Multiple VH gene segments encode murine antistreptococcal antibodies.

Perlmutter, Roger M.; Klotz, Joan L.; Bond, Michael D.; Nahm, Moon H.; Davie, Joseph M.; Hood, Leroy

doi:10.1084/jem.159.1.179

Cited by 48 publications

(14 citation statements)

References 43 publications

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“…These hybridomas have been used to study the V genes encoding the anti-GAC antibodies [38]. These studies have shown that anti-GAC antibodies are encoded by the restricted V genes, VHJ606 and V,24.…”

Section: Antibodies Using Vnj606 Gene Family Fl2-1fructosan (Inulin) mentioning

confidence: 99%

Molecular characteristics of anti-polysaccharide antibodies

Bona

1993

Springer Semin Immunopathol

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Section: Antibodies Using Vnj606 Gene Family Fl2-1fructosan (Inulin) mentioning

confidence: 99%

Molecular characteristics of anti-polysaccharide antibodies

Bona

1993

Springer Semin Immunopathol

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“…Furthermore, the Ick gene is specifically implicated in the pathogenesis of lymphoid malignancy by virtue of its rearrangement and overexpression in two independently obtained murine lymphoma cell lines, LSTRA and Thy-19 (6,18,37), and by its localization at a site of frequent chromosomal abnormalities (lp32-35) in human non-Hodgkin lymphomas (15). The gene is also overexpressed in some human colon and lung tumors (34 (22). A library of LSTRA DNA was constructed by MboI partial digestion followed by fractionation of 15-to 20-kilobase (kb) fragments on sucrose density gradients (14), ligation into EMBL3 bacteriophage arms, and packaging by standard techniques (11).…”

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confidence: 99%

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Garvin¹,

Pawar²,

Marth³

et al. 1988

Mol. Cell. Biol.

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The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in neoplastic transformation. We have determined the germ line organization of the murine lck gene and have isolated and characterized a rearranged lck allele in the murine lymphoma cell line LSTRA. The overall exon-intron organization of the normal lck gene is almost identical to that of avian c-src. In LSTRA DNA, an internally rearranged Moloney murine leukemia virus genome is interposed between two distinct promoters that normally generate lck transcripts differing only in 5' untranslated regions. The rearrangement appears to have been selected to permit splicing of transcripts that initiate from the Moloney virus promoter to an acceptor site located within the first exon 3' to the downstream promoter, thus generating an lck mRNA with a novel 5' untranslated region that may be more efficiently translated.

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“…The anti-␣(133) dextran Ab, for example, expresses mainly the V H J558 family (68); whereas anti-␤(231) fructosan Ab predominantly expresses the V H J606 gene family (11) and Abs to ␤(236) fructosan and ␤(136) galactan express the genes of the V H X24 family (42,67). The anti-group A streptococcal carbohydrate Ab reflects a germline repertoire that includes at least two V H gene families, one of which belongs to the V H J606 family paired with several V gene families (46). The response to the glucuronoxylomannan component of the capsular PS of Cryptococcus neoformans serogroup D uses the V H X24 family (13), and immunized mice respond with V H 7183 Ab specific for serogroup A (40), indicating the highly restricted usage of V H gene families in anti-PS responses and differences depending on the structure.…”

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confidence: 99%

Murine Immune Response toNeisseria meningitidisGroup C Capsular Polysaccharide: Analysis of Monoclonal Antibodies Generated in Response to a Thymus-Independent Antigen and a Thymus-Dependent Toxoid Conjugate Vaccine

et al. 2000

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Antibody (Ab) responses to polysaccharides (PSs) such as Neisseria meningitidis group C PS (MCPS) are characterized as being thymus independent (TI) and are restricted with regard to clonotype and isotype expression. PS conjugated to proteins, e.g., MCPS coupled to tetanus toxoid (MCPS-TT), elicits a thymusdependent (TD) response. In order to understand the influence of the form of a vaccine (TI versus TD) on the Ab repertoire, we generated monoclonal antibody (MAb) panels from mice immunized and boosted with MCPS or MCPS-TT in different ways. The panels of MAbs were examined for isotype, fine specificity, affinity, and V H gene family usage. The use of MCPS-TT resulted in a shift in the isotype from immunoglobulin M (IgM) and IgG3 elicited in response to the MCPS to primarily IgG1. This isotype shift was accompanied by a change in the fine specificity of the response to the conjugate compared to that of PS. New fine specificities and increased affinity were observed in response to the TD antigen (Ag). Dot blot and Northern analyses of MCPS MAbs revealed that V H gene family usage is dominated by V H J558, used by 23 of 39 MAbs. V H 3609 was seen in three MAbs of restricted fine specificity. V H Q52, V H 7183, and V H VGAM3-8 were seen in more than one MAb across these panels, while V H 10 and V H X24 were detected only once in response to the TI-2 Ag. All MAbs in the panels utilized kappa light chains, and all functional J genes were expressed.The capsular polysaccharide (PS) constitutes the major virulence factor of many pathogenic bacteria that cause invasive diseases. Antibodies (Abs) against these PSs are protective (27,28). PSs are classified as thymus-independent-2 (TI-2) antigens (Ags) because they do not require mature T cells to elicit a humoral response in vivo. These PS Ags are immunogenic in adults but are only poorly or nonimmunogenic in infants and young children who are highly susceptible to infection caused by encapsulated bacteria (28,31,44,65).The response to capsular PS is markedly different from the response to most protein Ags (thymus-dependent [TD] Ags). The Ab response to TI-2 Ag develops late in ontogeny (25,44,50) and in mice utilizes a particular late-developing subset of B cells that is defined by the expression of Lyb5 and other cell markers (39, 60). TI Ags also generally fail to elicit a memory response or show affinity maturation. In contrast, the ability to respond to a TD Ag is present at birth and results in the formation of memory cells, and the Ab response undergoes subsequent affinity maturation upon reimmunization (61). For TI Ag, immunoglobulin G3 (IgG3) and IgM are the major isotypes expressed in mice, even after secondary immunization (45), whereas for TD Ag, the ratio of IgG to IgM increases after secondary immunization, with IgG1 being the major subclass (52,59,60).The majority of the anti-PS responses are oligoclonal and encoded by a few variable regions of the heavy chain (V H ) gene families (10). The anti-␣(133) dextran Ab, for example, expresses mainly the V H J55...

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Multiple VH gene segments encode murine antistreptococcal antibodies.

Cited by 48 publications

References 43 publications

Molecular characteristics of anti-polysaccharide antibodies

Molecular characteristics of anti-polysaccharide antibodies

Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.

Murine Immune Response toNeisseria meningitidisGroup C Capsular Polysaccharide: Analysis of Monoclonal Antibodies Generated in Response to a Thymus-Independent Antigen and a Thymus-Dependent Toxoid Conjugate Vaccine

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