Multiplex Identification of Gram-Positive Bacteria and Resistance Determinants Directly from Positive Blood Culture Broths: Evaluation of an Automated Microarray-Based Nucleic Acid Test

Buchan, Blake W.; Ginocchio, Christine C.; Manii, Ryhana; Cavagnolo, Robert; Pancholi, Preeti; Swyers, Lettie; Thomson, Richard B.; Anderson, Christopher J.; Kaul, Karen L.; Ledeboer, Nathan A.

doi:10.1371/journal.pmed.1001478

Cited by 113 publications

(102 citation statements)

References 57 publications

(77 reference statements)

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“…The gold standard method for pathogen identification in BSI, used also in our laboratory, requires culturing patient blood in an automated continuous monitoring system, resulted as "not detected" which is three times less, compared with the data, obtained in our hands (4). In regards to the oxacillin resistance, Verigene mecA detection and Vitek-2 correlation was reported to be 97-100% [7,8], which is differs from the results obtained in our study, 75.7%.…”

Section: For Example In 2013 In the Military Medicalcontrasting

confidence: 92%

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Implementation of a Rapid Microarray Assay for the Detection of Gram-Positive, Gram-Negative and Resistance Determinants: Assessing Performance in a Clinical Laboratory in Bulgaria

Savov¹,

Trifonova²,

Todorova³

et al. 2015

JPP

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Two hundred and seven positive blood cultures from two blood culture systems (Bactec and BacTAlert) between May 2012 and February 2014 were enrolled in this study. We present the results of prospectively tested, non-duplicate Gram-positive and Gram-negative organisms tested by the BC-GP (Verigene® Gram-positive) and BC-GN (Gram-negative) Blood Culture Assays (Nanosphere, Inc.) for the first time in Bulgaria. These assays represent new tools for the rapid detection of pathogens and resistance markers in blood stream infections. Results of the Verigene System were compared with conventional testing methods. Of the 207 isolates, 180 (87.0%) were targets on the blood culture panels. Correct identification of Gram-positive organisms was 99.2% and of Gram-negative organisms was 98.2% for organisms that could be detected with BC-GP and BC-GN. Additionally, staphylococci tested for the presence of the mecA gene showed a 75.7% correlation with the cefoxitin test and identification of methicillin-resistance by Vitek 2. ESBLs (extended-spectrum beta-lactamases) for Gram-negative organisms were detected by both methods, showing 100% agreement.

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Section: For Example In 2013 In the Military Medicalcontrasting

confidence: 92%

“…FISH (fluorescent in situ hybridization), PCR, MALDI-TOF and sequencing have been implemented for more than a decade [3][4][5][6]. Micro-array panels for BSI investigation have been recently developed to identify various Gram-positive, Gram-negative and antibiotic resistance targets in blood cultures [5,[7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Implementation of a Rapid Microarray Assay for the Detection of Gram-Positive, Gram-Negative and Resistance Determinants: Assessing Performance in a Clinical Laboratory in Bulgaria

Savov¹,

Trifonova²,

Todorova³

et al. 2015

JPP

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“…One of the most serious medical complications causing significant morbidity and mortality among cancer patients is bacteremia (1,2). Therefore, rapid diagnosis of this kind of bacterial infections can lead to better treatment; as a result, the morbidity and mortality of patients will decrease.…”

