Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates

Guo, Yingshu; Liu, Jia; Yang, Guangxu; Sun, Xiaofeng; Chen, Hong‐Yuan; Xu, Jing‐Juan

doi:10.1039/c4cc08428d

Cited by 21 publications

(10 citation statements)

References 17 publications

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“…Recently lots of research work on hairpin DNA probe based immunosensors for the ultrasensitive detection of biomarkers has been reported. (Ge et al 2016;Gong et al 2014;Guo et al 2015;Yao et al 2014;Zhang et al 2014a;Zhang et al 2014b) This topic has been symmetrically reviewed. (Huang et al 2015b) As an example, a hairpin aptamer DNAzyme probe was used for sensitive and visual detection of IFN-γ based on an original quadratic amplification strategy ( Figure 5).…”

Section: Hairpin Dna Probes Based Amplification Strategymentioning

confidence: 99%

Recent advances in cytokine detection by immunosensing

Liu

Meng

Hutchinson

et al. 2016

Biosensors and Bioelectronics

115

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The detection of cytokines in body fluids, cells, tissues and organisms continues to attract considerable attention due to the importance of these key cell signaling molecules in biology and medicine. In this review, we describe recent advances in cytokine detection in the course of ongoing pursuit of new analytical approaches for these trace analytes with specific focus on immunosensing. We discuss recent elegant designs of sensing interface with improved performance with respect to sensitivity, selectivity, stability, simplicity, and the absence of sample matrix effects. Various immunosensing approaches based on multifunctional nanomaterials open novel opportunities for ultrasensitive detection of cytokines in body fluids in vitro and in vivo. Methodologies such as suspension arrays also known as bead assays together with optical fiber-based sensors, on their own or in combination with microfluidic devices will continue to have an important role to address the grand challenge of real-time in vivo multiplex cytokine detection.

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Section: Hairpin Dna Probes Based Amplification Strategymentioning

confidence: 99%

Recent advances in cytokine detection by immunosensing

Liu

Meng

Hutchinson

et al. 2016

Biosensors and Bioelectronics

115

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“…The released aptamer initiates a round of RCA, thus producing a detection limit as low as 60 nM. In addition, the binding of ATP to an inactivated DNAzyme containing ATP aptamer 57 or an ATP aptamer partially hybridized with a DNAzyme 58 can trigger the release of the active DNAzyme and the released DNAzyme can be engaged in the configuration of amplified detection of ATP.…”

Section: Fluorescent Aptasensorsmentioning

confidence: 99%

Optical Aptasensors for Adenosine Triphosphate

Ng¹,

Lim²,

Ma³

et al. 2016

Theranostics

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Nucleic acids are among the most researched and applied biomolecules. Their diverse two- and three-dimensional structures in conjunction with their robust chemistry and ease of manipulation provide a rare opportunity for sensor applications. Moreover, their high biocompatibility has seen them being used in the construction of in vivo assays. Various nucleic acid-based devices have been extensively studied as either the principal element in discrete molecule-like sensors or as the main component in the fabrication of sensing devices. The use of aptamers in sensors - aptasensors, in particular, has led to improvements in sensitivity, selectivity, and multiplexing capacity for a wide verity of analytes like proteins, nucleic acids, as well as small biomolecules such as glucose and adenosine triphosphate (ATP). This article reviews the progress in the use of aptamers as the principal component in sensors for optical detection of ATP with an emphasis on sensing mechanism, performance, and applications with some discussion on challenges and perspectives.

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“…The excess amount of ATP consumed by creatine kinase causes particular diseases. 3,4 Therefore, the real-time monitoring of ATP levels is imperative for the study of multiple cellular mechanisms, and enzyme activity involving the production and consumption of ATP as well as clinical diagnosis. 5,6 …”

Section: Introductionmentioning

confidence: 99%

Proximity hybridization triggered strand displacement and DNAzyme assisted strand recycling for ATP fluorescence detection in vitro and imaging in living cells

Gao

Yao

et al. 2018

RSC Adv.

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We developed a novel strategy for ATP detection in vitro and imaging in living cells based on integrating proximity hybridization-induced strand displacement and metal ion-dependent DNAzyme recycling amplification. Four DNA oligonucleotides were used in the sensing system including two aptamer probes, enzymatic sequences and FAM-linked substrate strands. Upon the addition of ATP, the proximity binding of two aptamers to ATP led to the release of the enzymatic sequences, which hybridized with the FAM-linked substrate strand on the graphene oxide (GO) surface to form the ion-dependent DNAzyme. Subsequent catalytic cleavage of the DNAzyme by the corresponding metal ions results in recycling of the enzymatic sequences and cyclic cleavage of the substrate strand, liberating many short FAM-linked oligonuleotide fragments separated from the GO surface, which results in fluorescence enhancement due to the weak affinity of the short FAM-linked oligonuleotide fragment to GO. The amount of produced short FAM-linked oligonuleotide fragments is positively related to the concentration of ATP. This means that one target binding could result in cleaving multiplex fluorophore labelled substrate strands, which provided effective signal amplification. The vivo studies suggested that the nanoprobe was efficiently delivered into living cells and worked for specific, high-contrast imaging of target ATP. More importantly, this target-responsive nanoscissor model is an important approach for intracellular amplified detection and imaging of various analytes by selecting appropriate affinity ligands.

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Multiple turnovers of DNAzyme for amplified detection of ATP and reduced thiol in cell homogenates

Cited by 21 publications

References 17 publications

Recent advances in cytokine detection by immunosensing

Recent advances in cytokine detection by immunosensing

Optical Aptasensors for Adenosine Triphosphate

Proximity hybridization triggered strand displacement and DNAzyme assisted strand recycling for ATP fluorescence detection in vitro and imaging in living cells

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