Multiplex PCR from Menstrual Blood: A Non-Invasive Cost-Effective Approach to Reduce Diagnostic Dilemma for Genital Tuberculosis

Paine, Suman Kalyan; Basu, Analabha; Choudhury, Rajib Gon; Bhattacharya, Basudev; Chatterjee, Sidhartha; Bhattacharya, Chandra

doi:10.1007/s40291-018-0322-3

Cited by 9 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reciprocally, Paine et al . (2018) demonstrated high sensitivities of 95·8% and 90·2% by M‐PCR ( hsp65 + dnaJ + IS 6110 ) within EBs and menstrual blood, respectively, but lesser specificities of 84·3–86·1% were attained, with respect to culture. It is not worthwhile to compare the sensitivity acquired by M‐PCR within menstrual blood specimens with that of Paine et al .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of urogenital tuberculosis by multiplex-nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert® MTB/RIF

Kamra

Alam

Singh

et al. 2022

Letters in Applied Microbiology

View full text Add to dashboard Cite

Significance and Impact of the Study: We developed a multiplex-nested PCR (M-nested PCR) assay targeting mpt64 (Rv1980c) and IS6110 for the diagnosis of male/female urogenital tuberculosis (UGTB) patients, which demonstrated good sensitivity and specificity. Notably, M-nested PCR exhibited significantly higher sensitivities, as compared to M-PCR (mpt64 + IS6110) and GeneXpertâ MTB/RIF assay. After validating these data with the larger sample size, our M-nested PCR may develop into a diagnostic kit for routine use.

show abstract

Section: Resultsmentioning

confidence: 99%

“…2017; Paine et al . 2018). GeneXpert® MTB/RIF assay, a hemi‐nested real‐time PCR targeting rpoB (Rv0664) and using molecular beacons has been a major breakthrough in TB diagnostics including UGTB, which simultaneously detects Mtb and rifampin (RIF) resistance within 2 h (Kamra and Mehta 2021; Dahiya et al .…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of urogenital tuberculosis by multiplex-nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert® MTB/RIF

Kamra

Alam

Singh

et al. 2022

Letters in Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Three sets of primers were used in multiplex PCR and details of primers sequence are described in elsewhere and here we used two type of primers for identification of mycobacteria strain because IS6110 insertion element was absent in the genome of mycobacteria. Most of these strains appeared to be isolated from Vietnamese people as well as north east origin [10]. So IS 6110 insertion element may be limited some certain population but our multiplex system is capable to identify the mycobacteria strain.…”

Section: Multiplex-pcrmentioning

confidence: 97%

“…The efficiency of such test is also of optimal value [10]. Even without a direct involvement of ovaries, the ovarian function, especially the endrocrinological reserve is affected in latent TB cases [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Female Genital Tubercular Involvement on Anti-Mullerian Hormone Secretion in Latent Genital Tuberculosis Affected Women

Chatterjee¹,

Mission²

2019

RMGO

View full text Add to dashboard Cite

show abstract

“…Since the collection of menstrual blood is a noninvasive procedure, M-PCR targeting mpt64+IS6110 has been devised within menstrual blood specimens and compared results with EBs [12], where menstrual blood revealed better sensitivity than EBs. Similarly, a comparison was made between the menstrual blood and EB specimens by M-PCR using IS6110+dnaJ (Rv0352)+hsp65 targets [13], which showed lesser sensitivity but higher specificity with menstrual blood, while the reverse was true with EBs. The utility of M-PCR targeting hsp65+esat6+its (16S-23S internal transcribed spacer sequence) for differential detection of M. tuberculosis and M. avium complex from the suspected female UGTB cases has been documented, which exhibited better sensitivity than laparoscopy/hysteroscopy [4].…”

Section: Naatsmentioning

confidence: 99%

Recent trends in diagnosis of urogenital tuberculosis

Mehta

Kamra

2020

Future Microbiology

View full text Add to dashboard Cite

the efficient diagnosis of UGTB by reliable tests would certainly reduce the infertility in both men and women and the other sequelae associated with disease. " Urogenital tuberculosis (UGTB) is the second most common form of extrapulmonary TB (EPTB) in developing nations with severe epidemic situations. For example, India (comprising 45% of EPTB cases) and the third most common form in locations with a lower incidence of TB [1,2], which include both renal and genital TB. However, concomitant UGTB and pulmonary TB (PTB) are found in 2-10 and 15-20% of patients in developed and developing nations, respectively [2]. In fact, UGTB occurs after a prolonged period of latent infection followed by hematogenous spread to the kidneys, fallopian tubes and epididymis [3]. The clinical manifestations of female UGTB include primary or secondary infertility, menstrual dysfunction and pelvic inflammatory disease [4]. Infertility is the most common complication, which occurs in 5-16% of Indian women caused by irreversible damage to the fallopian tubes. Mostly, fallopian tubes are associated with congestion and miliary tubercles, while endometrium is linked with caseation and ulceration in 50-80% cases, resulting in Asherman's syndrome [5]. Similarly, the most frequent sites for male UGTB are the epididymis and prostate followed by seminal vesicles and testicles originating from renal foci, which also lead to infertility [6]. Owing to asymptomatic nature and varied clinical presentations (ranging from vague urinary symptoms to chronic kidney disease) that mimic several urologic and genital diseases, diagnosis of UGTB is a daunting challenge [3].Smear/culture, histopathology, interferon-γ release assays (IGRAs), intravenous pyelography, laparoscopy and nucleic acid amplification tests (NAATs) are the main modalities employed in the diagnosis of UGTB [6][7][8], which have certain limitations. Smear microscopy and culture for Mycobacterium tuberculosis identification (on Lowenstein-Jensen medium or BACTEC-460/MGIT-960) are commonly used methods but they lead to poor sensitivities due to paucibacillary nature of specimens [7]. Though urine culture is considered the gold standard, it requires skillful technicians and has a prolonged turnaround time of approximately 6-8 weeks [7] when irreparable damage to the fallopian tubes and epididymis already occurs. Delayed diagnosis also leads to nonfunctioning unilateral kidney, contracted bladder and renal failure [1]. Endoscopic procedures such as laparoscopy/hysteroscopy are often linked with operative risks and mostly cause flaring of infection [5], while histopathological examination can be confirmatory only when it demonstrates acid-fast bacilli in the tissues that are very scarce in endometrial biopsies (EBs) [7]. Intravenous pyelography may be suggestive of UGTB but it is nonspecific and often mimics other pathologic lesions [1]. IGRAsImmunological procedures such as IGRAs are widely employed for the early detection of UGTB cases. Notably, Kim et al. [8] reported a sensitivity of 52.6%...

show abstract

Multiplex PCR from Menstrual Blood: A Non-Invasive Cost-Effective Approach to Reduce Diagnostic Dilemma for Genital Tuberculosis

Abstract: A strong clinical suspicion aided with multiplex PCR using menstrual blood may significantly reduce the diagnostic dilemma for GTB diagnosis in a non-invasive, sensitive, rapid, and cost-effective manner.

Cited by 9 publications

References 12 publications

Diagnosis of urogenital tuberculosis by multiplex-nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert® MTB/RIF

Diagnosis of urogenital tuberculosis by multiplex-nested PCR targeting mpt64 (Rv1980c) and IS6110: comparison with multiplex PCR and GeneXpert® MTB/RIF

Effect of Female Genital Tubercular Involvement on Anti-Mullerian Hormone Secretion in Latent Genital Tuberculosis Affected Women

Recent trends in diagnosis of urogenital tuberculosis

Contact Info

Product

Resources

About