Section: Introductionmentioning

confidence: 99%

Survey of Enteric Pathogens Causing Bacteremia in Cancer Patients

Kalantar¹,

Aghabarari²,

Asgari³

et al. 2014

Int J Enteric Pathog

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Background: Symptomatic bacteraemia, is a frequent condition among cancer patients with a significant morbidity and mortality all over the world. Objectives: The aim of this study was to determine the burden of enteric pathogens causing bacteremia among cancer patients. Patients and Methods: Ten ml blood samples were withdrawn from the cancer patients under aseptic conditions. The blood specimens were added to the blood culture bottles and incubated at 37°C. The bacterial isolates from these samples were identified by routine biochemical reactions. Results: During the study period, 68 blood samples from cancer patients were analyzed for bacteremia. Of these patients, six were female (08/82%) and 62 were male (91.18%); with age ranging from under 40 years to 85 years old (mean, 63 years). Gastro-intestinal cancer and cancers of head and neck were the most frequent cancer types in the studied group, accounting for 51 (75%) and 15 (22.1%) cases, respectively. The mean weight of patients was 69.18 Kg (range: 49-100 Kg). Similarly, the mean length of hospital stay was 8 days (range: 4-12 days). Positive blood cultures were detected in only 12 (17.65%) and 11 (91.7%) blood specimens from the Cancer Institute, Tehran, compared with one (08.33%) from Shahid Kamali hospital, Karaj. From these patients, 15 bacteria were isolated; E. coli alone outnumbered other species and accounted for 33.33% of the episodes of bacteremia. Conclusions: In conclusion, our investigation revealed that cancers of GI tract are the most common cancer types causing bacteremia and also we identified that most common bacteria causing bacteremia in Cancer Institute, Tehran and Shahid Kamali Hospital, Karaj, are E. coli and S. aureus

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“…The result can be obtained in ∼2.5 hours with ,5 minutes of hands-on time. 81,82 The BC-GP identifies 13 species/genus, including Staphylococcus spp., Streptococcus spp., Listeria spp., and Enterococcus spp., and three resistance markers (mecA, vanA, and vanB), whereas the BC-GN detects nine bacteria and six antimicrobial resistance genes (CTX-M for the detection of extended-spectrum beta-lactamases and IMP, KPC, NDM, OXA, and VIM for the detection of carbapenemase). [82][83][84] One of the advantages of the assay is that it identifies more rapidly and accurately organisms and their antibiotic resistance genes, compared to traditional methods.…”

mentioning

confidence: 99%

In vitro diagnosis of sepsis: a review

Marcello

Tumolo²,

Donno³

et al. 2016

PLMI

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Sepsis, severe sepsis and septic shock, systemic inflammatory response, and other related manifestations represent a relevant medical problem with high morbidity and mortality, despite the improvements in diagnosis, treatment, and preventive measures over the last few decades. The limited knowledge of the pathophysiology in association with the lack of in vitro diagnostic methods for the certain and quick determination of the causative microbiological agents and their antibiotic resistance means the condition is still critical and of high impact in health care. The current gold standard method to detect the sepsis-causing pathogens, which is based on blood culture, is still insufficiently sensitive and slow. The new culture-independent molecular biology-based techniques can lead to the identification of a broad range of microorganisms and resistance markers within a few hours and with high sensitivity and specificity; nevertheless, limitations of, for example, the polymerase chain reaction-based methods still hamper their application in the clinical routine. This review summarizes the in vitro diagnostic methods and their approach in the clinical diagnosis of the bloodstream infections, and explores their advantages and disadvantages at the current state of the art. A quick analysis of the future prospective in multiplex technologies for microbiological diagnosis of sepsis is also provided.

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Multiplex Identification of Gram-Positive Bacteria and Resistance Determinants Directly from Positive Blood Culture Broths: Evaluation of an Automated Microarray-Based Nucleic Acid Test

Abstract: Nathan Ledeboer and colleagues assess the performance of a diagnostic platform for detecting Gram-positive bacteria in blood cultures, an important step in the diagnosis and treatment of patients with sepsis. Please see later in the article for the Editors' Summary

Cited by 113 publications

References 57 publications

Implementation of a Rapid Microarray Assay for the Detection of Gram-Positive, Gram-Negative and Resistance Determinants: Assessing Performance in a Clinical Laboratory in Bulgaria

Implementation of a Rapid Microarray Assay for the Detection of Gram-Positive, Gram-Negative and Resistance Determinants: Assessing Performance in a Clinical Laboratory in Bulgaria

Survey of Enteric Pathogens Causing Bacteremia in Cancer Patients

In vitro diagnosis of sepsis: a review

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