Multiplex mRNA profiling for the identification of body fluids

Juusola, Jane; Ballantyne, Jack

doi:10.1016/j.forsciint.2005.02.020

Cited by 292 publications

(207 citation statements)

References 24 publications

(49 reference statements)

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“…organic extraction) as described previously [6] or with column-based kits (RNeasy Mini Kit or AllPrep DNA/RNA Mini Kit, Qiagen, Hombrechtikon, Switzerland and Germantown, MD), according to the manufacturer's protocol, with some adaptations [14]. Casework and environmentally compromised bloodstains were incubated at 56 o C for up to three 7/38 hours in RLTplus buffer for improved extraction and recovery of RNA from challenging samples.…”

Section: Rna Extractionmentioning

confidence: 99%

“…RNA was quantified as described previously [6] or with the Quant-iT RNA assay kit and the Qubit fluorometer (Invitrogen, Basel, Switzerland), according to the manufacturer's protocol.…”

Section: Rna Quantificationmentioning

confidence: 99%

“…Primers for the amplification of gene specific sequences were obtained from published sources [6,13,14,16]. Additional primer sets were designed for HBA, ANK1 and CD3G, using the Primer3 Software (Electronic supplementary data, Table S1).…”

Section: /38mentioning

confidence: 99%

“…A number of mRNA markers have been identified for the forensically most relevant body fluids: blood, saliva, semen, vaginal secretions and menstrual blood [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17]. RNA is notorious for its rapid post-mortem and in vitro decay, but several reports have pointed out an unexpectedly high stability of RNA in forensic stains [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…Blood was chosen for its easy availability, stability and the number of reported markers [6][7][8][9][10][13][14][16][17][19][20]. The following blood markers were included in the study: HBA and HBB are the alpha-and betasubunits of hemoglobin A [21,22]; aminolevulinate synthase 2 (ALAS2) is an erythroid-specific mitochondrial enzyme, that catalyzes the first step in the heme biosynthetic pathway [23]; CD3 gamma molecule (CD3G) is part of the T-cell receptor-CD3 complex, which plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways [24]; ankyrin 1 (ANK1) is an erythrocyte membrane protein, which provides the primary linkage between the membrane skeleton and the plasma membrane [25]; the erythroid form of porphobilinogen deaminase (PBGD) is the third enzyme of the heme biosynthetic pathway [26]; β-spectrin (SPTB) is an erythrocyte membrane protein [27]; Aquaporin (AQP9) is a water channel protein expressed in peripheral leukocytes [28].…”

Section: Introductionmentioning

confidence: 99%

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Selection of highly specific and sensitive mRNA biomarkers for the identification of blood

Haas

Hanson

Krätzer

et al. 2011

Forensic Science International: Genetics

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In the present work, we have evaluated eight reportedly blood-specific mRNA markers (HBB, HBA, ALAS2, CD3G, ANK1, PBGD, SPTB and AQP9) in an attempt to determine the most suitable ones for use in forensic applications based on their sensitivities, specificities and performance with casework samples.While varying levels of expression were observed, all markers were relatively sensitive requiring as little as 1 ng of RNA input into the reverse transcription (RT) reaction. In singleplex reactions, seven of the eight analyzed blood markers (all except AQP9) demonstrated a high degree of specificity for blood. In multiplex reactions, non-reproducible cross-reactivity was observed for several of the mRNA markers, which was reduced and, in most cases, eliminated when less input total RNA was used. Additionally, some cross-reactivity was observed with tissue and animal samples. Despite differences in the observed sensitivity and specificity of the blood markers examined in this study, a number of the candidates appear to be suitable for inclusion in appropriately validated multiplex mRNA-based body fluid identification systems.

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Section: Rna Extractionmentioning

confidence: 99%

“…RNA was quantified as described previously [6] or with the Quant-iT RNA assay kit and the Qubit fluorometer (Invitrogen, Basel, Switzerland), according to the manufacturer's protocol.…”

Section: Rna Quantificationmentioning

confidence: 99%

Section: /38mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Selection of highly specific and sensitive mRNA biomarkers for the identification of blood

Haas

Hanson

Krätzer

et al. 2011

Forensic Science International: Genetics

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Forensic DNA Analysis

Prinz¹,

Lessig²

2014

Handbook of Forensic Medicine

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Current status of mucins in the diagnosis and therapy of cancer

et al. 2009

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Mucins are the most abundant high molecular weight glycoproteins in mucus. Their nature and glycosylation content dictates the biochemical and biophysical properties of viscoelastic secretions, pointing out an important role in diverse biological functions, such as differentiation, cell adhesions, immune responses, and cell signaling. Mucins are expressed in tubular organs by specialized epithelial cells in the body. Their aberrant expression is well documented in a variety of inflammatory or malignant diseases. From a prognosis point of view, their expression and alterations in glycosylation are associated with the development and progression of malignant diseases. Therefore, mucins can be used as valuable markers to distinguish between normal and disease conditions. Indeed, this alteration in glycosylation patterns generates several epitopes in the oligosaccharide side chains that can be used as diagnostic and/or prognostic markers. Furthermore, these characteristic tumor-associated epitopes are extensively used as appropriate immunotargets of malignant epithelial cells. Therefore, in an effort to detect and treat cancer at the earliest stage possible, mucins are analyzed as potential markers of disease for diagnosis, progression, and for therapeutic purposes. In this review, we focused on the current status of the distribution of mucins in normal and pathologic conditions and their clinical use both in cancer diagnosis and therapeutics treatments.

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Multiplex mRNA profiling for the identification of body fluids

Cited by 292 publications

References 24 publications

Selection of highly specific and sensitive mRNA biomarkers for the identification of blood

Selection of highly specific and sensitive mRNA biomarkers for the identification of blood

Forensic DNA Analysis

Current status of mucins in the diagnosis and therapy of cancer

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