Multiplex DNA Biosensor for Viral Infection Diagnosis Using SERS Molecular Sentinel-on-Chip

Ngo, Hoan T.; Wang, Hsin‐Neng; Burke, Thomas W.; Woods, Christopher W.; Ginsburg, Geoffrey S.; Vo‐Dinh, Tuan

doi:10.1007/978-3-319-11776-8_4

Cited by 1 publication

(1 citation statement)

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“…In addition, immune-related genes were significantly changed after a 30-day exposure of ammonia nitrogen (Table 5). Among them, IFI44L is a kind of critical protein in host immune response to viral infections and can be used as molecular signature for viral infection diagnosis (Ngo et al, 2015). C-type lectin 9a belongs to the NK cell receptor group of C-type lectins and plays important roles in apoptosis and immune response against pathogens (Sancho et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short- and long-term exposures of ammonia nitrogen

Cong

Cao

et al. 2018

Aquatic Toxicology

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Previous study revealed severe toxic effects of ammonia nitrogen on Ruditapes philippinarum including lysosomal instability, disturbed metabolic profiles, gill tissues with damaged structure, and variation of neurotransmitter concentrations. However, the underlying molecular mechanism was not fully understood yet. In the present study, digital gene expression technology (DGE) was applied to globally screen the key genes and pathways involved in the responses to short- and long-term exposures of ammonia nitrogen. Results of DGE analysis indicated that short-term duration of ammonia exposure affected pathways in Dorso-ventral axis formation, Notch signaling, thyroid hormone signaling and protein processing in endoplasmic reticulum. The long-term exposure led to DEGs significantly enriched in gap junction, immunity, signal and hormone transduction, as well as key substance metabolism pathways. Functional research of significantly changed DEGs suggested that the immunity of R. philippinarum was weakened heavily by toxic effects of ammonia nitrogen, as well as neuro-transduction and metabolism of important substances. Taken together, the present study provides a molecular support for the previous results of the detrimental toxicity of ammonia exposure in R. philippinarum, further work will be performed to investigate the specific genes and their certain functions involved in ammonia toxicity to molluscs.

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Section: Discussionmentioning

confidence: 99%