Multiplex Analysis of Cytokines, Chemokines, Growth Factors, MMP-9 and TIMP-1 Produced by Human Bone Marrow, Adipose Tissue, and Placental Mesenchymal Stromal Cells

Ostanin, А. А.; Petrovskii, Ya. L.; Shevela, Е. Ya.; Черных, Е. Р.

doi:10.1007/s10517-011-1275-2

Cited by 28 publications

(27 citation statements)

References 29 publications

(22 reference statements)

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“…In our present study, we were able to reproduce our results with the 5 dose protocol of The mechanisms of action hypothesized for MSCs anti-inflammatory properties have been investigated in many studies [55][56][57][58][59][60]. aMSCs showed enhanced anti-inflammatory IL-10 secretion [73] together with decreased pro-inflammatory TNF-α, INF-γ, IL-1β and their downstream effects on various elements of the immune system.…”

Section: Discussionsupporting

confidence: 59%

“…CD11b and CD45) [74][75][76]. In addition to their multilineage differentiation potential, aMSCs have anti-inflammatory/immune-modulatory and paracrine effects [55][56][57][58][59][60]. They also have the ability to home to the site of tissue injury after irradiation and inflammation [74,77,78].…”

Section: Methodsmentioning

confidence: 99%

“…MSCs are multipotent progenitor cells that have multi-lineage differentiation potential and recently identified anti-inflammatory and immunomodulatory properties that have been applied in numerous current preclinical and clinical studies [55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70].…”

Section: Mesenchymal Stromal/stem Cells Therapymentioning

confidence: 99%

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Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

et al. 2016

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Section: Discussionsupporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%

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Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

et al. 2016

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“…Although a few studies attempted to unravel the functional differences among AT-, BM- and UCM-MSCs [72,121,122], recent works shed light on this subject, showing that AT-MSCs express an higher level of COX-1 and PGE2 [71] and UCM-MSCs display a lower expression of PGE2 and IDO compared to BM-MSCs [67]. …”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

Ribeiro¹,

Laranjeira²,

Mendes³

et al. 2013

Stem Cell Res Ther

223

184

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IntroductionThe ability to self-renew, be easily expanded in vitro and differentiate into different mesenchymal tissues, render mesenchymal stem cells (MSCs) an attractive therapeutic method for degenerative diseases. The subsequent discovery of their immunosuppressive ability encouraged clinical trials in graft-versus-host disease and auto-immune diseases. Despite sharing several immunophenotypic characteristics and functional capabilities, the differences between MSCs arising from different tissues are still unclear and the published data are conflicting.MethodsHere, we evaluate the influence of human MSCs derived from umbilical cord matrix (UCM), bone marrow (BM) and adipose tissue (AT), co-cultured with phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (MNC), on T, B and natural killer (NK) cell activation; T and B cells’ ability to acquire lymphoblast characteristics; mRNA expression of interleukin-2 (IL-2), forkhead box P3 (FoxP3), T-bet and GATA binding protein 3 (GATA3), on purified T cells, and tumor necrosis factor-alpha (TNF-α), perforin and granzyme B on purified NK cells.ResultsMSCs derived from all three tissues were able to prevent CD4+ and CD8+ T cell activation and acquisition of lymphoblast characteristics and CD56dim NK cell activation, wherein AT-MSCs showed a stronger inhibitory effect. Moreover, AT-MSCs blocked the T cell activation process in an earlier phase than BM- or UCM-MSCs, yielding a greater proportion of T cells in the non-activated state. Concerning B cells and CD56bright NK cells, UCM-MSCs did not influence either their activation kinetics or PHA-induced lymphoblast characteristics, conversely to BM- and AT-MSCs which displayed an inhibitory effect. Besides, when co-cultured with PHA-stimulated MNC, MSCs seem to promote Treg and Th1 polarization, estimated by the increased expression of FoxP3 and T-bet mRNA within purified activated T cells, and to reduce TNF-α and perforin production by activated NK cells.ConclusionsOverall, UCM-, BM- and AT-derived MSCs hamper T cell, B cell and NK cell-mediated immune response by preventing their acquisition of lymphoblast characteristics, activation and changing the expression profile of proteins with an important role in immune function, except UCM-MSCs showed no inhibitory effect on B cells under these experimental conditions. Despite the similarities between the three types of MSCs evaluated, we detect important differences that should be taken into account when choosing the MSC source for research or therapeutic purposes.

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“…Several modulators of the survival of CLL cells have been identified and include soluble factors and cell-cell interactions [9], [10]. Nurse-like cells [11], bone marrow stromal cells [12], [13], T cells [14], [15], dendritic cells [16], accessory leukocytes [17], the leukemic cells themselves [18] and the cytokines derived from them: BAFF and APRIL [19], IL-6 [20], IL-10 [21], IL-2 [22], IL-4 [23], IL-21 [24], IL-12 [25] and IL-15 [26], [27], have all been suggested to act as pro-survival factors or growth factors in CLL. Nevertheless, it is unclear whether all the cytokines that promote viability in vitro also play a significant role in vivo ; it is consequently relevant to study the effect of humoral components on CLL B-cell survival [28], [29].…”

Section: Introductionmentioning

confidence: 99%

A Combination of Cytokines Rescues Highly Purified Leukemic CLL B-Cells from Spontaneous Apoptosis In Vitro

et al. 2013

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B-chronic lymphocytic leukemia (B-CLL), the most common human leukemia, is characterized by predominantly non-dividing malignant mature CD5+ B lymphocytes with an apoptosis defect. Various microenvironmental stimuli confer a growth advantage on these leukemic cells and extend their survival in vivo. Nevertheless, when cultured in vitro, CLL B-cells rapidly die from apoptosis. Certain cytokines may extend the survival capacity of CLL B-cells in vitro and individual anti-apoptotic effects of several cytokines have been reported. The potential cumulative effect of such cytokines has not been studied. We therefore investigated the effects on CLL B-cells survival in vitro of humoral factors, polyclonal lymphocyte activators and a combination of cytokines known for their anti-apoptotic effects. Purified CLL B-cells were cultured in the presence or absence of various soluble molecules and the leukemic cell response was assessed in terms of viability. Apoptotic cell death was detected by flow cytometry using annexinV and 7-amino-actinomycin. The survival of CLL B-cells in vitro was highly variable. When tested separately, cytokines (IL-2, -6, -10, -12, -15, -21, BAFF and APRIL) improved CLL B cell survival moderately; in combination, they significantly enhanced survival of these cells, even up to 7 days of culture. We also report that humoral factors from autologous serum are important for survival of these malignant cells. Our findings support the concept that the CLL microenvironment is critical and suggest that soluble factors may contribute directly to the prolonged survival of CLL B-cells. Therefore, the combination of cytokines we describe as providing strong resistance to apoptosis in vitro might be used to improve the treatment of CLL.

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Multiplex Analysis of Cytokines, Chemokines, Growth Factors, MMP-9 and TIMP-1 Produced by Human Bone Marrow, Adipose Tissue, and Placental Mesenchymal Stromal Cells

Cited by 28 publications

References 29 publications

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis

Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells

A Combination of Cytokines Rescues Highly Purified Leukemic CLL B-Cells from Spontaneous Apoptosis In Vitro

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