Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation

Schreiner, Tamara; Ronzheimer, A.; Friz, M.; Morlock, Gertrud E.

doi:10.1016/j.foodchem.2022.133610

Cited by 20 publications

(17 citation statements)

References 43 publications

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“…This complex interplay of substances responsible for such effects may not only exhibit positive effects, but may also compensate for, attenuate or cancel out possible undesirable effects of other components ( Caesar and Cech, 2019 ). The recent combination of planar chromatography with multiplex biological effect detection can straightforwardly differentiate such effects on the same plate ( Ronzheimer et al, 2022 ; Schreiner et al, 2022 ). In addition, unlike isolated individual substances, MOCS often exhibit a broad spectrum of action ( i.e ., they are multi-target drugs), since the diversity of molecular structures (multi-component), with their specific functional moieties and their respective chemical and physiological properties do not only have one common target, i.e .…”

Section: Inherent Complexitymentioning

confidence: 99%

“…This seems to be another reason why biologically active mono-substances are often favored. When mono-substances are used, the potential of synergistic action is lost, which has recently been discovered in a number of natural MOCS ( Ronzheimer et al, 2022 ; Schreiner et al, 2022 ). However, rising costs for mono-compound isolation and purification increasingly question the positive economic balance, especially in light of the growing awareness for the ecological impact and its ramifications.…”

Section: Inherent Complexitymentioning

confidence: 99%

“…Different molecules and their active groups thus enhance their properties when applied in combination. Nevertheless, possible synergism between individual compounds of an EO is complex and cannot be limited exclusively to a few major constituents ( Ronzheimer et al, 2022 ; Schreiner et al, 2022 ). Thirdly, antagonistic effects may be observed which is the opposite of synergy.…”

Section: Interactions Between Eo Compounds – Safety Assessmentmentioning

confidence: 99%

“…The study results of studies on individual compounds show how precisely adjusted the compounds in their respective composition may act and justify the assumed multi-target mechanisms of action. Of course, it will be necessary to adapt existing methods for the investigation of complex mixtures or to develop completely new experimental procedures to meet the requirements of complexity ( Ronzheimer et al, 2022 ; Schreiner et al, 2022 ). Our current scientific techniques seem to have reached a limit.…”

Section: Antibiotic Resistance and Eos As Potential Answersmentioning

confidence: 99%

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Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and Well-Being

et al. 2022

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Essential oils (EOs) and their individual volatile organic constituents have been an inherent part of our civilization for thousands of years. They are widely used as fragrances in perfumes and cosmetics and contribute to a healthy diet, but also act as active ingredients of pharmaceutical products. Their antibacterial, antiviral, and anti-inflammatory properties have qualified EOs early on for both, the causal and symptomatic therapy of a number of diseases, but also for prevention. Obtained from natural, mostly plant materials, EOs constitute a typical example of a multicomponent mixture (more than one constituent substances, MOCS) with up to several hundreds of individual compounds, which in a sophisticated composition make up the property of a particular complete EO. The integrative use of EOs as MOCS will play a major role in human and veterinary medicine now and in the future and is already widely used in some cases, e.g., in aromatherapy for the treatment of psychosomatic complaints, for inhalation in the treatment of respiratory diseases, or topically administered to manage adverse skin diseases. The diversity of molecules with different functionalities exhibits a broad range of multiple physical and chemical properties, which are the base of their multi-target activity as opposed to single isolated compounds. Whether and how such a broad-spectrum effect is reflected in natural mixtures and which kind of pharmacological potential they provide will be considered in the context of ONE Health in more detail in this review.

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Section: Inherent Complexitymentioning

confidence: 99%

Section: Inherent Complexitymentioning

confidence: 99%

Section: Interactions Between Eo Compounds – Safety Assessmentmentioning

confidence: 99%

Section: Antibiotic Resistance and Eos As Potential Answersmentioning

confidence: 99%

See 2 more Smart Citations

Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and Well-Being

et al. 2022

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“…At present, no one can answer what most of these substance signals mean because they are mostly unidentified signals . In addition, there are no data available on the toxicological potential of each detected signal, and the in vitro assays (which only give a sum parameter result) have proven to be misleading regarding opposite effect results in complex samples. , Since clean water is becoming increasingly rare (), the challenge is evident. Plant-based food and other environmental samples are expected to be even more complex than water.…”

mentioning

confidence: 99%

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

et al. 2022

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A complete recipe for building your own chromatography equipment from readily available materials is introduced. It combines sample separation (chemistry laboratory) with biological effect detection (biology laboratory). This hyphenation of two disciplines is necessary for prioritizing important compounds in complex samples. Among the thousands of compounds therein, it is often not clear which compounds are the important ones. On the same separation surface, additional detection of biological effects enables and guides substance prioritization. The newly developed open-source 2LabsToGo system for chemical and biological analysis is completely solvent-resistant and, due to miniaturization, environmentally friendly regarding the consumption of materials. It produces comparable results but is 10 times more compact (26 cm × 31 cm × 34 cm), 10 times lighter (6.8 kg), and 55 times less expensive (€ 1717) than current sophisticated commercial devices. As a proof of concept of the first 2LabsToGo system, the quality of different water samples was analyzed since clean water is becoming increasingly rare. In water, most of the thousands of substance signals or features can neither be identified nor classified toxicologically. However, methods that exploit this hyphenated strategy provide answers to such essential safety issues. Drinking or tap water did not show bioactive or toxic compounds, which was expected, whereas biogas or landfill water samples did. The hyphenated 2LabsToGo strategy is affordable and extremely useful for all laboratories with limited equipment but pressing challenges. It is ready to be used in various analytical tasks and applications.

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Sample preparation for planar chromatography

Poole

2023

J of Separation Science

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High‐performance thin‐layer chromatography has favorable properties for high‐throughput separations with a high matrix tolerance. Sample preparation, however, is sometimes required to control specific matrix interferences and to enhance the detectability of target compounds. Trends in contemporary applications have shifted from absorbance and fluorescence detection to methods employing bioassays and mass spectrometry. Traditional methods (shake‐flask, heat at reflux, Soxhlet, and hydrodistillation) are being challenged by automated instrumental approaches (ultrasound‐assisted and microwave‐assisted solvent extraction, pressurized liquid extraction, and supercritical fluid extraction) and the quick, easy cheap, efficient, rugged, and safe extraction method for faster and streamlined sample processing. Liquid‐liquid extraction remains the most widely used approach for sample clean‐up with increasing competition from solid‐phase extraction. On‐layer sample, clean‐up by planar solid‐phase extraction is increasingly used for complex samples and in combination with heart‐cut multimodal systems. The automated spray‐on sample applicator, the elution head interface, biological detection of target and non‐target compounds, and straightforward mass spectrometric detection are highlighted as the main factors directing current interest toward faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants requiring high concentration factors.

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Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation

Cited by 20 publications

References 43 publications

Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and Well-Being

Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and Well-Being

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

Sample preparation for planar chromatography

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