Multiplex Polymerase Chain Reaction in Microsatellite Analysis Urine Sediment Cells. A Fast and Cheap Method in Diagnosing and Monitoring of Superficial Transitional Bladder Cell Carcinoma

Prognostic Role of Loss of Heterozygosity on Chromosome 18 in Patients With Low-Risk Nonmuscle-Invasive Bladder Cancer: Results from a Prospective Study

“…LOH analysis was also performed in blood samples in order to obtain the leukocyte DNA to use as control. Specimen collection, DNA extraction, Chr 18 LOH analysis, and the subsequent data interpretation were carried out by using the same method previously described along with urinary cytology [10,11]. In brief, three analyzed sequences are located on Chr 18 (D18S51, MBP LW, MBP H).…”

“…The microsatellite markers used in the present study and their locations were taken from the Genome Database (http://www.gdb.org). LOH was defined as the complete or nearly complete absence of one allele in tumor DNA [10,11]. LOH analyses were run in duplicate using independent PCR reactions, in order to avoid analysis errors.…”

“…However, recent reports have demonstrated that microsatellite alterations, such as microsatellite instability and/or loss of heterozygosity (LOH) are a common feature of neoplastic cells [9]. For these reasons, microsatellite analysis seems to be an efficacious method with a high potential in predicting bladder cancer recurrence [10,11]. Several authors have reported that the allelic deletion detected as LOH represents an important mechanism in the development and progression of urothelial bladder cancer [12].…”