Multiplex polymerase chain reaction-based analysis of T-cell receptor ? gene rearrangements for the determination of T-lymphocyte clonality

Molecular analysis of hprt mutant lymphocytes from 1,3‐butadiene–exposed workers

“…A 12-to 15-l aliquot of the PCR product was analyzed by electrophoresis on 3% NuSieve 3:1 agarose gel stained with ethidium bromide. The T-cell receptor (TCR) ␥ gene was used as a control to check the DNA quality [Ma et al, 2000].…”

“…The remaining mutants were not characterized because of either poor RNA quality (54 mutants) or failure in cDNA synthesis or sequence analysis (30 mutants). When identical mutations were observed in separate mutant clones isolated from an individual, we analyzed the cells for TCR ␥ gene rearrangements to determine whether they derived from the in vivo clonal expansion of a single mutated cell [Ma et al, 2000]. The results of the sequence analysis of the human hprt gene in mutant T-lymphocytes from BD-exposed workers and control subjects are summarized in Table IV.…”

Acetaldehyde as a Food Flavoring Substance: Aspects of Risk Assessment

“…After a single i.p. administration, acetaldehyde was found to be genotoxic as evidenced by a significant increase in sister‐chromatid exchanges in the bone marrow of mice and hamsters (at ≥0.5 mg acetaldehyde kg −1 bw), and positive findings in the mouse [ 147,148 ] and rat micronucleus test (bone marrow and peripheral blood; at ≥190 mg acetaldehyde kg −1 bw, 24 h) ( Table 5 ). Further information on toxicity/mortality was not provided in some of these studies and the purity of the test substance was sometimes not reported or low (89.4%).…”

“…Further information on toxicity/mortality was not provided in some of these studies and the purity of the test substance was sometimes not reported or low (89.4%). [ 147‐149 ] Acetaldehyde was negative in the micronucleus test with mouse spermatids (125–500 mg kg −1 bw i.p.). [ 150 ]…”