. 110 vs. 78; p = 0.037). We found no differences in IP-10 levels (555 vs. 504 vs. 285; p = 0.22). We found a concordance between physicians’ “unlikely intention to treat” children with a viral test result in most cases (n = 19/24, 79.2%). When physicians expressed a “likely intention to treat” (n = 15), BV test revealed 5 bacterial, viral, and equivocal scores each. Antibiotics were withheld in three cases (20%). Overall, 27/42 (64%) of pediatricians appraised the BV test positively, and considered it helpful in clinical practice. Conclusion Host-response based categorization of infectious diseases might help to overcome diagnostic uncertainty, support clinical decision-making and reduce unnecessary antibiotic treatment.

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Risk factors for acute encephalitis and early seizure recurrence in complex febrile seizures

Kajiwara

Koga

2022

Eur J Pediatr

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Multiplex polymerase chain reaction testing in pediatric inpatients with febrile seizures

Cited by 3 publications

References 7 publications

Inpatient Rsv-Management 2016–2022: Epidemiology and Adherence to A Bronchiolitis Treatment Standard at a German University Children’s Hospital

Inpatient Rsv-Management 2016–2022: Epidemiology and Adherence to A Bronchiolitis Treatment Standard at a German University Children’s Hospital

Expression of TRAIL, IP-10, and CRP in children with suspected COVID-19 and real-life impact of a computational signature on clinical decision-making: a prospective cohort study

Risk factors for acute encephalitis and early seizure recurrence in complex febrile seizures

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