Multiple variants of phoP/Q and pmrAB TCRS conferring colistin resistance in XDR/MDR clinical isolates of Klebsiella pneumoniae in India

Yadav, Gulshan; Azam, Mudsser; Upmanyu, Kirti; Jain, Manisha; Gaind, Rajni; Singh, Ruchi

doi:10.1016/j.ijid.2020.09.076

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pneumoniae HS11286 [23] A change in protein level was explored with the NCBI BLASTx tool. To establish individual gene polymorphism for Colistin resistance, the phenotypic effect of amino acid substitutions on protein function were predicted using polymorphism phenotyping v2 web tool (PolyPhen-2) as described G. Yadav et al and Azam et al [24,25]. To determine the phylogenetic relatedness a concatenated marker gene maximum-likelihood tree was constructed using a number of K. pneumoniae reference genomes chosen based on BLAST similarity results, clonal complexes and sequence types.…”

Section: Gene Detection Multi-locus Sequence Typing (Mlst) and Phylog...mentioning

confidence: 99%

Whole Genome Sequencing Uncovers the Genetic Determinants of Colistin Resistance in Multi-Drug Resistant Klebsiella pneumoniae Isolated from Patients Admitted in the ICU of a Tertiary Care Hospital in Kampala, Uganda

Magulye,

Maghembe,

Sekyanzi

et al. 2023

Preprint

View full text Add to dashboard Cite

Multidrug resistance (MDR) among gram-negative bacteria is a global public health threat because it has exhausted all standard therapeutic options. This has necessitated clinicians to reconsider colistin, an “old sort” antibiotic which was deemed toxic for clinical use. Unfortunately, swift resistance towards colistin by K. pneumoniae has been reported in different parts of the world. The study aimed at investigating the genetic determinants of colistin resistance in K. pneumoniae isolated from patients admitted in a tertiary care hospital in Kampala, Uganda. 31 archived multidrug resistant K. pneumoniae isolates obtained from rectal swabs of patients admitted to the Mulago Hospital ICU were retrieved. From this collection, we selected 7 colistin resistant K. pneumoniae isolates for whole genome sequencing and molecular profile analysis. In silico sequence data analyses were performed using established web-based bioinformatics tools. The sequence analysis of the genes involved in colistin resistance revealed several non-synonymous mutations in nucleotide sequences of pmrA, pmrB, phoP, phoQ and mgrB genes. Insertion transposase genes for the insertion sequences IS1 and IS5 which are highly implicated in colistin resistance via modification or inactivation of mgrB gene were identified in all isolates and were located in the chromosomal DNA at different genomic positions. No plasmid-borne mcr genes were detected. More so, the strains harbored numerous multidrug resistant genes. This study underscores the need of genomic surveillance of K. pneumoniae strains to limit further spread of colistin resistance genes to avert the potential risk of pandrug resistance. And high throughput sequencing is imperative.

show abstract

Section: Gene Detection Multi-locus Sequence Typing (Mlst) and Phylog...mentioning

confidence: 99%

Whole Genome Sequencing Uncovers the Genetic Determinants of Colistin Resistance in Multi-Drug Resistant Klebsiella pneumoniae Isolated from Patients Admitted in the ICU of a Tertiary Care Hospital in Kampala, Uganda

Magulye,

Maghembe,

Sekyanzi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Multiple variants of phoP/Q and pmrAB TCRS conferring colistin resistance in XDR/MDR clinical isolates of Klebsiella pneumoniae in India

Cited by 1 publication

References 0 publications

Whole Genome Sequencing Uncovers the Genetic Determinants of Colistin Resistance in Multi-Drug Resistant Klebsiella pneumoniae Isolated from Patients Admitted in the ICU of a Tertiary Care Hospital in Kampala, Uganda

Whole Genome Sequencing Uncovers the Genetic Determinants of Colistin Resistance in Multi-Drug Resistant Klebsiella pneumoniae Isolated from Patients Admitted in the ICU of a Tertiary Care Hospital in Kampala, Uganda

Contact Info

Product

Resources

About