2005
DOI: 10.1002/elps.200406129
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Multiplex amplified product-length polymorphism analysis of 36 mitochondrial single-nucleotide polymorphisms for haplogrouping of East Asian populations

Abstract: We present a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the haplogroup-diagnostic mitochondrial single-nucleotide polymorphisms (mtSNPs) in East Asian populations. By examining only 36 haplogroup-specific mtSNPs in the coding region by using four 9-multiplex polymerase chain reaction (PCR) and subsequent electrophoresis, we could safely assign 1815 individuals from 8 populations of Japanese, Korean, Chinese, and Germans to 45 relevant haplogroups… Show more

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Cited by 75 publications
(88 citation statements)
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“…However, the basic concept of the 'dual' structure model for Japanese population history (Hanihara, 1991) or of the 'double' strata (Yamaguchi, 1986) has been sufficiently valid in other regions of the Japanese archipelago, and remains so (Saitou, 2005), because Yamaguchi (1986) and Hanihara (1991) presented the 'double migration' story as the simplest model for the population history of Japan. Moreover, there may be no sense in arguing for and against the 'Ainu-Ryukyu common origin theory', because the modern peoples in the Japanese archipelago, including the modern Japanese, should more or less have traces of the Jomon, as indicated by mtDNA analysis (Umetsu et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…However, the basic concept of the 'dual' structure model for Japanese population history (Hanihara, 1991) or of the 'double' strata (Yamaguchi, 1986) has been sufficiently valid in other regions of the Japanese archipelago, and remains so (Saitou, 2005), because Yamaguchi (1986) and Hanihara (1991) presented the 'double migration' story as the simplest model for the population history of Japan. Moreover, there may be no sense in arguing for and against the 'Ainu-Ryukyu common origin theory', because the modern peoples in the Japanese archipelago, including the modern Japanese, should more or less have traces of the Jomon, as indicated by mtDNA analysis (Umetsu et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, M7a is one of the prevailing haplogroups not only among modern Japanese, including Honshu, Okinawa islanders, and Ainu populations (Tanaka et al 2004), but also in the Jomon population (Adachi et al 2011). The frequency of haplogroup M7a among modern Japanese is highest in the Okinawa islanders (23.3 per cent; Umetsu et al 2005) -gradually decreasing towards the northern part of Honshu (Shinoda 2007). This finding indicates that this haplogroup may have a southern origin.…”
Section: Resultsmentioning
confidence: 99%
“…To confidently assign mtDNA samples to relevant haplogroups, 24 haplogroup-diagnostic single nucleotide polymorphisms (SNPs), including a 9 bp repeat variation in the non-coding cytochrome oxidase II/tRNALys intergenic region, that defines major haplogroups found in Japanese and East Asian populations, were analysed by multiplex APLP (Umetsu et al 2005). SNPs that defined major haplogroups were detected by using suspension array technology (Luminex 100) at the laboratory of G&G Science, Fukushima.…”
Section: Terra Australis 45mentioning
confidence: 99%
“…Moreover, M7a is one of the prevailing haplogroups not only among modern Japanese, including Honshu, Okinawa islanders, and Ainu populations (Tanaka et al 2004), but also in the Jomon population (Adachi et al 2011). The frequency of haplogroup M7a among modern Japanese is highest in the Okinawa islanders (23.3 per cent; Umetsu et al 2005) -gradually decreasing towards the northern part of Honshu (Shinoda 2007). This finding indicates that this haplogroup may have a southern origin.…”
Section: Resultsmentioning
confidence: 99%
“…Sequencing was performed in both directions to enable identification of polymorphisms or ambiguous bases by using a single primer. The sequencing reactions were performed on a DNA Sequencer (ABI model 3130) equipped with SeqEd software (ABI).To confidently assign mtDNA samples to relevant haplogroups, 24 haplogroup-diagnostic single nucleotide polymorphisms (SNPs), including a 9 bp repeat variation in the non-coding cytochrome oxidase II/tRNALys intergenic region, that defines major haplogroups found in Japanese and East Asian populations, were analysed by multiplex APLP (Umetsu et al 2005). SNPs that defined major haplogroups were detected by using suspension array technology (Luminex 100) at the laboratory of G&G Science, Fukushima.…”
mentioning
confidence: 99%