Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections

Zmantar, Tarek; Chaieb, Kamel; Abdallah, Fethi Ben; Kahla-Nakbi, Amel Ben; Hassen, Assia Ben; Mahdouani, Kacem; Bakhrouf, Amina

doi:10.1007/s12223-008-0055-5

Cited by 39 publications

(36 citation statements)

References 26 publications

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“…Also, this study found that efflux genes such as msrA gene was present either alone or in association with other genes (23.7%), while the mefA gene was absent from all tested isolates. These results concur with those described by Zmantar et al [35], who reported the same observation. Furthermore, we found a predominance of ermC gene (27/38) among MLS resistance genes.…”

supporting

confidence: 94%

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Osman

Nasbeh²,

Rafei³

et al. 2015

int. arab. j antimicrob. agents

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Objective: Staphylococcus aureus is one of the most significant pathogens causing significant morbidity and mortality. Moreover, the incidence of MLS S. aureus (resistant at least for one macrolide) infections continues to grow globally. The aim of this study is to examine the expression of resistance of S. aureus isolates to MLS and the prevalence of genes involved in this resistance using PCR. Methods:A total 38 strains of S. aureus MLS-resistant were isolated in the Microbiology Labs at Nini Hospital in North Lebanon. The disk diffusion method was used to determine the phenotype of the MLS resistance. The resistance genes involved were detected by PCR using specific gene primers for ermA, ermB, ermC, msrA, linA, mefA, vat and vgb genes.Results: A total of 55.3% of the isolates were positive for inducible phenotype (iMLS B ), of these 15.8% were positive for the constitutive phenotype (cMLS B ), 23.7% for MS B phenotype and 5.2% for L phenotype. The ermC gene was the most prevalent (52.6%), while ermA, ermB, msrA and linA genes were observed with lower prevalence. A combination of several of these genes was detected,whereas the vgb, vat and mefA genes were not detected in any of the clinical isolates. Conclusion:This study is the first investigated characterization of MLS resistance genes in clinical isolates of S. aureus in Lebanon according to

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supporting

confidence: 94%

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Osman

Nasbeh²,

Rafei³

et al. 2015

int. arab. j antimicrob. agents

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“…In comparison with other studies such as the ones mentioned for each gene in the description above, the current results show that the mentioned genes are identified approximately in a similar number as can be found in literature (Harbarth et al, 2006;Zmantar et al, 2008;Crăciunaş et al, 2010).…”

Section: Resultssupporting

confidence: 87%

“…Even more, the genes mentioned were investigated on isolates from blood and urine specimens in Turkey (Aktas et al, 2007) or from unknown MRSA carriers (Harbarth et al, 2006). Other examples are presented as Crăciunaş et al (2010) investigated mecA, femA, ermA, ermB, ermC on clinical isolates, and Zmantar et al (2008) used for their investigation Staphylococcus aureus strains isolated from auricular infections.…”

Section: Introductionmentioning

confidence: 99%

Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

Pruteanu¹,

Jäntschi

Crăciunaş

2017

Not Sci Biol

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Polymerase chain reaction (PCR) techniques development allows the elaboration of many assays for identification of bacteria's resistance mechanisms to antibiotics. Following this idea, the results of molecular level investigation of bacteria's resistance mechanisms to antibiotics may give many opportunities to find more rapid methods for identifying the genes which are responsible for antibiotic resistance induction. The aim of the current study was to investigate antibiotic resistance genes in Staphylococcus bacteria on molecular level. Among classes of antibiotics, macrolides-lincosamides-streptogramin B (MLSB) and beta-lactams were used. In the proposed study, the bacterial strains were represented by 50 isolates of Staphylococcus. The bacterial strains were analyzed using polymerase chain reaction to identify the nuc, tuf, tst, sea, pathogenic activity genes. Thereafter, the bacteria were tested for ermA, ermB, ermC genes and for mecA, femA which are involved in the resistance to macrolides, lincosamides, streptogramin B and to beta-lactams, respectively. The presence or the absence of these genes confirmed that tested strains are resistant to specific antibiotic or not. Bacteria pathogenic activity was emphasized by genes as follows: sea (enterotoxin) which was found at all isolates, tst (toxic shock toxin) gene was not detected in any of the isolates and tuf gene (elongation factor) was obtained with one pair of primers. Resistance to beta-lactams was evidenced by the presence of mecA in all isolates and femA in some strains. Each of ermC, ermA and ermB, macrolides-lincosamides-streptogramin B resistance genes, was detected.

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“…The possible reasons for this type of discrepancy are that the isolates appeared to show poor expression of mecA genes or production of methicillinase (alteration of PBP subtypes) or seem to overproducing β-lactamase. The phenotypic expression of resistance could vary due to growth conditions which are also involved in the expression of methicillin resistance (Zmantar et al, 2008;Turutoglu et al, 2009 andTurkylmaz et al, 2010).…”

Section: Genotypic Identification Of Mrsa Isolatesmentioning

confidence: 99%

Identification and Characterisation of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Mastitis

Bhagat¹,

Kher²,

Dadawala³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections

Cited by 39 publications

References 26 publications

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Characterization of resistance genes to macrolides, lincosamides and streptogramins (MLS) among clinical isolates of Staphylococcus aureus in North Lebanon

Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

Identification and Characterisation of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Mastitis

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