2004
DOI: 10.1007/s00414-003-0419-y
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Multiplex PCR, amplicon size and hybridization efficiency on the NanoChip electronic microarray

Abstract: We tested the SNP typing protocol developed for the NanoChip electronic microarray by analyzing the four Y chromosome loci SRY1532, SRY8299, TAT, and 92R7. Amplicons of different lengths containing the same locus were purified and addressed to the NanoChip array and fluorescently labelled reporter probes were hybridized to the amplicons. We demonstrated that as little as 10-30 fmol of 50 bp DNA amplicons was sufficient to obtain strong and reproducible results. The hybridization to 50 bp amplicons was up to 10… Show more

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Cited by 14 publications
(8 citation statements)
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“…The major detection platform for fragment length analysis of fluorescence-labeled PCR fragments in forensic DNA testing is capillary gel electrophoresis. Supplementary technologies, such as mass spectrometry or microchip devices [23,27], rely on completely different methodologies and equipment and are unlikely to replace capillary gel electrophoresis. In contrast, use of the chemically structured chip presented in this study requires only slight modifications of pre-PCR processing.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…The major detection platform for fragment length analysis of fluorescence-labeled PCR fragments in forensic DNA testing is capillary gel electrophoresis. Supplementary technologies, such as mass spectrometry or microchip devices [23,27], rely on completely different methodologies and equipment and are unlikely to replace capillary gel electrophoresis. In contrast, use of the chemically structured chip presented in this study requires only slight modifications of pre-PCR processing.…”
Section: Discussionmentioning
confidence: 99%
“…Initial developments have been made of lab-on-chip solutions that combine sample preparation, DNA amplification and detection, and determination and visualization of results [26]. Various approaches in (micro)miniaturization of PCR reactions, using microchips, capillaries, and surfaces of microarrays, exist [18,20,[27][28][29]. Electronic microarrays also integrate the amplification and detection of multiple samples [30].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…In particular, Y-chromosome SNPs, because of their abundance, simplicity and low mutation rate, are becoming an extensively used marker set. Forensic laboratories are starting to implement Y chromosome SNP analysis, in order to investigate the forensic usefulness of these markers (Sanchez et al 2003;Borsting et al 2004). …”
Section: Brion · B Sobrino · a Blanco-verea · M V Lareu · A Cmentioning
confidence: 99%
“…Electronic microarrays provide speed, convenience and full automation. It has been used in different applications including on-chip strand displacement amplification Westin et al, 2000Westin et al, , 2001, single nucleotide polymorphism (SNP) and mutation detection (Gilles et al, 1999;Santacroce et al, 2002;Cooper and Goering, 2003;Borsting et al, 2004), gene expression profiling (Weidenhammer et al, 2002), immunoassay (Yang et al, 2002) and simple sequence repeat discrimination (Radtkey et al, 2000).…”
Section: Introductionmentioning
confidence: 99%