Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform

Pöhlmann, Christopher; Bellanger, Laurent; Drevinek, Michal; Elßner, Thomas

doi:10.1016/j.protcy.2017.04.045

Cited by 5 publications

(4 citation statements)

References 2 publications

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“…Most recently, portable BioDetector integrated (pBDi), a portable biothreat detection unit approximately the size of a small suitcase, has been developed to detect four Class A biothreats: Y. pestis, B. anthracis, F. tularensis, and orthopox viruses (making it one of the few electrochemical sensors for smallpox) 89 . Using gold electrodes conjugated with targeted antibodies, the system enables the specific binding of an antibody to its subsequent antigen.…”

Section: Combined Sensingmentioning

confidence: 99%

“…a pBDi system including reagents and sample holder with ruggedized tablet PC. b Principle of biothreat agent detection using electrochemical ELISA technology 89 from complex samples. This method simplifies the operation process and saves time.…”

Section: Future Of the Electrochemical Sensing Of Bioweaponsmentioning

confidence: 99%

“…The works by Pöhlmann et al 89 established a great foundation for the future of biosensing platforms. These platforms are extremely portable and detect four different Class A bioweapons.…”

Section: Future Of the Electrochemical Sensing Of Bioweaponsmentioning

confidence: 99%

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The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

2021

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During the past year, disease has shown us the iron grip it can hold over a population of people. Health systems can be overwhelmed, economies can be brought into recession, and many people can be harmed or killed. When weaponized, diseases can be manipulated to create a detriment to health while becoming an economic burden on any society. It is consequently prudent that easy detection of bioweapons is available to governments for protecting their people. Electrochemical sensing displays many distinct advantages, such as its low limit of detection, low cost to run, rapid generation of results, and in many instances portability. We therefore present a wide array of electrochemical sensing platforms currently being fabricated, a brief summary of Class A bioweapons, and the potential future of bioweapon detection and biosafety.

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Section: Combined Sensingmentioning

confidence: 99%

Section: Future Of the Electrochemical Sensing Of Bioweaponsmentioning

confidence: 99%

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The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

2021

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“…Quite early during development, the first applications based on this electrochemical biochip technology focused on multiplex detection of security sensitive toxins were developed using a prototype reader platform (ePaTox system) [ 142 , 143 ]. Based on these promising results, Bruker developed a portable and ruggedized platform for on-site usage called pBDi as well as several commercially available detection kits for the detection of security sensitive proteotoxins, bacteria and orthopox viruses [ 92 , 93 ]. The pBDi employs electrochemical biochip technology for multiplex fully automated ELISA-based detection of five to six agents simultaneously (for commercially available pBDi Test Kits; for customer specific assays in development, 12-plex immunoassays were already demonstrated).…”

Section: Portable Immunoassay Techniques With Multiplexing Capabilmentioning

confidence: 99%

Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Pöhlmann

Elßner

2020

Toxins

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Biological toxins are a heterogeneous group of high molecular as well as low molecular weight toxins produced by living organisms. Due to their physical and logistical properties, biological toxins are very attractive to terrorists for use in acts of bioterrorism. Therefore, among the group of biological toxins, several are categorized as security relevant, e.g., botulinum neurotoxins, staphylococcal enterotoxins, abrin, ricin or saxitoxin. Additionally, several security sensitive toxins also play a major role in natural food poisoning outbreaks. For a prompt response to a potential bioterrorist attack using biological toxins, first responders need reliable, easy-to-use and highly sensitive methodologies for on-site detection of the causative agent. Therefore, the aim of this review is to present on-site immunoassay platforms for multiplex detection of biological toxins. Furthermore, we introduce several commercially available detection technologies specialized for mobile or on-site identification of security sensitive toxins.

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Field-Based Multiplex Detection of Biothreat Agents

Pöhlmann

Elßner

2018

Enhancing CBRNE Safety &Amp; Security: Proceedings of the SICC 2017 Conference

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Multiplex Detection of Biothreat Agents Using an Automated Electrochemical ELISA Platform

Cited by 5 publications

References 2 publications

The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins

Field-Based Multiplex Detection of Biothreat Agents

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