Multiplex assay of allergen-specific and total immunoglobulins of E and G classes in the biochip format

Rubina, A. Yu.; Feizkhanova, G. U.; Filippova, M. A.; Talibov, V.; Fooke-Achterrath, M; Заседателев, А. С.

doi:10.1134/s1607672912060063

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…The protocol for the analysis and verification of this method were described in previous studies [ 14 , 15 , 16 ]. Briefly the procedure of the analysis on a microarray consisted of two steps: incubation with 65 μl of serum for 20 hours at 37°C and incubation with 50 μl of developing antibodies (solution of anti-IgE-Cy5 and/or anti-IgG4-Cy3 antibodies) for 1 hour at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

et al. 2018

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The immunological profiles of human specific IgE (sIgE) and specific IgG4 (sIgG4) vary by genetic predisposition, living conditions in different geographical locations and patient’s age. The aim of our study was to analyze sIgE and sIgG4 patterns and their age-dependent changes in patients from the Moscow region. For identifying sIgE and sIgG4 profiles the blood samples from 513 patients aged 6 months to 17 years who were showing symptoms of allergic diseases were analyzed using microarrays containing 31 allergens. The highest sIgE prevalence was observed for birch pollen (32%) among pollen allergens, cat dander (24%) among indoor allergens, and egg whites (21%) among food allergens. The most common sIgG4 response was developed toward egg whites (80% of patients). Age-related elevation was identified for patients with increased sIgE to pollen allergens and indoor allergens (cat or dog dander and house dust mites). For each allergen, the proportion of cases with significant levels of sIgG4 appeared to increase with patients’ age. The data on allergen-specific sIgE and sIgG4 prevalence show both general trends and some local special aspects that are indicative for the Moscow region. This information should be useful in terms of epidemiology of allergic diseases.

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Section: Methodsmentioning

confidence: 99%

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

et al. 2018

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“…The study conducted by Rubina et al in cooperation with Fooke-Achterrath [14] demonstrated the possibility of simultaneous sIgE and sIgG4 detection using hydrogel biochips. As a result of this approach, in this paper we have developed a method for the simultaneous quantitative analysis of allergen-specific immunoglobulins E and G4 to 31 allergens belonging to different groups.…”

Section: Introductionmentioning

confidence: 99%

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

et al. 2017

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BackgroundsIgE and sIgG4 detection is necessary for more accurate and effective type I hypersensitivity diagnostics and the estimation of disease development. Typically, the analyses of these antibodies are performed separately with the help of various specialized systems. The aim of this study was to develop a microarray-based method for the simultaneous quantitative detection of sIgE and sIgG4 to the most common allergens in a single sample.MethodsA quantitative method for the simultaneous detection of sIgE and sIgG4 was developed based on the technology of hydrogel microchips previously designed at Engelhardt Institute of Molecular Biology, Russian Academy of Sciences (EIMB RAS). The microarray contained gel pads with immobilized allergens and gel pads that allow for the obtaining of sIgE and sIgG4 internal calibration curves for each allergen during the assay. The possibility of the simultaneous detection of sIgE and sIgG4 was developed using the corresponding Cy5 and Cy3 fluorescent dyes.ResultsThe multiplex immunoassay method using hydrogel microarrays developed in this study allowed the quantitative detection of sIgE and sIgG4 to 31 allergens from different groups in a single assay. A comparison of the microarray with the existing plate-based analogues (i.e., ALLERG-O-LIQ and sIgG4 ELISA) was performed by analysing 152 blood serum samples and by evaluating Pearson correlation coefficients, ROC analysis, and Passing-Bablok linear regression results.ConclusionThe implementation of this method in allergy diagnostics will provide the possibility of simultaneously performing primary patient screening and obtaining additional information concerning the severity of the allergies and the choice of an appropriate therapy.

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Experience of Molecular Allergy Diagnosis in Kyrgyzstan

Turatbekova,

Sulaimanov,

Asheraliev

et al. 2024

Bulletin of Science and Practice

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Spectrum of allergenic sensitization in children of the Kyrgyz Republic using a modern molecular allergy test — ALEX. The study was conducted on 45 patients seeking medical help. Based on the results of the data obtained, we can conclude that molecular diagnostics make it possible to distinguish with high accuracy between true and cross-reactivity, which has significant clinical significance for adequate diagnosis of a wide range of sensitization to allergens and an informed choice of pathogenetic therapy.

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Multiplex assay of allergen-specific and total immunoglobulins of E and G classes in the biochip format

Cited by 3 publications

References 8 publications

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

Patterns of sensitization to inhalant and food allergens among pediatric patients from the Moscow region (Russian Federation)

Development of a microarray-based method for allergen-specific IgE and IgG4 detection

Experience of Molecular Allergy Diagnosis in Kyrgyzstan

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