Multiplex Asymmetric PCR by Combining the Amplification Refractory Mutation System with the Homo-Tag-Assisted Nondimer System
Ying Liu,
Yinghua Liu,
Liu Guo
et al.
Abstract:Asymmetric PCR is widely used to produce single-stranded
amplicons
(ss-amplicons) for various downstream applications. However, conventional
asymmetric PCR schemes are susceptible to events that affect primer
availability, which can be exacerbated by multiplex amplification.
In this study, a new multiplex asymmetric PCR approach that combines
the amplification refractory mutation system (ARMS) with the homo-Tag-assisted
nondimer system (HANDS) is described. ARMS-HANDS (A-H) PCR utilizes
equimolar-tailed forwar… Show more
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