Multiple Viral Infections and Genomic Divergence among Noroviruses during an Outbreak of Acute Gastroenteritis

Sasaki, Yukiko; Kai, Akemi; Hayashi, Yujiro; Shinkai, Takayuki; Noguchi, Yayoi; Hasegawa, Minoru; Sadamasu, Kenji; Mori, Kohji; Tabei, Yutaka; Nagashima, Mami; Morozumi, Satoshi; Yamamoto, Tomoko

doi:10.1128/jcm.44.3.790-797.2006

Cited by 21 publications

(10 citation statements)

References 34 publications

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“…Acute gastroenteritis in humans has been associated with a number of different viruses. Of these, rotaviruses (RV), noroviruses (NoV), sapoviruses (SaVs), human astroviruses (HAstVs), and enteric adenoviruses (EAds) are the most common causes of sporadic gastroenteritis requiring hospitalization, and together constitute a major burden for public health systems worldwide [Glass et al, 2000[Glass et al, , 2006Sasaki et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

“…These environments include hospitals, day care centers, nursing homes, hotels, cruise ships, and schools [Borges et al, 2006;Bull et al, 2006;Sasaki et al, 2006]. Since the 1990s, the use of molecular methods has also highlighted the role of NoV as a common agent of sporadic, self-limiting episodes of acute gastroenteritis, which are normally caused by the consumption of contaminated food or water [Bull et al, 2006].…”

Section: Introductionmentioning

confidence: 99%

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Norovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazil

Siqueira¹,

Linhares²,

Carvalho³

et al. 2013

Journal of Medical Virology

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Noroviruses are the leading cause of epidemic, non-bacterial outbreaks of acute gastroenteritis, and are also a major cause of sporadic acute gastroenteritis in infants. The aim of the present study was to identify norovirus infections in children not infected by rotavirus admitted to hospital for acute gastroenteritis in Belém. A total of 348 fecal specimens were obtained from children with diarrhea aged less than 5 years, all of whom had tested negative for rotavirus, between May 2008 and April 2010. Fecal samples were screened for norovirus antigen using enzyme-immunoassay (EIA). Specimens were subjected to reverse-transcription polymerase chain reaction (RT-PCR) using the primers Mon432/434-Mon431/433 for detection of the GI and GII norovirus strains, respectively. Based on both methods, the overall norovirus positivity rate was 36.5% (127/348). Of the 169 samples collected in the first year, 44.4% (n = 75) tested positive for norovirus using both methods, 35.5% (n = 60) by EIA and 40.8% (n = 69) by RT-PCR. Using RT-PCR as a reference standard, a sensitivity of 78.3%, specificity of 94%, and agreement of 87.6% were recorded. Genome sequencing was obtained for 22 (31.9%) of the 69 positive samples, of which 90.9% (20/22) were genotype GII.4d and 9.1% (2/22) were genotype GII.b. Norovirus infection was most frequent in children under 2 years of age (41.5%-115/277). The peak incidence (62.1%) of norovirus-related acute gastroenteritis in these patients (not infected by rotavirus) was observed in February 2010. These findings emphasize the importance of norovirus as a cause of severe acute gastroenteritis among children in Belém, Pará, Northern Brazil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Norovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazil

Siqueira¹,

Linhares²,

Carvalho³

et al. 2013

Journal of Medical Virology

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“…Both ordinary and real time PCR products were also sequenced in cases of very low Ct values or detection by only real time PCR. Sequencing was performed as previously described [12].…”

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confidence: 99%

Application of Real Time PCR for Diagnosis of Swine Dysentery

Akase

Uchitani

Sohmura

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.

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“…NoVs are now recognized as the leading cause of epidemic viral gastroenteritis, and the genogroups GI, GII, and occasionally GIV are associated with human infection (17,22,24). HAstV and EAds are also recognized as important viral agents of diarrhea in infants and the elderly (31). Although the association between HBoV2 and gastroenteritis is still unknown, the virus has been frequently found in fecal samples from children with gastroenteritis (3,8,18).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Detection of Seven Enteric Viruses Associated with Acute Gastroenteritis by a Multiplexed Luminex-Based Assay

Liu

Zhang

et al. 2012

J Clin Microbiol

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Rapid and broad diagnostic methods are needed for the identification of viral agents of gastroenteritis. In this study, we used Luminex xMAP technology to develop a multiplexed assay for the simultaneous identification of major enteric viral pathogens, including rotavirus A (RVA), noroviruses (NoVs) (including genogroups GI and GII), sapoviruses (SaV), human astrovirus (HAstV), enteric adenoviruses (EAds), and human bocavirus 2 (HBoV2). The analytical sensitivity allowed detection of 10 3 (EAds, HBoV2, and RVA) and 10 4 (NoV GI and GII, SaV, and HAstV) copies per reaction mixture. Compared to conventional PCR, the Luminex-based assay yielded greater than 75% sensitivity and 97% specificity for each virus, and the kappa correlation for detection of all viruses ranged from 0.75 to 1.00. In conclusion, this multiplexed Luminex-based assay provides a potentially rapid, high-throughput, and maneuverable diagnostic tool for major viral pathogens associated with gastroenteritis.

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Multiple Viral Infections and Genomic Divergence among Noroviruses during an Outbreak of Acute Gastroenteritis

Cited by 21 publications

References 34 publications

Norovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazil

Norovirus infection in children admitted to hospital for acute gastroenteritis in Belém, Pará, Northern Brazil

Application of Real Time PCR for Diagnosis of Swine Dysentery

Simultaneous Detection of Seven Enteric Viruses Associated with Acute Gastroenteritis by a Multiplexed Luminex-Based Assay

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