Multiplex Detection of Fluorescent Chemokine Binding to CXC Chemokine Receptors by NanoBRET
Justyna M. Adamska,
Spyridon Leftheriotis,
Reggie Bosma
et al.
Abstract:NanoLuc-mediated bioluminescence resonance energy transfer (NanoBRET) has gained popularity for its ability to homogenously measure ligand binding to G protein-coupled receptors (GPCRs), including the subfamily of chemokine receptors. These receptors, such as ACKR3, CXCR4, CXCR3, play a crucial role in the regulation of the immune system, are associated with inflammatory diseases and cancer, and are seen as promising drug targets. The aim of this study was to optimize NanoBRET-based ligand binding to NLuc-ACKR… Show more
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