Multiplex Immunofluorescence and the Digital Image Analysis Workflow for Evaluation of the Tumor Immune Environment in Translational Research

Abstract

Center for contributing information on mIF and multiplex immunohistochemistry quality and processing. We also thank MD Anderson Editing Services, Research Medical Library.Author Contributions: FR and ERP conceived the idea and theme developed in this article and wrote most of the manuscript. SH, RL, and CL contributed their expertise to digital image analysis and immune profiling. ERP developed the mIF technology in our laboratory and edited the manuscript. All authors approved the final manuscript.

"Let us not Forget Macrophages: The Role of M1 and M2 Macrophages in the Human Body"

“…Further study of macrophages may yield the specific markers or targets that can be exploited to create therapies that are both specific and effective. As new platforms for research emerge, such as spatial transcriptomics [27], protein analysis [28], and even multi-omic platforms [29], and as more robust and widespread platforms such as multiplex immunofluorescence with digital image analysis [30] become available in more countries, we can pave the way to study every facet of disease and the interactions of macrophages in such conditions [31].…”