Multiple α-galactosidases from Aspergillus niger: purification, characterization and substrate specificities

Ademark, Pia; Larsson, Martin; Tjerneld, Folke; Stålbrand, Henrik

doi:10.1016/s0141-0229(01)00415-x

Cited by 80 publications

(67 citation statements)

References 25 publications

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“…in submerged 120-144 h shaking cultures, this strain produced about 27-28% lower enzyme yield (5450-5500U/l). It is evident from our results that A. niger M 47 is also a rich source of α-galactosidase activity, which is several-fold higher than the values reported by other workers for A. niger strains (1,9,26). There is little information about α-galactosidase excreted by fungi grown on soya-based substrates.…”

Section: Shake-flask Submerged and Stationary Cultivationcontrasting

confidence: 72%

“…5) the biomass concentration was reduced almost twice from its maximal value of 12.1 g/l in the 48 th h to 5.6 g/l in the 120 th h. Maximum α-galactosidase activity occurred at ph 4.8, also in the absence of ph control. the best enzyme yields of α-galactosidase by P. chrysogenum ln 33 and A. niger M 47 were several-fold higher than the activities reported for other fungi belonging to the genera Aspergillus, Penicillium, Thermomyces, Humicola and Trichoderma (1,4,7,8,9,11,16,19,26,30).…”

Section: Bioreactor Performancementioning

confidence: 78%

“…The α-galactosidases (α-D-galactoside galactohydrolase, EC 3.2.1.22) are carbohydrases which catalyse hydrolysis of α-1,6-linked α-galactoside residual from simple oligosaccharides such as melibiose, raffinose, stachyose and from polymeric galactomannans (1). these enzymes are used in the hydrolysis of raffinose and stachyose present in soy beans and other leguminous food and feed that cause intestinal discomfort, flatulence and low feed utilization in monogastrites (12).…”

Section: Introductionmentioning

confidence: 99%

“…Abundant information is available on the biosynthesis of α-galactosidase from filamentous fungi belonging to the genera Penicillium and Aspergillus (1,4,6,8,9,16,18,21,23,25,26) during submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

High-Yield Production of Alpha-Galactosidase Excreted fromPenicillium ChrysogenumandAspergillus Niger

Алексиева

Tchorbanov

Nacheva

2010

Biotechnology & Biotechnological Equipment

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Section: Shake-flask Submerged and Stationary Cultivationcontrasting

confidence: 72%

Section: Bioreactor Performancementioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High-Yield Production of Alpha-Galactosidase Excreted fromPenicillium ChrysogenumandAspergillus Niger

Алексиева

Tchorbanov

Nacheva

2010

Biotechnology & Biotechnological Equipment

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“…this enzyme is used in food processing, the sugar industry, medicine, enzymatic synthesis and structural analysis (13,15). Most investigated α-galactosidases are produced by free cells of filamentous fungi, belonging to the genera Aspergillus, Penicillium and Humicola (1,12,14). the immobilization of the enzyme, that can be applied for removal of flatulenceinducing sugars in soymilk was reported (16).…”

Section: Introductionmentioning

confidence: 99%

Sol-Gel Nanomaterials with Algal Heteropolysaccharide for Immobilization of Microbial Cells, Producing A-Galactosidase and Nitrilase

Djambaski¹,

Алексиева²,

Chernev³

et al. 2009

Biotechnology & Biotechnological Equipment

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Biofuels: Fungal, Bacterial and Insect Degraders of Lignocellulose

Sethi

Scharf

2013

Encyclopedia of Life Sciences

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Lignocellulose is the main structural component of plant cell walls and can be degraded into simple sugars with the help of hydrolytic enzymes. Lignocellulose is composed of three polymers: cellulose, hemicellulose and lignin. Certain fungi, bacteria and insects have evolved the ability to degrade lignocellulose. Cellulose is degraded to glucose by the synergistic action of three distinct classes of enzymes: endoglucanases, exoglucanases and β‐glucosidases. Owing to its variable structure and organisation, hemicellulose degradation requires various enzymes with diverse modes of action, which include endoxylanases, endomannanases, β‐xylosidases, β‐mannosidases, β‐galactosidases, α‐glucuronidases, α‐arabinofurnosidases, α‐galactosidases, acetyl xylan esterases, feruloyl esterases and glucuronyl esterases. Lignin degradation is much more difficult due to its complex structure and bonding to carbohydrate complexes. It requires oxidative enzymes, such as lignin peroxidases, manganese peroxidases, versatile peroxidases, laccases, and several other auxiliary enzymes. Commercial biofuel production from lignocellulosic materials can be achieved by artificially producing these cellulases, hemicellulases and lignases in recombinant form. Key Concepts: Lignocellulose is the most abundant renewable resource of energy on the Earth. Lignocellulose is a general term referring to a natural complex of three biopolymers: cellulose, hemicellulose and lignin. Simple sugar can be released from lignocellulose using three categories of enzymes: cellulases, hemicellulase and lignases. Released sugars from lignocellulose can be fermented into biofuel (ethanol). Wood‐rotting fungi such as white‐rot, brown‐rot and soft‐rot fungi can degrade lignocellulose. Aerobic and anaerobic bacteria such as Bacillus, Streptomyces, Cellulomonas and Clostridium possess lignocellulose‐degrading enzymes. Wood and plant‐feeding insects such termites, roaches, longhorn beetles and grasshoppers are rich sources of novel lignocellulases genes. Lignocellulases of fungal, bacterial and insect origin can be artificially/recombinantly produced in various protein expression systems. Fungal, bacterial and insect lignocellulases are expected to play important roles in the development of successful lignocellulose conversion technologies.

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Multiple α-galactosidases from Aspergillus niger: purification, characterization and substrate specificities

Cited by 80 publications

References 25 publications

High-Yield Production of Alpha-Galactosidase Excreted fromPenicillium ChrysogenumandAspergillus Niger

High-Yield Production of Alpha-Galactosidase Excreted fromPenicillium ChrysogenumandAspergillus Niger

Sol-Gel Nanomaterials with Algal Heteropolysaccharide for Immobilization of Microbial Cells, Producing A-Galactosidase and Nitrilase

Biofuels: Fungal, Bacterial and Insect Degraders of Lignocellulose

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