Multiplex polymerase chain reaction for identification of bovine mastitis associated pathogens

Lamba, Pusha; Purva, Mukul; Meena, Nareshi

doi:10.22271/chemi.2020.v8.i6m.10879

Cited by 1 publication

(1 citation statement)

References 14 publications

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“…The simultaneous identification of Staphylococcus 65 (32.5%), Streptococcus 37 (18.5%) and E. coli 16 (8%) was made possible by multiplexing. However, Choudhary, (2017). evaluated nine different microorganism species using multiplex PCR and found that S. aureus, S. hyicus, S. agalactiae, S. uberis, E. coli, Klebsiella pneumoniae, Listeria monocytogenus, Mycoplasma bovis and Pseudomonas aeruginosa were all currently detectable.…”

Section: Development Of Multiplex Pcrmentioning

confidence: 99%

Molecular Detection of Some Most Important Pathogenic Bacteria by Multiplex PCR for Diagnosis of Subclinical Mastitis in Bovine

Solanki,

Devi

2024

AJDFR

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Background: The dairy sector faces formidable obstacles in the early detection of the bacteria causing subclinical mastitis and in the proactive treatment of those cases. The objective of the study was to access multiplex PCR assays (MPCR) and conventional methods for the concurrent identification of significant bacteria that cause sub-clinical mastitis and to compare these methods. 200 samples of pooled milk from cows and buffaloes in Rajasthan were gathered between 2020 and 2021. Methods: The primary bacterial pathogens in milk samples were identified using conventional methods such multiplex PCR, biochemical testing and culture. Result: From 200 combined samples of cow and buffalo milk, the traditional approach was able to extract 97 different strains. Staphylococcus aureus 54 (27%), Streptococcus spp. 30 (15%) and E. coli 13 (6.5%) were shown to be prevalent as single or mixed infections, respectively. Staphylococcus was the main pathogen that was discovered. concurrently, S. aureus, Streptococcus and E. coli. Direct detection of Staphylococcus 65 (32.5%), Streptococcus 37 (18.5%), E. coli and 16 (8%) by multiplex PCR was found in milk samples. The analysis revealed that because multiplex PCR assays have higher specificity and sensitivity than conventional procedures, they are more reliable. The multiplex PCR method employed in the current study was a simple and quick technique to identify the major pathogens and it has the potential to be a very helpful tool for determining the pathogens that cause environmental mastitis and evaluating the health of the herd.

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Section: Development Of Multiplex Pcrmentioning

confidence: 99%